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Estrogen Receptor Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS370412

Summary:Estrogen receptor alpha (ER) is a nuclear receptor for estrogens such as estradiol (the main endogenous human estrogen). The two different estrogen receptor proteins produced from the ESR1 and ESR2 genes are usually called the alpha and beta… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Estrogen Receptor, Monoclonal Antibody
Full Product Name
Estrogen Receptor (EP1)
Product Synonym Names
ER alpha; ESR1; sc8002; ERalpha; ERalpha; ER a; anti-Estrogen Receptor alpha; anti Estrogen Receptor alpha; anti-er; anti er; anti-ESR1; anti ESR1; anti-ERalpha; anti ERalpha; anti ER alpha; anti-ER alpha; DKFZp686N23123; ER; ERa; ESR; ESRA; Estradiol receptor; Estrogen receptor; estrogen receptor 1; NR3A1; Nuclear receptor subfamily 3 group A member 1; estrogen receptor alpha delta 3 4 5 6 7 819 2 isoform; estrogen receptor alpha delta 4 49 isoform; estrogen receptor alpha delta 4 5 6 7 654 isoform; esrogen receptor; estrogen nuclear receptor alpha; estrogen receptor alpha 3 4 5 6 7 822 isoform; estrogen receptor alpha delta 3 4 5 6 7 8 941 isoform; estrogen receptor alpha delta 4 5 6 7 8 901 isoform
Clonality
Monoclonal
Isotype
IgG
Clone Number
EP1
Host
Rabbit
Species Reactivity
Paraffin, Frozen
Form/Format
Anti-Estrogen Receptor is a Rabbit Monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Preparation and Storage
Store at 2-8 degree C
Immunogen
Recombinant protein of human estrogen receptor alpha corresponding to amino acids 1-300.
Control
Breast, Myometrium, Cervix, Fallopian Tube, Breast Carcinoma
Localization
Nuclear
Precautions
1. For professional users only. Results should be interpreted by aqualified medical professional.
2. This product contains <0.1% sodium azide (NaN3) as a preservative. Ensure proper handling procedures are used with this reagent.
3. Always wear personal protective equipment such as a laboratory coat,goggles, and gloves when handling reagents.
4. Dispose of unused solution with copious amounts of water.
5. Do not ingest reagent. If reagent is ingested, seek medical advice immediately.
6. Avoid contact with eyes. If contact occurs, flush with large quantities of water.
7. Follow safety precautions of the heating device used for epitope retrieval (TintoRetriever Pressure Cooker or similar).
8. For additional safety information refer to the Safety Data Sheet for this product.
9. For complete recommendations for handling biological specimens, please refer to the CDC document, "Guidelines for Safe Work Practices in Human and Animal Medical Diagnostic Laboratories" (see References in this document).
Stability
This product is stable up to the expiration date on the product label. Do not use after expiration date listed on package label. Temperature fluctuations should be avoided. Store appropriately when not in use and avoid prolonged exposure to room temperature conditions
Specimen Preparation
Paraffin sections: The antibody can be used on formalin-fixed paraffin-embedded (FFPE) tissue sections. Ensure tissue undergoes appropriate fixation for best results. Pre-treatment of tissues with heat-induced epitope retrieval (HIER) is recommended using ImmunoDNA Retriever with Citrate, ImmunoDNA Retriever with EDTA, or ImmunoDNA Digestor. See reverse side for complete protocol. Tissue should remain hydrated via use of Immuno/DNA Washer solutions
Frozen sections and cell preparations
The antibody can be used on acetone-fixed frozen sections and acetone-fixed cell preparations
ISO Certification
Manufactured in an ISO 13485:2016 Certified Laboratory.
Application Notes for anti-Estrogen Receptor antibody
Breast Cancer, Carcinomas of Unknown Primary Site, Endometrial Genital Cancer

This antibody is intended for use in Immunohistochemical applications on formalin-fixed paraffin-embedded tissues (FFPE), frozen tissue sections and cell preparations. Interpretation of results should be performed by a qualified medical professional.

IHC Protocol:
1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positively charged slides such as Bio SB Hydrophilic Plus Slides.
2. Air dry for 2 hours at 58°C.
3. Deparaffinize, dehydrate, and rehydrate tissues.
4. Subject tissues to heat induced epitope retrieval (HIER) using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate
5. Any of three heating methods may be used:

a. TintoRetriever Pressure Cooker or Equivalent:
Place tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA and place on trivet in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature.

b. TintoRetriever PT Module or Water Bath Method
Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA at 95°-99°C. Incubate for 30-60 minutes.

c. Conventional Steamer Method:
Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a steamer, cover and steam for 30-60 minutes.
6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.
7. For manual IHC, perform antibody incubation at ambient temperature. For automated IHC methods, perform antibody incubation according to instrument manufacturer’s instructions.
8. Wash slides with ImmunoDNA washer or DI water.
9. Continue IHC protocol. Wash slides between each step with ImmunoDNA washer solution.

Dilution Information of anti-Estrogen Receptor antibody

Rabbit Anti-Estrogen Receptor Monoclonal Antibody – Cat# MBS370412

Immunohistochemistry (IHC) of anti-Estrogen Receptor antibody

Estrogen Receptor Alpha Antibody Immunohistochemistry of on an FFPE Breast Carcinoma Tissue
Rabbit Anti-Estrogen Receptor Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS370412

Other Notes

Small volumes of anti-Estrogen Receptor antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Estrogen Receptor purchase

MBS370412 is an antibody targeting Estrogen Receptor. Also known as Estrogen Receptor (EP1). Reactive in Paraffin, Frozen. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Estrogen Receptor. It may not necessarily be applicable to this product.

Protein Family
Estrogen receptor

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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