CD147 Antibody – Rat Monoclonal

Function: Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes. Ref.9 Ref.11

Subunit structure: Forms homooligomers in a cis-dependent manner on the plasma membrane. Forms a complex with MMP1 at the tumor cell surface

By similarity. Interacts with SLC16A1 and SLC1A3; probably a BSG dimer is associated with a monocarboxylate transporter dimer. Interacts with AJAP1

By similarity. Interacts with ATP1B2, MAG and L1CAM. Ref.9

Subcellular location: Cell membrane; Single-pass type I membrane protein. Melanosome

By similarity. Note: Colocalizes with SLC16A1 and SLC16A8. In spermatozoa, localized on the principal piece of caput and in the middle piece during transit in the corpus and cauda epididymides. Ref.11 Ref.13

Tissue specificity: Isoform 1 is specifically expressed in retina. Isoform 2 is widely expressed, including adult organs, embryos and EC cells. Expressed in spermatozoa. Ref.6

Developmental stage: In developing eye expressed at embryonic days E12, E15 and E18, and at postnatal days P1, P7, P14, and P21. Expression progressed from a more generalized distribution throughout the undifferentiated neural retina to specific staining of retina-pigmented epithilia, the MCs, photoreceptor cells and the ciliary apparatus. Expression is highest at P21. Isoform 1 and isoform 2 are expressed at equivalent levels at P7, isoform 1 is more abundant at P14, P21 and P28. In uterus during the peri-implantation period strongly expressed in luminal and glandular epithelium on day 1 of pregnancy and gradually decreased to a basal level from day 2-4 of pregnancy. Expression in the sub-luminal stroma was first detected on day 3 of pregnancy and increased on day 4 of pregnancy. On day 5 of pregnancy, the expression of basigin protein and mRNA was only detected in the implanting embryos, and the luminal epithelium and sub-luminal stroma surrounding the embryos. In ovary during sexual maturation expressed in the granulosa cells of preantral follicles at days 20 and 25 after birth. Expressed during corpus luteum formation. Ref.12 Ref.14 Ref.15

Induction: By estrogen in the uterine epithelium of ovariectomized animals. By eCG in ovary. Ref.12 Ref.15

Post-translational modification: N-glycosylated. During spermatogenesis, probably deglycosylated during epididymal transit. Ref.9 Ref.13

Sequence similarities: Contains 1 Ig-like C2-type (immunoglobulin-like) domain.Contains 1 Ig-like V-type (immunoglobulin-like) domain.