Angiotensin-Converting Enzyme (CD143) Antibody – Rat Monoclonal

Function: Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. This GPIase activity seems to be crucial for the egg-binding ability of the sperm. Ref.6 Ref.7 Ref.8

Catalytic activity: Release of a C-terminal dipeptide, oligopeptide-|-Xaa-Yaa, when Xaa is not Pro, and Yaa is neither Asp nor Glu. Thus, conversion of angiotensin I to angiotensin II, with increase in vasoconstrictor activity, but no action on angiotensin II.

Cofactor: Binds 2 zinc ions per subunit. Isoform Testis-specific only binds 1 zinc ion per subunit

By similarity.Binds 3 chloride ions per subunit

By similarity.

Enzyme regulation: Peptidase activity is specifically inhibited by lisinopril, captopril and enalaprilat. In contrast, GPIase activity is nearly insensitive to captopril. Ref.8

Subcellular location: Angiotensin-converting enzyme, soluble form: Secreted

By similarity. Cell membrane; Single-pass type I membrane protein

By similarity.

Tissue specificity: Testis-specific isoform isexpressed in spermatocytes, adult testis.

Induction: Expression is thought to be subject to hormonal regulation by androgens. Ref.8

Post-translational modification: Phosphorylated by CK2 on Ser-1305; which allows membrane retention

By similarity.

Disruption phenotype: Low blood pressure, elevated serum potassium, anemia, and renal defects. Male mice have reduced fertility. Ref.7

Sequence similarities: Belongs to the peptidase M2 family.