Rat Anti-CD4 Monoclonal Antibody – Cat# MBS690383

CD4 Antibody – Rat Monoclonal

Name: CD4 Antibody; Monoclonal; Rat; Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS690383
Price: 875 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS690383

CD4 is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T cells. In humans, CD4 is also expressed to a lesser extent on monocytes and macrophage related cells. More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

CD4, Monoclonal Antibody

Full Product Name

Rat Anti-Mouse CD4

Product Gene Name

anti-CD4 antibody

Product Synonym Names

Anti-mouse CD4 (#2H9)

Clonality

Monoclonal

Isotype

IgG2

Clone Number

(2H9)

Host

Rat

Species Reactivity

Mouse

Form/Format

The antibody was purified from tissue culture supernatant by affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS).

Purity/Purification

Protein A/G affinity chromatography

Preparation and Storage

Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity.

Molecular Weight

51,297 Da

Chromosome Location

Chromosome: 6; NC_000072.6 (124864693..124888246, complement). Location: 6 F2; 6 59.17 cM

3D Structure

ModBase 3D Structure for P06332

Antibody Generation

This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat) immunized with mouse CTL cells. IgG2 fraction of the culture supernatant was purified by Protein G affinity chromatography.

Reconstitution

Reconstitute the antibody with 400 ul sterile PBS and the final concentration is 500 ug/ml.

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-CD4 antibody

Immunohistochemistry (IHC) - Paraffin, Flow Cytometry (FC/FACS)

Application Notes for anti-CD4 antibody

1. Immunohistochemistry (Paraffin): Yes (1:100-300) 2. Flow Cytometry: Yes (1:100-1000)

Other Notes

Small volumes of anti-CD4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD4 purchase

MBS690383 is an antibody targeting CD4. Also known as Rat Anti-Mouse CD4. Reactive in Mouse. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for CD4. It may not necessarily be applicable to this product.

NCBI GI #

7304953

NCBI GeneID

12504

NCBI Accession #

NP_038516.1

UniProt Primary Accession #

P06332

UniProt Related Accession #

P06332

GenBank Nucleotide #

NM_013488.2

NCBI Official Full Name

T-cell surface glycoprotein CD4

NCBI Official Synonym Full Names

CD4 antigen

NCBI Official Symbol

Cd4

NCBI Official Synonym Symbols

L3T4; Ly-4

UniProt Protein Name

T-cell surface glycoprotein CD4

UniProt Synonym Protein Names

T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3

Protein Family

T-cell surface glycoprotein

UniProt Gene Name

Cd4

UniProt Entry Name

CD4_MOUSE

NCBI Protein Information

T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; T-cell differentiation antigen L3T4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4)

UniProt Comments for CD4

Function: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation.

Subunit structure: Associates with p56-LCK. Interacts with SPG21

By similarity.

Subcellular location: Membrane; Single-pass type I membrane protein.

Sequence similarities: Contains 3 Ig-like C2-type (immunoglobulin-like) domains.Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Research Articles on CD4

1. Both type I CD8+ cytotoxic (Tc1) cells and interleukin (IL)-17-producing CD8+ (Tc17) cells mediate effective antitumor immunity through distinct effector mechanisms, but Tc1 cells are superior to Tc17 cells in mediating tumor regression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CD4 antibody

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Alpha-defensins Pathway antibodies	Alpha-defensins Pathway Diagram
Antigen Processing And Presentation Pathway antibodies	Antigen Processing And Presentation Pathway Diagram
Antigen Processing And Presentation Pathway antibodies	Antigen Processing And Presentation Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Costimulation By The CD28 Family Pathway antibodies	Costimulation By The CD28 Family Pathway Diagram
Cytokines And Inflammatory Response (BioCarta) Pathway antibodies	Cytokines And Inflammatory Response (BioCarta) Pathway Diagram
Defensins Pathway antibodies	Defensins Pathway Diagram
Downstream TCR Signaling Pathway antibodies	Downstream TCR Signaling Pathway Diagram

Diseases associated with anti-CD4 antibody

Disease Name	Pubmed Publications
Immune System Diseases Antibodies	>54639 publications with CD4 and Immune System Diseases
Neoplasms Antibodies	>15040 publications with CD4 and Neoplasms
Inflammation Antibodies	>11068 publications with CD4 and Inflammation
Necrosis Antibodies	>7268 publications with CD4 and Necrosis
Lung Diseases Antibodies	>6400 publications with CD4 and Lung Diseases
Liver Diseases Antibodies	>4347 publications with CD4 and Liver Diseases
AIDS-Related Opportunistic Infections Antibodies	>4242 publications with CD4 and AIDS-Related Opportunistic Infections
Carcinoma Antibodies	>3215 publications with CD4 and Carcinoma
Cardiovascular Diseases Antibodies	>2905 publications with CD4 and Cardiovascular Diseases
Neoplasms, Experimental Antibodies	>2845 publications with CD4 and Neoplasms, Experimental

Organs/Tissues associated with anti-CD4 antibody

Organ/Tissue Name	Pubmed Publications
Spleen Antibodies	>9033 publications with CD4 and Spleen
Thymus Antibodies	>7010 publications with CD4 and Thymus
Mixed Antibodies	>3650 publications with CD4 and Mixed
Brain Antibodies	>3091 publications with CD4 and Brain
Salivary Gland Antibodies	>403 publications with CD4 and Salivary Gland
Mammary Gland Antibodies	>133 publications with CD4 and Mammary Gland
Pineal Gland Antibodies	>33 publications with CD4 and Pineal Gland
Uncharacterized Tissue Antibodies	>19 publications with CD4 and Uncharacterized Tissue