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IL-6R Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS488649

Recombinant monoclonal antibody to IL-6R. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma D7715A7.This antibody was raised by immunising Lewis rats with OKT4 cells. More details

$635 / 0.2 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
IL-6R, Monoclonal Antibody
Full Product Name
Anti-IL-6R [D7715A7]
Product Gene Name
anti-IL-6R antibody
Product Synonym Names
CD126; 15A7; IL-6Ra; IL6R; Interleukin-6 receptor subunit alpha; Il6ra; IL-6 receptor subunit alpha; IL-6R subunit alpha; IL-6R-alpha; IL-6RA; IL-6R 1
Clonality
Monoclonal
Isotype
Rat IgG2b, kappa
Clone Number
D7715A7
Host
Rat
Species Reactivity
Mouse
Form/Format
PBS with 0.02% Proclin 300.
Purity/Purification
Purified antibody. Protein A affinity purified
Concentration
1mg/ml (lot specific)
Specificity
This antibody is specific for murine IL-6R.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Molecular Weight
50,455 Da
3D Structure
ModBase 3D Structure for P22272
Animal-free Manufacturing Platform
The recombinant antibody is manufactured using a recombinant, mammalian HEK293-based expression platform. The manufacturing platform is animal-free and animal component-free.
PMID
2073815

Product Categories/Family for anti-IL-6R antibody

oncology; cytokine signalling
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-IL-6R antibody
Blocking (BL), Flow Cytometry (FC/FACS)
Application Notes for anti-IL-6R antibody
This antibody binds to IL-6R-positive cells, and blocks IL-6 binding (Vink et al, 1990; Coulie et al, 1990). This antibody is able to inhibit the growth of IL6-dependent plasmacytoma T1033C2 in vitro (Coulie et al, 1990). Administration of this antibody to mice prior to challenge with T1033C2 resulted in a protection rate of 60% and prolonged survival in tumour-bearing mice (Vink et al, 1990). This antibody has been used in flow cytometry to assess IL-6Ra expression (Hoge et al, 2013). When added to dendritic cells subject to CD40 activation in vitro, this antibody neutralises IL-6 and ablates the effect of CD40 activation (Grohmann et al, 2001).

Immunofluorescence (IF) of anti-IL-6R antibody

Immunofluorescence staining of mouse splenocytes using anti-IL-6R antibody (MBS488648) D7715A7. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix cover-slips and stained with the chimeric rabbit IgG version of D7715A7 (MBS488648) at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488648, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (MBS488041) followed by Alexa Fluor 488 secondary antibody.
Rat Anti-IL-6R Monoclonal Antibody – Cat# MBS488649

Flow Cytometry (FC/FACS) of anti-IL-6R antibody

Flow-cytometry using the Anti-IL-6R antibody D7715A7 (MBS488648). Mouse splenocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, shaded line) or the rabbit IgG version of D7715A7 (MBS488648, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Rat Anti-IL-6R Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS488649

Product References and Citations for anti-IL-6R antibody

Coulie et al. A monoclonal antibody specific for the murine IL-6-receptor inhibits the growth of a mouse plasmacytoma in vivo. Curr Top Microbiol Immunol. 1990;166:43-6.
Note on publication: Describes the original generation of this antibody, and the characterisation of its ability to block IL-6 binding.

Other Notes

Small volumes of anti-IL-6R antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL-6R purchase

MBS488649 is an antibody targeting IL-6R. Also known as Anti-IL-6R [D7715A7]. Reactive in Mouse. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for IL-6R. It may not necessarily be applicable to this product.

NCBI GI #
110431356
NCBI GeneID
16194
NCBI Accession #
NP_034689.2
UniProt Primary Accession #
P22272
UniProt Related Accession #
P22272
GenBank Nucleotide #
NM_010559.3
NCBI Official Full Name
interleukin-6 receptor subunit alpha isoform 1
NCBI Official Synonym Full Names
interleukin 6 receptor, alpha
NCBI Official Symbol
Il6ra
NCBI Official Synonym Symbols
Il6r; CD126; IL-6R; IL-6RA; IL-6R-alpha
UniProt Protein Name
Interleukin-6 receptor subunit alpha
UniProt Synonym Protein Names
IL-6R 1; CD_antigen: CD126
UniProt Gene Name
Il6ra
UniProt Synonym Gene Names
Il6r; IL-6 receptor subunit alpha; IL-6R subunit alpha; IL-6R-alpha; IL-6RA
UniProt Entry Name
IL6RA_MOUSE
NCBI Protein Information
interleukin-6 receptor subunit alpha

UniProt Comments for IL-6R

IL6R: Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis. Belongs to the type I cytokine receptor family. Type 3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, cytokine

Cellular Component: apical plasma membrane; cell surface; extracellular region; extracellular space; integral to membrane; interleukin-6 receptor complex; membrane

Molecular Function: ciliary neurotrophic factor receptor activity; enzyme binding; hematopoietin/interferon-class (D200-domain) cytokine receptor activity; interleukin-6 binding; interleukin-6 receptor activity; interleukin-6 receptor binding; protein homodimerization activity

Biological Process: cell growth; cytokine and chemokine mediated signaling pathway; developmental growth; endocrine pancreas development; negative regulation of collagen biosynthetic process; positive regulation of cell proliferation; positive regulation of chemokine production; positive regulation of interleukin-6 production; positive regulation of JAK-STAT cascade; positive regulation of MAPKKK cascade; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of smooth muscle cell proliferation; positive regulation of tyrosine phosphorylation of Stat3 protein; response to cytokine stimulus
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Research Articles on IL-6R

1. established that endogenous IL-6R of both human and murine origin is shed by ADAM17 in an induced manner, whereas constitutive release of endogenous IL-6R is largely mediated by ADAM10

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-IL-6R antibody

 Reference Product  PubMed Publications
 Jak1 antibody  >1 publications with IL-6R and Jak1
 Il6st antibody  >1 publications with IL-6R and Il6st
 Il6 antibody  >1 publications with IL-6R and Il6

Pathways associated with anti-IL-6R antibody

 Products by Pathway  Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 HIF-1 Signaling Pathway antibodies  HIF-1 Signaling Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 IL-6 Signaling Pathway antibodies  IL-6 Signaling Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Interleukin-6 Family Signaling Pathway antibodies  Interleukin-6 Family Signaling Pathway Diagram
 Interleukin-6 Signaling Pathway antibodies  Interleukin-6 Signaling Pathway Diagram

Diseases associated with anti-IL-6R antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >4 publications with IL-6R and Inflammation
 Disease Models, Animal Antibodies  >2 publications with IL-6R and Disease Models, Animal
 Brain Diseases Antibodies  >1 publications with IL-6R and Brain Diseases
 Immune System Diseases Antibodies  >1 publications with IL-6R and Immune System Diseases
 Hypertrophy Antibodies  >1 publications with IL-6R and Hypertrophy

Organs/Tissues associated with anti-IL-6R antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >3 publications with IL-6R and Brain
 Muscle Antibodies  >1 publications with IL-6R and Muscle
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