Rat Anti-ICAM-1 Monoclonal Antibody – Cat# MBS690600

ICAM-1 Antibody – Rat Monoclonal

Name: ICAM-1 Antibody; Monoclonal; Rat; Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS690600
Price: 875 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS690600

Intercellular adhesion molecule 1 (ICAM-1), also known as CD54, binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

ICAM-1, Monoclonal Antibody

Full Product Name

Rat Anti-Mouse ICAM-1

Product Gene Name

anti-ICAM-1 antibody

Product Synonym Names

Anti-mouse ICAM-1

Clonality

Monoclonal

Isotype

IgG2

Clone Number

(MAB0802)

Host

Rat

Species Reactivity

Mouse

Form/Format

Lyophilized

Purity/Purification

Protein A/G affinity chromatography

Preparation and Storage

Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity.

Molecular Weight

58,844 Da

Chromosome Location

Chromosome: 9; NC_000075.6 (21015960..21028797). Location: 9 A3; 9 7.69 cM

3D Structure

ModBase 3D Structure for P13597

Antibody Generation

This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with cytokine stimulated mouse endothelial cells. IgG fraction of the culture supernatant was purified by Protein A/G affinity chromatography. ICAM-1, a 95 kDa surface antigen found on endothelial cells, lymphocytes,epithelial cells, dendritic cells, and keratinocytes. ICAM-1 is a ligand for LFA-1 (CD11a/CD18) and MAC-1(CD11b/CD18). Its expression is up regulated upon stimulation by inflammatory mediators such as cytokines and LPS and it's involved in B cell T-cell co- stimulatory interactions.

Reconstitution

Reconstitute the antibody with 400 ul sterile PBS and the final concentration is 500 ug/ml.

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-ICAM-1 antibody

Immunohistochemistry (IHC) - Paraffin, Flow Cytometry (FC/FACS)

Application Notes for anti-ICAM-1 antibody

1. Flow Cytometery: Yes (1:100-1000) 2. Immunohistochemistry (Paraffin): Yes (1:500-2000)

Other Notes

Small volumes of anti-ICAM-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ICAM-1 purchase

MBS690600 is an antibody targeting ICAM-1. Also known as Rat Anti-Mouse ICAM-1. Reactive in Mouse. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for ICAM-1. It may not necessarily be applicable to this product.

NCBI GI #

21389311

NCBI GeneID

15894

NCBI Accession #

NP_034623.1

UniProt Primary Accession #

P13597

UniProt Secondary Accession #

Q61828

UniProt Related Accession #

P13597

GenBank Nucleotide #

NM_010493.2

NCBI Official Full Name

intercellular adhesion molecule 1

NCBI Official Synonym Full Names

intercellular adhesion molecule 1

NCBI Official Symbol

Icam1

NCBI Official Synonym Symbols

CD54; Ly-47; Icam-1; MALA-2

UniProt Protein Name

Intercellular adhesion molecule 1

UniProt Synonym Protein Names

MALA-2; MyD10

Protein Family

Intercellular adhesion molecule

UniProt Gene Name

Icam1

UniProt Synonym Gene Names

Icam-1; ICAM-1

UniProt Entry Name

ICAM1_MOUSE

NCBI Protein Information

intercellular adhesion molecule 1; myD10

UniProt Comments for ICAM-1

Function: ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation

By similarity.

Subunit structure: Homodimer. Interacts with MUC1 and promotes cell aggregation in epithelial cells. Interacts with ARHGEF26/SGEF

By similarity.

Subcellular location: Membrane; Single-pass type I membrane protein.

Tissue specificity: Expressed at low level on a subpopulation of lymphocytes, macrophages, and endothelial cells, but is strongly induced on these cells, and on fibroblasts and epithelial cells.

Post-translational modification: Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis

By similarity.

Sequence similarities: Belongs to the immunoglobulin superfamily. ICAM family.Contains 5 Ig-like C2-type (immunoglobulin-like) domains.

Research Articles on ICAM-1

1. expression of E-selectin and ICAM-1 are suppressed by paeoniflorin in a mouse model of cutaneous Arthus reaction

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ICAM-1 antibody

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
African Trypanosomiasis Pathway antibodies	African Trypanosomiasis Pathway Diagram
African Trypanosomiasis Pathway antibodies	African Trypanosomiasis Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cytokine Signaling In Immune System Pathway antibodies	Cytokine Signaling In Immune System Pathway Diagram
Epstein-Barr Virus Infection Pathway antibodies	Epstein-Barr Virus Infection Pathway Diagram
Epstein-Barr Virus Infection Pathway antibodies	Epstein-Barr Virus Infection Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram
HTLV-I Infection Pathway antibodies	HTLV-I Infection Pathway Diagram

Diseases associated with anti-ICAM-1 antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>5303 publications with ICAM-1 and Inflammation
Necrosis Antibodies	>4587 publications with ICAM-1 and Necrosis
Cardiovascular Diseases Antibodies	>3425 publications with ICAM-1 and Cardiovascular Diseases
Atherosclerosis Antibodies	>1371 publications with ICAM-1 and Atherosclerosis
Reperfusion Injury Antibodies	>838 publications with ICAM-1 and Reperfusion Injury
Myocardial Ischemia Antibodies	>766 publications with ICAM-1 and Myocardial Ischemia
Kidney Diseases Antibodies	>729 publications with ICAM-1 and Kidney Diseases
Liver Diseases Antibodies	>527 publications with ICAM-1 and Liver Diseases
Hypertension Antibodies	>496 publications with ICAM-1 and Hypertension
Asthma Antibodies	>484 publications with ICAM-1 and Asthma

Organs/Tissues associated with anti-ICAM-1 antibody

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>1681 publications with ICAM-1 and Lung
Skin Antibodies	>1181 publications with ICAM-1 and Skin
Liver Antibodies	>1136 publications with ICAM-1 and Liver
Brain Antibodies	>1127 publications with ICAM-1 and Brain
Kidney Antibodies	>1126 publications with ICAM-1 and Kidney
Embryonic Tissue Antibodies	>763 publications with ICAM-1 and Embryonic Tissue
Bone Antibodies	>627 publications with ICAM-1 and Bone
Intestine Antibodies	>523 publications with ICAM-1 and Intestine
Bone Marrow Antibodies	>462 publications with ICAM-1 and Bone Marrow
Lymph Node Antibodies	>400 publications with ICAM-1 and Lymph Node