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CD8a Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS690602

CD8 is a heterodimeric glycoprotein consisting of an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC/peptide complexes. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
CD8a, Monoclonal Antibody
Full Product Name
Rat Anti-Mouse CD8a
Product Gene Name
Product Synonym Names
Anti mouse CD8a (#10F14)
Clonality
Monoclonal
Isotype
IgG2
Clone Number
(MAB0708)
Host
Rat
Species Reactivity
Mouse
Form/Format
The antibody was purified from tissue culture supernatant by affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS).
Purity/Purification
Protein A/G affinity chromatography
Preparation and Storage
Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity.
Molecular Weight
27,456 Da
Chromosome Location
Chromosome: 6; NC_000072.6 (71352782..71379171). Location: 6 C; 6 32.14 cM
3D Structure
ModBase 3D Structure for P01731
Antibody Generation
This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with mouse Thymus/Spleen Tissues. IgG2 fraction of the culture supernatant was purified by Protein G affinity chromatography.
Reconstitution
Reconstitute the antibody with 400 ul sterile PBS and the final concentration is 500 ug/ml.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-CD8a antibody
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-CD8a antibody
1. Immunohistochemistry (Paraffin): Yes (1:100-300) 2. Flow Cytometery: Yes (1:100-1000)

Testing Data of anti-CD8a antibody

Rat Anti-CD8a Monoclonal Antibody – Cat# MBS690602

Other Notes

Small volumes of anti-CD8a antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD8a purchase

MBS690602 is an antibody targeting CD8a. Also known as Rat Anti-Mouse CD8a. Reactive in Mouse. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for CD8a. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
T-cell surface glycoprotein CD8 alpha chain isoform 1
NCBI Official Synonym Full Names
CD8 antigen, alpha chain
NCBI Official Symbol
Cd8a
NCBI Official Synonym Symbols
Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
UniProt Protein Name
T-cell surface glycoprotein CD8 alpha chain
UniProt Synonym Protein Names
T-cell surface glycoprotein Lyt-2
UniProt Gene Name
Cd8a
UniProt Synonym Gene Names
Lyt-2; Lyt2
UniProt Entry Name
CD8A_MOUSE
NCBI Protein Information
T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein Lyt-2; Lyt-2.1 lymphocyte differentiation antigen (AA at 100)

UniProt Comments for CD8a

Function: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Subunit structure: In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Ref.7

Subcellular location: Membrane; Single-pass type I membrane protein.

Sequence similarities: Contains 1 Ig-like V-type (immunoglobulin-like) domain.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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