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CXCR2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS245237

The IL8RB is a Chemokine Receptor also known as type 1 interleukin-8 receptor. There are three interleukin-8 receptors on chromosome 2: IL8RA (type 2), IL8RB (type 1), and a pseudogene. Results from mouse knock-out studies suggest that IL8RB may inhibit the… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CXCR2, Monoclonal Antibody
Full Product Name
Mouse Monoclonal (IgG1) to Human CXCR2
Product Gene Name
anti-CXCR2 antibody
Product Synonym Names
CXCR2 | CDw128b | CD182 antigen | CMKAR2 | CXCR-2 | CD182 | CXC-R2 | IL-8R B | Il-8 receptor b | IL-8 receptor type 2 | Il-8rb | IL8R2 | IL8RB | Interleukin-8 receptor type B | GRO/MGSA receptor | Interleukin 8 receptor beta | Interleukin 8 receptor, beta | Chemokine (CXC) receptor 2 | IL8RA | Interleukin 8 receptor B | Interleukin 8 receptor type 2
Clonality
Monoclonal
Isotype
IgG1
Host
Mouse
Species Reactivity
Human
Form/Format
PBS.
Purity/Purification
Protein G Purified
Concentration
1 mg/ml (lot specific)
Specificity
Recognizes human IL-8 receptor, type B.
Preparation and Storage
Short term: store at 4°C. Long term: aliquot and store -20°C for up to 12 months. Avoid freeze-thaw cycles.
OMIM
146928
3D Structure
ModBase 3D Structure for P25025
Target Species
Human
Immunogen Description
L1.2 cells expressing human IL-8 (interleukin-8) receptor, type B.
Immunogen Type
Cells
Immunogen
CXCR2 antibody was raised against l1.2 cells expressing human IL-8 (interleukin-8) receptor, type B.
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip

Product Categories/Family for anti-CXCR2 antibody

Family: GPCR; Subfamily: Chemokine
Applications Tested/Suitable for anti-CXCR2 antibody
Immunohistochemistry (IHC), Immunohistochemistry (IHC - Paraffin), Flow Cytometry (FC/FACS)
Application Notes for anti-CXCR2 antibody
IHC-P: (2.5-ug/ml)
Immunohistochemistry: MBS245237 was validated for use in Immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS245237 was determined to be 2.5-5 ug/ml.

(Optimal dilution to be determined by the researcher)

Immunohistochemistry (IHC) of anti-CXCR2 antibody

Anti-CXCR2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-CXCR2 Monoclonal Antibody – Cat# MBS245237

Other Notes

Small volumes of anti-CXCR2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CXCR2 purchase

MBS245237 is an antibody targeting CXCR2. Also known as Mouse Monoclonal (IgG1) to Human CXCR2. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CXCR2. It may not necessarily be applicable to this product.

NCBI GI #
4504683
NCBI GeneID
3579
NCBI Accession #
NP_001548.1
UniProt Primary Accession #
P25025
UniProt Secondary Accession #
Q8IUZ1; Q9P2T6; Q9P2T7
UniProt Related Accession #
P25025; AAA15417; AAB24742
GenBank Nucleotide #
NM_001557.3
NCBI Official Full Name
C-X-C chemokine receptor type 2
NCBI Official Synonym Full Names
chemokine (C-X-C motif) receptor 2
NCBI Official Symbol
CXCR2
NCBI Official Synonym Symbols
CD182; IL8R2; IL8RA; IL8RB; CMKAR2; CDw128b
UniProt Protein Name
C-X-C chemokine receptor type 2
UniProt Synonym Protein Names
CDw128b; GRO/MGSA receptor; High affinity interleukin-8 receptor B; IL-8R B; IL-8 receptor type 2; CD_antigen: CD182
Protein Family
C-X-C chemokine receptor
UniProt Gene Name
CXCR2
UniProt Synonym Gene Names
IL8RB; CXC-R2; CXCR-2; IL-8R B
UniProt Entry Name
CXCR2_HUMAN
NCBI Protein Information
C-X-C chemokine receptor type 2; CXC-R2; CXCR-2; IL-8R B; GRO/MGSA receptor; IL-8 receptor type 2; interleukin 8 receptor B; chemokine (CXC) receptor 2; interleukin 8 receptor, beta; interleukin 8 receptor type 2; interleukin-8 receptor type B; CXCR2 gene for IL8 receptor type B; high affinity interleukin-8 receptor B

