BEC12 Antibody – Rat Monoclonal

Monoclonal

IgG2a

IgG2a, LA5

Rat

Mouse

Lyophilized

Protein G purified

LA5 reacts with most mouse blood vessels except heart, large arteries and veins, but not with any lymphatic vessels. LA5 recognized a protein of around 12kDa. The antigen recognized by LA5 was localized on both luminal and abluminal blood endothelial cell membranes. Besides blood vessels, some lymphoid cells are also recognized.

The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8 degree C. Frozen aliquots are stable for at least 6 months when stored at -20 degree C.

With collagenase- and neuraminidase-treated mouse benign lymphangiomas

The antibody was produced by a rapid differential immunization of DA rats with collagenase- and neuraminidase-treated mouse benign lymphangiomas (licensed from Tokyo Women's Medical University, Japan). Two novel rat monoclonal antibodies (LA102 and LA5) were generated to identify mouse lymphatic vessels and blood vessels, respectively. Both antibodies were characterized as to the morphological and functional specificities of endothelial cells of both types of vessels. The antibodies were produced by a rapid differential immunization of DA rats with collagenase- and neuraminidase-treated mouse lymphangioma tissues. LA5 reacted with most mouse blood vessels with a few exceptions, but not with lymphatics. LA5 recognized a molecule of 12-13 kDa. Neither antibody recognized any currently identified lymphatic or vascular endothelial cell antigens. Immunoelectron microscopy revealed that the antigens recognized by LA5 were localized on both luminal and abluminal endothelial cell membranes of each vessel type. Besides endothelial cells, both antibodies also recognized some types of lymphoid cells.

PBS

Manufactured in an ISO 9001:2008 Certified Laboratory.