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CD238 Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS690492

Kell is a zinc protease of the neprilysin family. Kell forms a complex with XK, a putative membrane transporter, to constitute the Kell blood group antigens. It has been shown to cleave endothelins. More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Product Name
Kell (CD238), Monoclonal Antibody
Full Product Name
Rat Anti-Mouse Kell (CD238)
Product Gene Name
Product Synonym Names
Anti-mouse Kell [blood group protein, CD238] (#2K15)
Clonality
Monoclonal
Isotype
IgG2
Clone Number
(7L32)
Host
Rat
Species Reactivity
Mouse
Form/Format
The IgG fraction of culture supernatant was purified by Protein A/G affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS). Lyophilized samples are stable for 2 years from date of receipt when stored at
Purity/Purification
Protein A/G affinity chromatography
Specificity
This antibody detects mouse Kell (CD238) in Western blotting.
Preparation and Storage
Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity.
Molecular Weight
80,866 Da
Chromosome Location
Chromosome: 6; NC_000072.6 (41686329..41704334, complement). Location: 6 B2.1; 6 19.86 cM
3D Structure
ModBase 3D Structure for Q9EQF2
Antibody Generation
This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse recombinant protein of Kell (CD238) extracellular domain. The IgG2 fraction of the culture supernatant was purified by Protein A/G affinity chromatography.
Reconstitution
Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-CD238 antibody
Western Blot (WB)
Application Notes for anti-CD238 antibody
1. Western blot: Yes (1:500-1000)

Other Notes

Small volumes of anti-CD238 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD238 purchase

MBS690492 is an antibody targeting CD238. Also known as Rat Anti-Mouse Kell (CD238). Reactive in Mouse. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for CD238. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
kell blood group glycoprotein homolog
NCBI Official Synonym Full Names
Kell blood group
NCBI Official Symbol
Kel
NCBI Official Synonym Symbols
Ece3; CD238
UniProt Protein Name
Kell blood group glycoprotein homolog
UniProt Gene Name
Kel
UniProt Entry Name
KELL_MOUSE
NCBI Protein Information
kell blood group glycoprotein homolog; Kell protein; Kel blood group protein; Kell blood group, metallo-endopeptidase

UniProt Comments for CD238

Function: Zinc endopeptidase with endothelin-3-converting enzyme activity. Cleaves EDN1, EDN2 and EDN3

By similarity. Ref.1

Cofactor: Binds 1 zinc ion per subunit

By similarity.

Subunit structure: Heterodimer with XK; disulfide-linked. Ref.1

Subcellular location: Cell membrane; Single-pass type II membrane protein

By similarity. Note: Spans the erythrocyte membrane, and is attached to the underlying cytoskeleton

By similarity.

Tissue specificity: Highly expressed in spleen. Weaker expression in testis and heart. Ref.1

Post-translational modification: N-glycosylated. Ref.1

Sequence similarities: Belongs to the peptidase M13 family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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