Rat Anti-NUS1 Monoclonal Antibody – Cat# MBS690326

Nogo Receptor (NUS1) Antibody – Rat Monoclonal

Name: NUS1 Antibody; Monoclonal; Rat; Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS690326
Price: 875 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS690326

Nogo Receptor (Nogo R, NgR), also named reticulon 4 receptor, is a glycosylphosphatidylinositol (GPI)-anchored protein that belongs to the family of leucine-rich repeat (LRR) proteins. It is expressed predominantly in the central nervous systems on neurons… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

Nogo receptor (Nus1), Monoclonal Antibody

Full Product Name

Rat Anti-Mouse Nogo receptor

Product Gene Name

anti-Nus1 antibody

Product Synonym Names

Anti-mouse Nogo receptor (#3D66)

Clonality

Monoclonal

Isotype

IgG2

Clone Number

(3D66)

Host

Rat

Species Reactivity

Mouse

Form/Format

The IgG fraction of culture supernatant was purified by Protein A/G affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS). Lyophilized samples are stable for 2 years from date of receipt when stored at

Purity/Purification

Protein A/G affinity chromatography

Specificity

This antibody detects mouse Nogo receptor in Western blotting.

Preparation and Storage

Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity.

Molecular Weight

33,485 Da

Chromosome Location

Chromosome: 10; NC_000076.6 (52417547..52440192). Location: 10 B3; 10 26.64 cM

3D Structure

ModBase 3D Structure for Q99LJ8

Antibody Generation

This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse recombinant Nogo receptor extracellular domain. The IgG2 fraction of the culture supernatant was purified by Protein A/G affinity chromatography.

Reconstitution

Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml.

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-Nus1 antibody

Western Blot (WB)

Application Notes for anti-Nus1 antibody

1. Western blot: Yes (1:500-1000)

Other Notes

Small volumes of anti-Nus1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Nus1 purchase

MBS690326 is an antibody targeting Nus1. Also known as Rat Anti-Mouse Nogo receptor. Reactive in Mouse. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for Nus1. It may not necessarily be applicable to this product.

NCBI GI #

13384840

NCBI GeneID

52014

NCBI Accession #

NP_084526.1

UniProt Primary Accession #

Q99LJ8

UniProt Secondary Accession #

Q0P6D7; Q3TIA3; Q3TIR7; Q3U1Z4

UniProt Related Accession #

Q99LJ8

GenBank Nucleotide #

NM_030250.2

NCBI Official Full Name

nogo-B receptor

NCBI Official Synonym Full Names

nuclear undecaprenyl pyrophosphate synthase 1 homolog (S. cerevisiae)

NCBI Official Symbol

Nus1

NCBI Official Synonym Symbols

AU019165; AW538011; BC003223; D10Ertd438e; 1600027K07Rik

UniProt Protein Name

Nogo-B receptor

UniProt Synonym Protein Names

Nuclear undecaprenyl pyrophosphate synthase 1 homolog

Protein Family

Dehydrodolichyl diphosphate synthase complex

UniProt Gene Name

Nus1

UniProt Synonym Gene Names

D10Ertd438e; Ngbr; NgBR

UniProt Entry Name

NGBR_MOUSE

NCBI Protein Information

nogo-B receptor; ngBR

UniProt Comments for Nus1

Function: Acts as a specific receptor for the N-terminus of Nogo-B, a neural and cardiovascular regulator. Regulates the stability of nascent NPC2, thereby promoting trafficking of LDL-derived cholesterol

By similarity.Essential component of the dolichol monophosphate (Dol-P) biosynthetic machinery

By similarity.

Pathway: Protein modification; protein glycosylation.

Subunit structure: Interacts with DHDDS, promoting its isoprenyltransferase activity. Interacts with NPC2

By similarity.

Subcellular location: Endoplasmic reticulum membrane; Single-pass type I membrane protein. Note: Colocalizes with Nogo-B during VEGF and wound healing angiogenesis

By similarity.

Tissue specificity: Highly expressed in heart, liver, kidney and pancreas. Ref.3

Miscellaneous: Although strongly related to UPP synthase family proteins, it has no lipid transferase activity.NUS1 seems to exist in two topological orientations, a minor glycosylated species with its C-terminus oriented towards the lumen regulating NPC2 stability, and a major fraction oriented with its C-terminus directed towards the cytosol where it regulates cis-IPTase activity

By similarity.

Sequence similarities: Belongs to the UPP synthase family.

Sequence caution: The sequence AAH18372.1 differs from that shown. Reason: Frameshift at position 10. The sequence BAE33349.1 differs from that shown. Reason: Frameshift at position 252.

Research Articles on Nus1

1. Our results indicate that the NgBR ectodomain is intrinsically unstructured without tertiary structures while the cytoplasmic domain is partially folded with secondary structures but without a tight tertiary packing [nogo-b receptor]

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.