Rat Anti-MMP-3 Monoclonal Antibody – Cat# MBS690243

MMP-3 Antibody – Rat Monoclonal

Name: MMP-3 Antibody; Monoclonal; Rat; Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS690243
Price: 875 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS690243

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP3 (stromelysin1), can degrade a broad range of substrates including collagen alpha… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

MMP-3, Monoclonal Antibody

Full Product Name

Rat Anti-Mouse MMP-3

Product Gene Name

anti-MMP-3 antibody

Product Synonym Names

Anti-mouse MMP-3 (#6C34)

Clonality

Monoclonal

Isotype

IgG2

Clone Number

(6C34)

Host

Rat

Species Reactivity

Mouse

Form/Format

The IgG fraction of culture supernatant was purified by Protein A/G affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS). Lyophilized samples are stable for 2 years from date of receipt when stored at

Purity/Purification

Protein A/G affinity chromatography

Specificity

This antibody detects mouse MMP-3 in Western blotting.

Preparation and Storage

Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity.

Molecular Weight

53,845 Da

Chromosome Location

Chromosome: 9; NC_000075.6 (7445822..7455975). Location: 9 A1; 9 2.46 cM

3D Structure

ModBase 3D Structure for P28862

Antibody Generation

This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse recombinant protein of MMP-3. The IgG2 fraction of the culture supernatant was purified by Protein A/G affinity chromatography.

Reconstitution

Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml.

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-MMP-3 antibody

Western Blot (WB)

Application Notes for anti-MMP-3 antibody

1. Western blot: Yes (1:500-1000)

Other Notes

Small volumes of anti-MMP-3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MMP-3 purchase

MBS690243 is an antibody targeting MMP-3. Also known as Rat Anti-Mouse MMP-3. Reactive in Mouse. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for MMP-3. It may not necessarily be applicable to this product.

NCBI GI #

6754714

NCBI GeneID

17392

NCBI Accession #

NP_034939.1

UniProt Primary Accession #

P28862

UniProt Related Accession #

P28862

GenBank Nucleotide #

NM_010809.1

NCBI Official Full Name

stromelysin-1 preproprotein

NCBI Official Synonym Full Names

matrix metallopeptidase 3

NCBI Official Symbol

Mmp3

NCBI Official Synonym Symbols

SLN1; Str1; SLN-1; STR-1; Stmy1

UniProt Protein Name

Stromelysin-1

UniProt Synonym Protein Names

EMS-2; Matrix metalloproteinase-3; MMP-3; Transin-1

Protein Family

Matrix metalloproteinase

UniProt Gene Name

Mmp3

UniProt Synonym Gene Names

SL-1; MMP-3

UniProt Entry Name

MMP3_MOUSE

NCBI Protein Information

stromelysin-1; SL-1; EMS-2; MMP-3; transin-1; progelatinase; stromelysin 1; matrix metalloproteinase 3; matrix metalloproteinase-3

NCBI Summary for MMP-3

This gene encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that is involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded protein is activated by the removal of an N-temrinal activation peptide to generate a zinc-dependent endopeptidase with a broad range of substrates such as proteoglycans, laminin, fibronectin, elastin, and collagens. This gene is located in a cluster of other matrix metalloproteinase genes on chromosome 9. [provided by RefSeq, Aug 2015]

UniProt Comments for MMP-3

Function: Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.

Catalytic activity: Preferential cleavage where P1', P2' and P3' are hydrophobic residues.

Cofactor: Binds 4 calcium ions per subunit

By similarity.Binds 2 zinc ions per subunit

By similarity.

Subcellular location: Secreted › extracellular space › extracellular matrix

Probable.

Developmental stage: Present in unfertilized eggs and at the zygote and cleavage stages. Levels increase at the blastocyst stage and with endoderm differentiation. Ref.3

Domain: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Sequence similarities: Belongs to the peptidase M10A family.Contains 4 hemopexin repeats.

Sequence caution: The sequence CAA44860.1 differs from that shown. Reason: Erroneous initiation.

Research Articles on MMP-3

1. Elevated expression of MMP-3 in globoid cell leukodystrophy mediates pathophysiological processes in this disease.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-MMP-3 antibody

Products by Pathway	Pathway Diagram
Activation Of Matrix Metalloproteinases Pathway antibodies	Activation Of Matrix Metalloproteinases Pathway Diagram
Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway antibodies	Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway Diagram
Collagen Degradation Pathway antibodies	Collagen Degradation Pathway Diagram
Collagen Formation Pathway antibodies	Collagen Formation Pathway Diagram
Degradation Of The Extracellular Matrix Pathway antibodies	Degradation Of The Extracellular Matrix Pathway Diagram
EGFR Transactivation By Gastrin Pathway antibodies	EGFR Transactivation By Gastrin Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram
Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies	Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
Matrix Metalloproteinases Pathway antibodies	Matrix Metalloproteinases Pathway Diagram
Rheumatoid Arthritis Pathway antibodies	Rheumatoid Arthritis Pathway Diagram

Diseases associated with anti-MMP-3 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>138 publications with MMP-3 and Neoplasms
Inflammation Antibodies	>137 publications with MMP-3 and Inflammation
Cardiovascular Diseases Antibodies	>90 publications with MMP-3 and Cardiovascular Diseases
Necrosis Antibodies	>79 publications with MMP-3 and Necrosis
Disease Models, Animal Antibodies	>69 publications with MMP-3 and Disease Models, Animal
Nervous System Diseases Antibodies	>62 publications with MMP-3 and Nervous System Diseases
Heart Diseases Antibodies	>44 publications with MMP-3 and Heart Diseases
Atherosclerosis Antibodies	>38 publications with MMP-3 and Atherosclerosis
Neoplasm Invasiveness Antibodies	>35 publications with MMP-3 and Neoplasm Invasiveness
Lung Diseases Antibodies	>32 publications with MMP-3 and Lung Diseases

Organs/Tissues associated with anti-MMP-3 antibody

Organ/Tissue Name	Pubmed Publications
Joint Antibodies	>141 publications with MMP-3 and Joint
Bone Antibodies	>97 publications with MMP-3 and Bone
Connective Tissue Antibodies	>48 publications with MMP-3 and Connective Tissue
Lung Antibodies	>40 publications with MMP-3 and Lung
Intestine Antibodies	>10 publications with MMP-3 and Intestine
Mammary Gland Antibodies	>9 publications with MMP-3 and Mammary Gland
Pancreas Antibodies	>3 publications with MMP-3 and Pancreas
Oviduct Antibodies	>1 publications with MMP-3 and Oviduct