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BAFF Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS690156

BAFF, also known as Blymphocyte stimulator (BLyS), TNFand APO-Lrelated leukocyte expressed ligand 1 (TALL1), and TNF homolog that activates apoptosis, NFKB and JNK (THANK), is a TNF ligand superfamily member and has been designated TNFSF13B. More details

$605 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
BAFF, Monoclonal Antibody
Full Product Name
Anti-Human BAFF
Product Gene Name
Product Synonym Names
Monoclonal antibody anti-human BAFF
Clonality
Monoclonal
Isotype
IgG1, kappa
Host
Mouse
Species Reactivity
Human
Form/Format
Lyophilized
Purity/Purification
Protein G chromatography
Preparation and Storage
Stability: Lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least 2 weeks at 2-8 degree C. Frozen aliquots are stable for at least 6 months when stored at -20 degree C.
Molecular Weight
38,214 Da
Chromosome Location
Chromosome: 3; NC_000003.12 (98732236..98795845). Location: 3q12.1
OMIM
607156
3D Structure
ModBase 3D Structure for Q9Y274
Immunogen
highly pure (>98%) recombinant human BAFF
Antibody Generation
Monoclonal antibodies were produced in BALB/c mice using recombinant human BAFF as the immunizing antigen. This IgG1/K antibody was purified from ascites fluid on a protein A chromatography column.
Protein RefSeq
NP_006564.1
Uniprot ID
Q9Y274
mRNA RefSeq
NM_006573.4
Buffer
PBS
Reconstitution buffer
water
Reconstitution
Centrifuge vial prior to opening. Reconstitute the antibody in sterile water to a concentration of 0.1 - 1.0 mg/ml.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-BAFF antibody
ELISA; Western Blot (WB)
Application Notes for anti-BAFF antibody
ELISA:
In a Sandwich ELISA (assuming 100 ?l/well), a concentration of 5-6 ug/ml of this antibody will detect at least 1000 pg/ml of recombinant Human BAFF when used in conjunction with an appropriate conjugated secondary antibody as detection system.

Western Blot:
To detect hBAFF by Western Blot analysis this antibody can be used at a concentration of 0.25 - 0.50 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant hBAFF is 1 - 2 ng/lane, under reducing and non-reducing conditions.

Other Notes

Small volumes of anti-BAFF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BAFF purchase

MBS690156 is an antibody targeting BAFF. Also known as Anti-Human BAFF. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for BAFF. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
type 2 lactosamine alpha-2,3-sialyltransferase isoform 3
NCBI Official Synonym Full Names
ST3 beta-galactoside alpha-2,3-sialyltransferase 6
NCBI Official Symbol
ST3GAL6
NCBI Official Synonym Symbols
SIAT10; ST3GALVI
UniProt Protein Name
Type 2 lactosamine alpha-2,3-sialyltransferase
UniProt Synonym Protein Names
CMP-NeuAc:beta-galactoside alpha-2,3-sialyltransferase VI; ST3Gal VI; ST3GalVI; Sialyltransferase 10
UniProt Gene Name
ST3GAL6
UniProt Synonym Gene Names
SIAT10; ST3GalVI
UniProt Entry Name
SIA10_HUMAN
NCBI Protein Information
type 2 lactosamine alpha-2,3-sialyltransferase; alpha2,3-sialyltransferase ST3Gal VI; sialyltransferase 10 (alpha-2,3-sialyltransferase VI); CMP-NeuAc:beta-galactoside alpha-2,3-sialyltransferase VI

NCBI Summary for BAFF

The protein encoded by this gene is a member of the sialyltransferase family. Members of this family are enzymes that transfer sialic acid from the activated cytidine 5'-monophospho-N-acetylneuraminic acid to terminal positions on sialylated glycolipids (gangliosides) or to the N- or O-linked sugar chains of glycoproteins. This protein has high specificity for neolactotetraosylceramide and neolactohexaosylceramide as glycolipid substrates and may contribute to the formation of selectin ligands and sialyl Lewis X, a carbohydrate important for cell-to-cell recognition and a blood group antigen. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Sep 2012]
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UniProt Comments for BAFF

Function: Involved in the synthesis of sialyl-paragloboside, a precursor of sialyl-Lewis X determinant. Has a alpha-2,3-sialyltransferase activity toward Gal-beta1,4-GlcNAc structure on glycoproteins and glycolipids. Has a restricted substrate specificity, it utilizes Gal-beta1,4-GlcNAc on glycoproteins, and neolactotetraosylceramide and neolactohexaosylceramide, but not lactotetraosylceramide, lactosylceramide or asialo-GM1.

Subcellular location: Golgi apparatus membrane; Single-pass type II membrane protein

Potential.

Tissue specificity: Ubiquitous.

Sequence similarities: Belongs to the glycosyltransferase 29 family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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