Rat Anti-LGALS3 Monoclonal Antibody – Cat# MBS690242

Galectin-3 (LGALS3) Antibody – Rat Monoclonal

Name: LGALS3 Antibody; Monoclonal; Rat; Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS690242
Price: 875 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS690242

The galectins constitute a large family of carbohydrate-binding proteins that function in many systems both intracellularly and following secretion. Galectins contain either one or two carbohydrate recognition domains (CRR) which mediate recognition of… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

Galectin-3 (Lgals3), Monoclonal Antibody

Full Product Name

Rat Anti-Mouse Galectin-3

Product Gene Name

anti-Lgals3 antibody

Product Synonym Names

Anti-mouse Galectin-3 (#5L36)

Clonality

Monoclonal

Isotype

IgG2

Clone Number

(5L36)

Host

Rat

Species Reactivity

Mouse

Form/Format

The IgG fraction of culture supernatant was purified by Protein A/G affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS). Lyophilized samples are stable for 2 years from date of receipt when stored at

Purity/Purification

Protein A/G affinity chromatography

Specificity

This antibody detects mouse Galectin-3 in Western blotting.

Preparation and Storage

Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity.

Molecular Weight

27,515 Da

Chromosome Location

Chromosome: 14; NC_000080.6 (47373860..47386167). Location: 14 C1; 14

3D Structure

ModBase 3D Structure for P16110

Antibody Generation

This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse recombinant Galectin-3. The IgG2 fraction of the culture supernatant was purified by Protein A/G affinity chromatography.

Reconstitution

Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml.

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-Lgals3 antibody

Western Blot (WB)

Application Notes for anti-Lgals3 antibody

1. Western blot: Yes (1:500-1000)

Other Notes

Small volumes of anti-Lgals3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Lgals3 purchase

MBS690242 is an antibody targeting Lgals3. Also known as Rat Anti-Mouse Galectin-3. Reactive in Mouse. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for Lgals3. It may not necessarily be applicable to this product.

NCBI GI #

126679

NCBI GeneID

16854

NCBI Accession #

P16110.3

UniProt Primary Accession #

P16110

UniProt Related Accession #

P16110

NCBI Official Full Name

Galectin-3

NCBI Official Synonym Full Names

lectin, galactose binding, soluble 3

NCBI Official Symbol

Lgals3

NCBI Official Synonym Symbols

GBP; L-34; gal3; Mac-2

UniProt Protein Name

Galectin-3

UniProt Synonym Protein Names

35 kDa lectin; Carbohydrate-binding protein 35; CBP 35; Galactose-specific lectin 3; IgE-binding protein; L-34 galactoside-binding lectin; Laminin-binding protein; Lectin L-29; Mac-2 antigen

Protein Family

Galectin-3-binding protein

UniProt Gene Name

Lgals3

UniProt Synonym Gene Names

Gal-3; CBP 35

UniProt Entry Name

LEG3_MOUSE

NCBI Protein Information

galectin-3; gal-3; CBP 35; lectin L-29; 35 kDa lectin; mac-2 antigen; igE-binding protein; laminin-binding protein; galactose-specific lectin 3; L-34 galactoside-binding lectin; carbohydrate-binding protein 35

UniProt Comments for Lgals3

Function: Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells

By similarity. Ref.9

Subunit structure: Probably forms homo- or heterodimers. Interacts with DMBT1

By similarity. Forms a complex with the ITGA3, ITGB1 and CSPG4. Interacts with LGALS3BP, LYPD3, CYHR1 and UACA. Ref.7 Ref.8 Ref.9

Subcellular location: Cytoplasm. Nucleus. Secreted. Note: Secreted by a non-classical secretory pathway and associates with the cell surface. Ref.9 Ref.10

Tissue specificity: The highest levels are found in activated macrophages.

Sequence similarities: Contains 1 galectin domain.

Research Articles on Lgals3

1. Fasting glucose and altered responsiveness to a glucose load preceded development of excess adiposity and systemic inflammation, as demonstrated in 12-week-old Gal-3 KO mice.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Lgals3 antibody

Reference Product	PubMed Publications
Lgals3bp antibody	>36 publications with Lgals3 and Lgals3bp
Cd68 antibody	>24 publications with Lgals3 and Cd68
S100a6 antibody	>6 publications with Lgals3 and S100a6
Gemin4 antibody	>5 publications with Lgals3 and Gemin4
Anxa2 antibody	>3 publications with Lgals3 and Anxa2
Ddost antibody	>2 publications with Lgals3 and Ddost
Prkcsh antibody	>2 publications with Lgals3 and Prkcsh
Capg antibody	>1 publications with Lgals3 and Capg
Anxa1 antibody	>1 publications with Lgals3 and Anxa1
Sufu antibody	>1 publications with Lgals3 and Sufu

Pathways associated with anti-Lgals3 antibody

Products by Pathway	Pathway Diagram
Advanced Glycosylation Endproduct Receptor Signaling Pathway antibodies	Advanced Glycosylation Endproduct Receptor Signaling Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Innate Immune System Pathway antibodies	Innate Immune System Pathway Diagram

Organs/Tissues associated with anti-Lgals3 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>507 publications with Lgals3 and Blood
Thyroid Antibodies	>277 publications with Lgals3 and Thyroid
Brain Antibodies	>124 publications with Lgals3 and Brain
Liver Antibodies	>107 publications with Lgals3 and Liver
Bone Antibodies	>105 publications with Lgals3 and Bone
Lung Antibodies	>105 publications with Lgals3 and Lung
Kidney Antibodies	>105 publications with Lgals3 and Kidney
Muscle Antibodies	>80 publications with Lgals3 and Muscle
Skin Antibodies	>79 publications with Lgals3 and Skin
Bone Marrow Antibodies	>62 publications with Lgals3 and Bone Marrow