NCBI Summary for CXCR2

The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. This receptor also binds to chemokine (C-X-C motif) ligand 1 (CXCL1/MGSA), a protein with melanoma growth stimulating activity, and has been shown to be a major component required for serum-dependent melanoma cell growth. This receptor mediates neutrophil migration to sites of inflammation. The angiogenic effects of IL8 in intestinal microvascular endothelial cells are found to be mediated by this receptor. Knockout studies in mice suggested that this receptor controls the positioning of oligodendrocyte precursors in developing spinal cord by arresting their migration. This gene, IL8RA, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. Alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2009]
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UniProt Comments for CXCR2

IL-8R B: Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2. Belongs to the G-protein coupled receptor 1 family.

Protein type: Receptor, cytokine; GPCR, family 1; Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: cell surface; mast cell granule; membrane; integral to plasma membrane; plasma membrane; intracellular

Molecular Function: signal transducer activity; protein binding; interleukin-8 receptor activity; interleukin-8 binding; C-X-C chemokine receptor activity

Biological Process: neutrophil chemotaxis; neutrophil activation; signal transduction; chemotaxis; positive regulation of vascular permeability; negative regulation of neutrophil apoptosis; elevation of cytosolic calcium ion concentration; positive regulation of angiogenesis; cell surface receptor linked signal transduction; receptor internalization; midbrain development; positive regulation of cell proliferation; dendritic cell chemotaxis; cellular defense response; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); inflammatory response; acute inflammatory response to antigenic stimulus
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Research Articles on CXCR2

1. CXCR1 and CXCR2 (rs4674258C/T, rs1008563C/T, rs6723449T/C) were analyzed for association with IL8 levels and with myocardial infarct risk.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CXCR2 antibody

 Reference Product  PubMed Publications
 CXCR1 antibody  >505 publications with CXCR2 and CXCR1
 CXCL1 antibody  >325 publications with CXCR2 and CXCL1
 CXCL2 antibody  >151 publications with CXCR2 and CXCL2
 CXCL5 antibody  >87 publications with CXCR2 and CXCL5
 IL8 antibody  >73 publications with CXCR2 and IL8
 CXCL6 antibody  >24 publications with CXCR2 and CXCL6
 CXCL3 antibody  >12 publications with CXCR2 and CXCL3
 PPBP antibody  >11 publications with CXCR2 and PPBP
 VASP antibody  >1 publications with CXCR2 and VASP

Pathways associated with anti-CXCR2 antibody

 Products by Pathway  Pathway Diagram
 Chemokine Receptors Bind Chemokines Pathway antibodies  Chemokine Receptors Bind Chemokines Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies  Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram

Diseases associated with anti-CXCR2 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >501 publications with CXCR2 and Inflammation
 Lung Diseases Antibodies  >186 publications with CXCR2 and Lung Diseases
 Disease Models, Animal Antibodies  >160 publications with CXCR2 and Disease Models, Animal
 Necrosis Antibodies  >127 publications with CXCR2 and Necrosis
 Sepsis Antibodies  >60 publications with CXCR2 and Sepsis
 Asthma Antibodies  >48 publications with CXCR2 and Asthma
 Liver Diseases Antibodies  >39 publications with CXCR2 and Liver Diseases
 Adenocarcinoma Antibodies  >38 publications with CXCR2 and Adenocarcinoma
 Fibrosis Antibodies  >37 publications with CXCR2 and Fibrosis
 Neoplasms, Experimental Antibodies  >35 publications with CXCR2 and Neoplasms, Experimental

Organs/Tissues associated with anti-CXCR2 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >763 publications with CXCR2 and Blood
 Lung Antibodies  >238 publications with CXCR2 and Lung
 Bone Antibodies  >128 publications with CXCR2 and Bone
 Brain Antibodies  >94 publications with CXCR2 and Brain
 Intestine Antibodies  >42 publications with CXCR2 and Intestine
 Heart Antibodies  >38 publications with CXCR2 and Heart
 Spleen Antibodies  >31 publications with CXCR2 and Spleen
 Prostate Antibodies  >26 publications with CXCR2 and Prostate
 Connective Tissue Antibodies  >24 publications with CXCR2 and Connective Tissue
 Trachea Antibodies  >6 publications with CXCR2 and Trachea
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