Rat Anti-TSP50 Monoclonal Antibody – Cat# MBS690313

TSP50 Antibody – Rat Monoclonal

Name: TSP50 Antibody; Monoclonal; Rat; Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS690313
Price: 875 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS690313

TSP50 is a member of the peptidase S1 family of serine proteases. It is a secreted glycoprotein that may play a role in spermatogenesis and act as an oncogene. More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

TSP50 (Testes-specific protease 50), Monoclonal Antibody

Full Product Name

Rat Anti-Mouse TSP50 (Testes-specific protease 50)

Product Gene Name

anti-TSP50 antibody

Product Synonym Names

Anti-mouse Testes-Specific Protease 50 [TSP50] (#6H39)

Clonality

Monoclonal

Isotype

IgG2

Clone Number

(6H39)

Host

Rat

Species Reactivity

Mouse

Form/Format

The IgG fraction of culture supernatant was purified by Protein A/G affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS). Lyophilized samples are stable for 2 years from date of receipt when stored at

Purity/Purification

Protein A/G affinity chromatography

Specificity

This antibody detects mouse of TSP50 in Western blotting.

Preparation and Storage

Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity.

Molecular Weight

43,088 Da

Chromosome Location

Chromosome: 3; NC_000003.12 (46712116..46717883, complement). Location: 3p21.31

OMIM

607950

3D Structure

ModBase 3D Structure for Q9UI38

Antibody Generation

This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse recombinant TSP50. The IgG2 fraction of the culture supernatant was purified by Protein A/G affinity chromatography.

Reconstitution

Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml.

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-TSP50 antibody

Western Blot (WB)

Application Notes for anti-TSP50 antibody

1. Western blotting: Yes (1:500-1000)

Other Notes

Small volumes of anti-TSP50 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TSP50 purchase

MBS690313 is an antibody targeting TSP50. Also known as Rat Anti-Mouse TSP50 (Testes-specific protease 50). Reactive in Mouse. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for TSP50. It may not necessarily be applicable to this product.

NCBI GI #

7019563

NCBI GeneID

29122

NCBI Accession #

NP_037402.1

UniProt Primary Accession #

Q9UI38

UniProt Related Accession #

Q9UI38

GenBank Nucleotide #

NM_013270.4

NCBI Official Full Name

probable threonine protease PRSS50

NCBI Official Synonym Full Names

protease, serine, 50

NCBI Official Symbol

PRSS50

NCBI Official Synonym Symbols

CT20; TSP50

UniProt Protein Name

Probable threonine protease PRSS50

UniProt Synonym Protein Names

Cancer/testis antigen 20; Serine protease 50; Testis-specific protease-like protein 50

UniProt Gene Name

PRSS50

UniProt Synonym Gene Names

CT20; TSP50

UniProt Entry Name

TSP50_HUMAN

NCBI Protein Information

probable threonine protease PRSS50; serine protease 50; cancer/testis antigen 20; testes specific protease 50; testes-specific protease 50; testis-specific protease-like protein 50

UniProt Comments for TSP50

Function: May be involved in proteolysis through its threonine endopeptidase activity. Ref.3

Subcellular location: Endoplasmic reticulum

Probable. Note: May also localize to cytoplasmic membranes. Ref.3

Tissue specificity: Testis specific. Differentially expressed in some breast cancer tissues.

Miscellaneous: DNA hypomethylation is accompanied by the expression of the gene in the testis.

Sequence similarities: Belongs to the peptidase S1 family.Contains 1 peptidase S1 domain.

Caution: Although related to peptidase S1 family, lacks the conserved active Ser residue in position 310 which is replaced by a Thr.

Research Articles on TSP50

1. the importance of threonine 310, the most critical protease catalytic site in TSP50, to TSP50-induced cell proliferation and tumor formation

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-TSP50 antibody

Organ/Tissue Name	Pubmed Publications
Testis Antibodies	>1 publications with TSP50 and Testis