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CD40 Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS690527

CD40 plays a key role in B-cell growth and differentiation where interactions of CD40 with its ligand, CD154, are involved in the initiation, effector, and memory stages of cell-mediated immune responses. In addition, CD40 has been reported to be involved… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
CD40, Monoclonal Antibody
Full Product Name
Rat Anti-Mouse CD40
Product Gene Name
Product Synonym Names
Anti mouse CD40; Cd40; IGM; p50; Bp50; GP39; IMD3; TRAP; HIGM1; T-BAM; Tnfrsf5; AI326936
Clonality
Monoclonal
Isotype
IgG2
Clone Number
(MAB0805)
Host
Rat
Species Reactivity
Mouse
Form/Format
Lyophilized
Purity/Purification
Protein G/A chromatography
Specificity
This antibody was selected for its ability to detect mouse CD40
Preparation and Storage
Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least for six months without detectable loss of activity.
Chromosome Location
Chromosome: 2; NC_000068.7 (165055598..165071654). Location: 2 H3; 2 85.38 cM
3D Structure
ModBase 3D Structure for P27512
Preparation
This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with recombinant mouse CD40 protein. IgG2 fraction of the culture supernatant was purified by Protein G affinity chromatography.
Antigen
Mouse recombinant CD40
Reconstitution
Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml.
Reconstitution Buffer
PBS (sterile)
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-CD40 antibody
Immunohistochemistry (IHC) - Paraffin
Application Notes for anti-CD40 antibody
Immunohistochemistry (Paraffin):(1:25-100)

Testing Data of anti-CD40 antibody

Rat Anti-CD40 Monoclonal Antibody – Cat# MBS690527

Other Notes

Small volumes of anti-CD40 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD40 purchase

MBS690527 is an antibody targeting CD40. Also known as Rat Anti-Mouse CD40. Reactive in Mouse. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for CD40. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 5 isoform 1
NCBI Official Synonym Full Names
CD40 antigen
NCBI Official Symbol
Cd40
NCBI Official Synonym Symbols
IGM; p50; Bp50; GP39; IMD3; TRAP; HIGM1; T-BAM; Tnfrsf5; AI326936
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 5
UniProt Synonym Protein Names
B-cell surface antigen CD40; Bp50; CD40L receptor
UniProt Gene Name
Cd40
UniProt Synonym Gene Names
Tnfrsf5
UniProt Entry Name
TNR5_MOUSE
NCBI Protein Information
tumor necrosis factor receptor superfamily member 5; CD40L receptor; B-cell surface antigen CD40; T-cell differentiation antigen; tumor necrosis factor receptor superfamily, member 5

UniProt Comments for CD40

Function: Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion. Ref.11

Subunit structure: Monomer and homodimer. Interacts with TRAF1, TRAF2 and TRAF6

By similarity. Interacts with TRAF3 and TRAF5. Interacts with TRAF6 and MAP3K8; the interaction is required for ERK activation. Ref.9 Ref.10 Ref.11

Subcellular location: Isoform I: Cell membrane; Single-pass type I membrane protein. Isoform III: Cell membrane; Single-pass type I membrane protein. Isoform IV: Cell membrane; Single-pass type I membrane protein. Isoform V: Cell membrane; Single-pass type I membrane protein. Isoform II: Secreted.

Sequence similarities: Contains 4 TNFR-Cys repeats.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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