Rabbit Anti-KRT20 Monoclonal Antibody – Cat# MBS684193

Colorectal adenocarcinoma with diffuse strong expression of CK20 (A and B) and colorectal adenocarcinoma and normal epithelium of the large intestine mucosa with expression of CK20 (C). Adenoma of the large intestine with expression of CK20 (D). All, formalin fixed, paraffin embedded human tissues (4 um sections) stained with anti - Cytokeratin 20 (MBS684193) monospecific clonal antibody according to related datasheet.

Cytokeratin 20 (KRT20) Antibody – Rabbit Monoclonal

Name: KRT20 Antibody; Monoclonal; Rabbit; MyBioSource
Brand: MyBioSource
SKU: MBS684193
Price: 265 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS684193

MBS684193 is an antibody targeting KRT20. Also known as Anti - Cytokeratin 20. Host: Rabbit. More details

$265 / 1 mL (RTU) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Cytokeratin 20 (KRT20), Monoclonal Antibody

Full Product Name

Anti - Cytokeratin 20

Product Gene Name

anti-KRT20 antibody

Clonality

Monoclonal

Clone Number

E19-l

Host

Rabbit

Form/Format

20 mM Tris-HCl, pH 8.0

Specificity

Human

Preparation and Storage

Store at +4°C. Do not freeze!

Chromosome Location

Chromosome: 17; NC_000017.11 (40875889..40885243, complement). Location: 17q21.2

OMIM

608218

3D Structure

ModBase 3D Structure for P35900

Immunogen

Peptide derived from C-terminal region of human cytokeratin 20. Antibody recognizes the epitope between Val408 - Ile424.

Stabilizer

20 mg/ml BSA

Preservative

0.05% NaN₃

Cellular localization

cytoplasm

Positive control

colon adenocarcinoma tissue, Merkel cell carcinoma

PRECAUTIONS

1. We strongly recommend to use Primary Antibody Diluent (Please Inquire), eventually alternative diluent (containing protease free BSA at the concentrations > 1mg/ml) for dilution of concentrated antibodies, otherwise the warranty might be voided.
2. Centrifuge the vial before use.
3. Intended for use in laboratories.
4. Do not use after expiration date stamped on vial label.
5. Avoid contamination of the reagent.
6. Any discrepancies in the recommended procedures stated in the working protocol may affect the final results.
7. The reagent contains sodium azide (NaN3) which is highly toxic in higher concentrations. The concentration in the reagent (0.05%) is not considered as hazardous.
8. Disposal of waste material must be conducted in accordance with local regulations.
9. Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin.

ISO Certification

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Applications Tested/Suitable for anti-KRT20 antibody

Immunohistochemistry (IHC) - Paraffin

Application Notes for anti-KRT20 antibody

IHC-P READY-TO-USE

IHC-P PROTOCOL – INSTRUCTION MANUAL
1. Deparaffinize the section in 3 changes of xylene, 5 minutes each.
2. Wash the section in 96%, 80% and 70% ethyl alcohol for 10 minutes each.
3. Rinse in distilled water.
4. Block the endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes.
5. Wash in distilled water.
6. For antigen retrieval: immerse the slide in Tris-EDTA buffer, pH 9.0, 0.05% Tween-20* and incubate at 95ºC in water bath for 60 minutes.
7. Remove the staining to room temperature and let the slide to cool (in Tris-EDTA buffer, pH 9.0) for 15 minutes.
8. Rinse in distilled water.
9. Wash in 0.05 M Tris-HCl, pH 7.6 buffer supplemented with 0.2% of Tween-20 (buffer A) for 5 minutes.
10. CONCENTRATED:
Incubate the section with primary antibody at the dilution 1:100 - 1:200 for 1 hour in the closed wet chamber.
READY TO USE (RTU):
Incubate the section with primary antibody (ready to use) for 1 hour in a closed wet chamber.
11. Wash twice 5 minutes with buffer A.
12. Apply the secondary antibody (the protocol depends on the supplier), and proceed to standard immunohistochemistry protocol (HRP - Peroxide - DAB). Micropolymer-HRP detection kit rabbit/mouse dual is suggested (Please Inquire).
13. Wash twice 5 minutes with buffer A.
14. Apply the chromogen (DAB), 1 - 3 minutes.
15. Wash in water – 10 minutes.
16. Stain in hematoxylin for 5 minutes.
17. Wash in water – 10 minutes.
18. Dehydrate the section in 2 changes of 96% ethyl alcohol for 5 minutes each.
19. Wash the section in 2 changes of xylene for 2 minutes each.
20. Mount the slide for observation.

* Tris-EDTA Buffer (10mM Tris Base, 1mM EDTA solution, 0.05% Tween-20, pH 9.0):
Tris ------------- 1.21 g; EDTA ------------ 0.37 g; Distilled water -------------------------- 1000 ml
Mix to dissolve in 700 ml of distilled water. Adjust pH to 9.0 with 1M HCl and then add 0.5 ml of Tween-20 and mix well. Adjust the final volume to 1 liter with distilled water. Store this solution at room temperature for 3 months or at +4ºC for longer storage.

Other Notes

Small volumes of anti-KRT20 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for KRT20. It may not necessarily be applicable to this product.

NCBI GI #

547750

NCBI GeneID

54474

NCBI Accession #

P35900.1

UniProt Primary Accession #

P35900

UniProt Secondary Accession #

B2R6W7

UniProt Related Accession #

P35900

NCBI Official Full Name

Keratin, type I cytoskeletal 20

NCBI Official Synonym Full Names

keratin 20

NCBI Official Symbol

KRT20

NCBI Official Synonym Symbols

K20; CD20; CK20; CK-20; KRT21

UniProt Protein Name

Keratin, type I cytoskeletal 20

UniProt Synonym Protein Names

Cytokeratin-20; CK-20; Keratin-20; K20; Protein IT

Protein Family

Keratin

UniProt Gene Name

KRT20

UniProt Synonym Gene Names

CK-20; K20

UniProt Entry Name

K1C20_HUMAN

NCBI Protein Information

keratin, type I cytoskeletal 20; keratin-20; protein IT; cytokeratin 20; cytokeratin-20

NCBI Summary for KRT20

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]

UniProt Comments for KRT20

Function: Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine

By similarity. Ref.4 Ref.8

Subunit structure: Heterotetramer of two type I and two type II keratins. Associates with KRT8.

Subcellular location: Cytoplasm Ref.6 Ref.7.

Tissue specificity: Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa. Ref.6 Ref.7

Developmental stage: First detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low-differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative. Ref.1

Post-translational modification: Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury

By similarity.Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228. Ref.4

Miscellaneous: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).

Sequence similarities: Belongs to the intermediate filament family.

Research Articles on KRT20

1. Its association with clinical stage indicates that CK20 might have prognostic value as a marker for detection of circulating CRC cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-KRT20 antibody

Reference Product	PubMed Publications
MUC2 antibody	>19 publications with KRT20 and MUC2
CDX2 antibody	>18 publications with KRT20 and CDX2
MUC1 antibody	>11 publications with KRT20 and MUC1
PIP antibody	>4 publications with KRT20 and PIP
UPK3A antibody	>2 publications with KRT20 and UPK3A
MUC5B antibody	>1 publications with KRT20 and MUC5B