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PSA Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS684304

MBS684304 is an antibody targeting PSA. Also known as Anti-PSA (Prostate-specific antigen). Host: Rabbit. More details

$265 / 1 mL (RTU) +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PSA (Prostate-specific antigen), Monoclonal Antibody
Full Product Name
Anti-PSA (Prostate-specific antigen)
Product Gene Name
Clonality
Monoclonal
Clone Number
L15-V
Host
Rabbit
Form/Format
20 mM Tris-HCl, pH 8.0; 20 mg/ml BSA; 0.05% NaN3
Specificity
Human, mouse, rat
Preparation and Storage
Store at 4°C. Do not freeze.
3D Structure
ModBase 3D Structure for P07288
Immunogen
Peptide derived from N-terminal sequence of human PSA. Antibody recognizes the epitope between Arg77-Gln88
Cellular Localization
Cytoplasm
Positive Control
Prostate hyperplasia
Protein Accession Number
P07288
Ventana Protocol
Procedure: U ultraView DAB
1. Deparafinization
2. Heating (72°C) at the medium temperatures. Deparafinization
3. Cell conditioning
4. ULTRA conditioner #1
5. Heating glass (95°C), incubation 8 min. (Cell conditioner #1; buffer CC1)
6. ULTRA CC1 solution application - 36 min.
7. Antibody incubation temperature
8. Heating glass (36°C), incubation 4 min.
9. Titration
10. Hand apply - primary antibody 100 ul. Incubation 36 min.
11. ultraWash
12. Nuclear stain
13. Hematoxylin II application - one drop (nuclear stain). Cover and incubate 12 min.
14. After nuclear stain
15. Bluing reagent application, one drop. After nuclear stain, cover and incubate 4 min.
Leica Bond Max Protocol
Protocol F
Visualization system: BOND Refine DS9800
Epitope retrieval/heating time/temperature: ER2/30 min./100°C
Incubation of primary antibody/temperature: 30 min./20°C
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Applications Tested/Suitable for anti-PSA antibody
Immunohistochemistry (IHC)
Application Notes for anti-PSA antibody
IHC-P, dilution 1:100

Immunohistochemistry (IHC) of anti-PSA antibody

Ventana BenchMark; Immunohistochemical staining patterns of formalin fixed and paraffin embedded human prostate tissue (4 um sections) with Anti - PSA monospecific antibody, according to datasheet, using Ventana BenchMark. The prostate gland tissues show a strong distinct cytoplasmic PSA expression.
Rabbit Anti-PSA Monoclonal Antibody – Cat# MBS684304

Immunohistochemistry (IHC) of anti-PSA antibody

Leica Bond-Max; Immunohistochemical staining patterns of formalin fixed and paraffin embedded human prostate tissue (4 um sections) with Anti - PSA monospecific antibody, according to datasheet, using Leica Bond-Max. The prostate gland tissues show a strong distinct cytoplasmic PSA expression.
Rabbit Anti-PSA Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS684304

Other Notes

Small volumes of anti-PSA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PSA. It may not necessarily be applicable to this product.

UniProt Primary Accession #

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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