• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

SIGMA S Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS680013

MAb to sigma S subunit of E.coli RNA polymerase. Sigma S is a 38 kDA subunit of the E.coli RNA polymerase.Sigma S is a 38kDa transcription factor of E. coli RNA PolymerasesigmaS (sigma S) is a 38kDa subunit of the E.coli RNA polymeraseSigma transcription… More details

$590 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Sigma S, Monoclonal Antibody
Full Product Name
mAb to Sigma S
Product Gene Name
anti-Sigma S antibody
Product Synonym Names
MAb to sigmaS (sigma S) subunit of E.coli RNA polymerase; E. coli RNA Sigma S Monoclonal Antibody; Sigma 38; rpoS; appR; katF; nur; otsX; sigS
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1RS1
Host
Mouse
Species Reactivity
Most enterics
Form/Format
Ascites
Specificity
Sigma S (E. coli Sigma S (Strain MG1655))
Preparation and Storage
Store at -20 degree C
Molecular Weight
37,972 Da
Immunogen Sequence Positions
33-256
Chromosome Location
NC_000913.2 (2864581..2865573, complement)
3D Structure
ModBase 3D Structure for P13445
Immunogen
Recombinant Protein
Reported Reactivity
E Coli, Klebsiella, Salmonella, Serratia, Bordetella and an unidentified protein at 60 kDA
Cross Reactivity Note
E Coli, Klebsiella, Salmonella, Serratia, Bordetella and an unidentified protein at 60 kDA
Polyol-responsive
No
Reconstitution
Add 100ul of ddH2O to the tube containing antibody and mix to dissolve
Product Note
Does not react with Shigella, Vibrio, Pseudomonas, Rhodobacter, Agrobacterium, Streptococcus, Arthrobacter, or Borrelia *Cross reactivity and Applications data is per published literature and/or unpublished data.
Applications Tested/Suitable for anti-Sigma S antibody
Western Blot (WB), ELISA (EIA), Transcription Inhibition
Application Notes for anti-Sigma S antibody
Transcription Inhibition - Initiation: Yes
Western Blot: Yes - dilute at 1:1000

Other Notes

Small volumes of anti-Sigma S antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Sigma S purchase

MBS680013 is an antibody targeting Sigma S. Also known as mAb to Sigma S. Reactive in Most enterics. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Sigma S. It may not necessarily be applicable to this product.

NCBI GI #
16130648
NCBI GeneID
947210
NCBI Accession #
NP_417221
UniProt Primary Accession #
P13445
UniProt Secondary Accession #
Q2MA88; Q79EB7
GenBank Nucleotide #
NC_000913.2
NCBI Official Full Name
RNA polymerase, sigma S (sigma 38) factor
NCBI Official Symbol
rpoS
NCBI Official Synonym Symbols
abrD; appR; csi2; dpeB; ECK2736; JW5437; katF; nur; otsX; sigS
UniProt Protein Name
RNA polymerase sigma factor RpoS
UniProt Synonym Protein Names
Sigma S; Sigma-38
Protein Family
RNA polymerase sigma factor
UniProt Gene Name
rpoS
UniProt Synonym Gene Names
appR; katF; nur; otsX; sigS
UniProt Entry Name
RPOS_ECOLI
NCBI Protein Information
RNA polymerase, sigma S (sigma 38) factor

NCBI Summary for Sigma S

Polyamine modulon. [More information is available at EcoGene: EG10510]. rpoS encodes the alternative sigma factor S, a subunit of RNA polymerase that acts as the master regulator of the general stress response in Escherichia coli. [More information is available at EcoCyc: EG10510].

UniProt Comments for Sigma S

Function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the master transcriptional regulator of the stationary phase and the general stress response. Controls, positively or negatively, the expression of several hundred genes, which are mainly involved in metabolism, transport, regulation and stress management.

Subunit structure: Interacts with the RNA polymerase core enzyme and RssB.

Subcellular location: Cytoplasm

Potential.

Induction: Subject to complex regulation at multiple levels (transcription, translation, regulation of activity and degradation). Transcription is induced during entry into stationary phase and in response to different stresses. mRNA stability is regulated by small RNA regulators and various proteins such as Hfq, CsdA, CspC and CspE. Finally, the cellular level of RpoS is regulated by proteolysis via RssB and the ClpXP protease.

Sequence similarities: Belongs to the sigma-70 factor family. RpoS subfamily.

Sequence caution: The sequence BAE76818.1 differs from that shown. Reason: Erroneous termination at position 33. Translated as Gln.The sequence CAA34435.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence CAA78692.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
Show more

Research Articles on Sigma S

1. These results suggest that rpoS does have an influence on both biofilm formation and survival of Escherichia coli O157:H7 and that the advantage conferred by rpoS is contingent on the environmental conditions.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000