Rat Anti-TIM-3 Monoclonal Antibody – Cat# MBS690232

T-Cell Immunoglobulin Mucin-3 (TIM-3) Antibody – Rat Monoclonal

Name: TIM-3 Antibody; Monoclonal; Rat; Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS690232
Price: 875 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS690232

T cell immunoglobulin mucin-3 (TIM-3) is a member of the T-cell Immunoglobulin- and Mucin-domain-containing family of type I membrane glycoproteins that regulate autoimmune and allergic disease. TIM-3 is selectively expressed on Th1 cells and interacts with… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

T-cell Immunoglobulin Mucin-3 (TIM-3), Monoclonal Antibody

Full Product Name

Rat Anti-Mouse T-cell Immunoglobulin Mucin-3 (TIM-3)

Product Gene Name

anti-TIM-3 antibody

Product Synonym Names

Anti-mouse TIM-3 [T-cell Immunoglobulin Mucin-3] (#6F46)

Clonality

Monoclonal

Isotype

IgG2

Clone Number

(6F46)

Host

Rat

Species Reactivity

Mouse

Form/Format

The IgG fraction of culture supernatant was purified by Protein A/G affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS). Lyophilized samples are stable for 2 years from date of receipt when stored at

Purity/Purification

Protein A/G affinity chromatography

Specificity

This antibody detects mouse of TIM-3 in Western blotting. This antibody does not cross-react with mouse TIM-1 and TIM-2.

Preparation and Storage

Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity.

Molecular Weight

30,934 Da

Chromosome Location

Chromosome: 11; NC_000077.6 (46454452..46481255). Location: 11 B1.1; 11

3D Structure

ModBase 3D Structure for Q8VIM0

Antibody Generation

This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse recombinant TIM-3. The IgG2 fraction of the culture supernatant was purified by Protein A/G affinity chromatography.

Reconstitution

Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml.

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-TIM-3 antibody

Western Blot (WB)

Application Notes for anti-TIM-3 antibody

1. Western blotting: Yes (1:500-1000)

Other Notes

Small volumes of anti-TIM-3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TIM-3 purchase

MBS690232 is an antibody targeting TIM-3. Also known as Rat Anti-Mouse T-cell Immunoglobulin Mucin-3 (TIM-3). Reactive in Mouse. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for TIM-3. It may not necessarily be applicable to this product.

NCBI GI #

160333699

NCBI GeneID

171285

NCBI Accession #

NP_599011.2

UniProt Primary Accession #

Q8VIM0

UniProt Related Accession #

Q8VIM0

GenBank Nucleotide #

NM_134250.2

NCBI Official Full Name

hepatitis A virus cellular receptor 2 homolog

NCBI Official Synonym Full Names

hepatitis A virus cellular receptor 2

NCBI Official Symbol

Havcr2

NCBI Official Synonym Symbols

Tim3; TIM-3; Timd3

UniProt Protein Name

Hepatitis A virus cellular receptor 2 homolog

UniProt Synonym Protein Names

T-cell immunoglobulin and mucin domain-containing protein 3; TIMD-3; T-cell immunoglobulin mucin receptor 3; TIM-3; T-cell membrane protein 3

UniProt Gene Name

Havcr2

UniProt Synonym Gene Names

Tim3; Timd3; HAVcr-2; TIMD-3; TIM-3

UniProt Entry Name

HAVR2_MOUSE

NCBI Protein Information

hepatitis A virus cellular receptor 2 homolog; TIMD-3; HAVcr-2; T-cell membrane protein 3; T-cell immunoglobulin mucin receptor 3; T-cell immunoglobulin and mucin domain containing 3; T-cell immunoglobulin and mucin domain-containing protein 3

UniProt Comments for TIM-3

Function: Regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Receptor for LGALS9

By similarity. Ref.3

Subcellular location: Membrane; Single-pass type I membrane protein

Potential.

Tissue specificity: Expressed in T-helper type 1 lymphocytes. Not expressed by naive T-cells but up-regulated as they differentiate into T-helper-1 cells. Also expressed by differentiated type 1 CD8+ cytotoxic T-cells. Ref.3

Miscellaneous: Belongs to the T-cell and airway phenotype regulator (Tapr) locus, a single chromosomal region that confers reduced T-helper type 2 responsiveness and protects against airway hyperactivity (AHR), the hallmark of human asthma.In vivo administration of antibody to HAVCR2 enhances the clinical and pathological severity of experimental autoimmune encephalomyelitis (EAE), a Th1-dependent autoimmune disease and increases the number and activation level of macrophages.

Sequence similarities: Belongs to the immunoglobulin superfamily. TIM family.Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Research Articles on TIM-3

1. results indicate that the Tim-3 pathway is highly involved in the regulation of asthma; targeting Tim-3 by siRNA may hold therapeutic potential in preventing the development of allergic asthma

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-TIM-3 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>55 publications with TIM-3 and Neoplasms
Inflammation Antibodies	>41 publications with TIM-3 and Inflammation
Disease Models, Animal Antibodies	>35 publications with TIM-3 and Disease Models, Animal
Hepatitis Antibodies	>28 publications with TIM-3 and Hepatitis
Lung Diseases Antibodies	>27 publications with TIM-3 and Lung Diseases
Liver Diseases Antibodies	>25 publications with TIM-3 and Liver Diseases
Neoplasms, Experimental Antibodies	>19 publications with TIM-3 and Neoplasms, Experimental
Necrosis Antibodies	>18 publications with TIM-3 and Necrosis
Cardiovascular Diseases Antibodies	>13 publications with TIM-3 and Cardiovascular Diseases
Skin Diseases Antibodies	>12 publications with TIM-3 and Skin Diseases

Organs/Tissues associated with anti-TIM-3 antibody

Organ/Tissue Name	Pubmed Publications
Spleen Antibodies	>17 publications with TIM-3 and Spleen
Brain Antibodies	>5 publications with TIM-3 and Brain
Pancreas Antibodies	>2 publications with TIM-3 and Pancreas
Uterus Antibodies	>1 publications with TIM-3 and Uterus