Dectin-2 (CLEC4N) Antibody – Rat Monoclonal

Dectin2alpha or Dectin2, also known as CLEC6A, CLECSF10, and NKCL, belongs to the Ctype lectin family of transmembrane immune regulatory glycoproteins. Dectin 2alpha, CLEC4A/DCIR, CLEC4B/DCAR, CLEC4C/DLEC, CLEC4D/MCL, and CLEC4E/mincle constitute a subgroup of these molecules that exhibit approximately 40% amino acid (aa) sequence identity in their extracellular domains (ECD) and have a conserved cysteine spacing in their carbohydrate recognition domains (CRD). Mature mouse Dectin2alpha consists of a short cytoplasmic tail, a transmembrane segment, and an ECD with a stalk region and one CRD. Alternate splicing leads to partial deletion of the transmembrane segment and stalk (beta isoform) or a portion of the CRD (gamma isoform). The full length Dectin2alpha isoform is a 27 kDa molecule that is primarily expressed on the surface of tissue macrophages and their precursors. The CRD of Dectin2alpha contains an EPN motif which is characteristic of calciumdependent mannosebinding lectins. Dectin2alpha selectively interacts with high mannose structures in the Man9GlcNAc2 configuration. It mediates the recognition of a variety of microorganisms, particularly the filamentous forms of yeast and fungii. The short cytoplasmic tail does not contain signaling motifs but mediates association with the ITAMcontaining Fc receptor gamma subunit in macrophages. Ligation of Dectin2alpha induces tyrosine phosphorylation of the gamma subunit, activation of NFkappaB, and enhanced release of TNFalpha and IL1ra. Macrophage Dectin2alpha is upregulated in vivo by inflammatory stimuli and UVB irradiation. It mediates the breaking of UVinduced tolerance by interacting with CD4+CD25+ regulatory T cells which then induce dendritic cells to release IL4, IL10, and TGFbeta. Within the ECD, mouse Dectin2alpha shares 71% aa sequence identity with human and bovine Dectin2.

Small volumes of anti-Clec4n antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS690181 is an antibody targeting Clec4n. Also known as Rat Anti-Mouse Dectin-2. Reactive in Mouse. Host: Rat.