Mouse Anti-FAS Monoclonal Antibody – Cat# MBS690828

FAS Antibody – Mouse Monoclonal

Name: FAS Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS690828
Price: 875 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS690828

Fas, also known as APO1, CD95, and TNFRSF6, was originally identified as a cellsurface protein which binds to monoclonal antibodies that were cytolytic for various human cell lines. In the new TNF Receptor superfamily nomenclature, Fas is referred to as… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

FAS, Monoclonal Antibody

Full Product Name

Mouse Anti-Human FAS

Product Gene Name

anti-FAS antibody

Product Synonym Names

Anti human FAS (#12J3)

Clonality

Monoclonal

Isotype

IgG1

Clone Number

(12J3)

Host

Mouse

Species Reactivity

Human

Form/Format

Lyophilized

Purity/Purification

Protein G affinity chromatography

Specificity

This antibody recognizes human Fas in western blot.

Preparation and Storage

Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity.

Molecular Weight

37,437 Da

Chromosome Location

Chromosome: 19; NC_000085.6 (34290659..34327770). Location: 19 C1; 19 29.48 cM

3D Structure

ModBase 3D Structure for P25446

Antibody Generation

This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with recombinant human Fas protein.The IgG1 fraction of the tissue culture supernatant was purified by Protein G affinity chromatography.

Reconstitution

Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml.

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-FAS antibody

Western Blot (WB), Immunohistochemistry (IHC) - Paraffin

Application Notes for anti-FAS antibody

1. Western blotting: Yes (1:100-1000) 2. Immunohistochemistry (Paraffin): Yes (1:50-200)

Other Notes

Small volumes of anti-FAS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FAS purchase

MBS690828 is an antibody targeting FAS. Also known as Mouse Anti-Human FAS. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for FAS. It may not necessarily be applicable to this product.

NCBI GI #

226443049

NCBI GeneID

14102

NCBI Accession #

NP_032013.2

UniProt Primary Accession #

P25446

UniProt Secondary Accession #

Q6GT31; Q9DCQ1

UniProt Related Accession #

P25446

GenBank Nucleotide #

NM_007987.2

NCBI Official Full Name

tumor necrosis factor receptor superfamily member 6 isoform 1

NCBI Official Synonym Full Names

Fas (TNF receptor superfamily member 6)

NCBI Official Symbol

Fas

NCBI Official Synonym Symbols

lpr; APO1; APT1; CD95; TNFR6; Tnfrsf6; AI196731

UniProt Protein Name

Tumor necrosis factor receptor superfamily member 6

UniProt Synonym Protein Names

Apo-1 antigen; Apoptosis-mediating surface antigen FAS; FASLG receptor

Protein Family

Tumor necrosis factor receptor superfamily

UniProt Gene Name

Fas

UniProt Synonym Gene Names

Apt1; Tnfrsf6

UniProt Entry Name

TNR6_MOUSE

NCBI Protein Information

tumor necrosis factor receptor superfamily member 6; Fas antigen; apo-1 antigen; FASLG receptor; lymphoproliferation; Fas (TNF receptor superfamily member); apoptosis-mediating surface antigen FAS

UniProt Comments for FAS

Function: Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both

By similarity.

Subunit structure: Binds DAXX and RIPK1. Interacts with BRE and FEM1B

By similarity. Interacts with HIPK3. Part of a complex containing HIPK3 and FADD. Interacts with FADD

By similarity. Ref.8 Ref.9

Subcellular location: Membrane; Single-pass type I membrane protein.

Tissue specificity: Detected in various tissues including thymus, liver, lung, heart, and adult ovary.

Domain: Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.

Involvement in disease: Defects in Fas are the cause of the lymphoproliferation phenotype (lpr). Lpr mice show lymphadenopathy and autoantibody production.

Sequence similarities: Contains 1 death domain.Contains 3 TNFR-Cys repeats.

Research Articles on FAS

1. Fas signal from activated T cells promotes the immunosuppressive function of Fas-expressing regulatory DCs, providing a new manner for the regulatory DCs to regulate adaptive immunity

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-FAS antibody

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Adipogenesis Pathway antibodies	Adipogenesis Pathway Diagram
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Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Alzheimer's Disease Pathway antibodies	Alzheimer's Disease Pathway Diagram
Alzheimer's Disease Pathway antibodies	Alzheimer's Disease Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram

Diseases associated with anti-FAS antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>5511 publications with FAS and Neoplasms
Necrosis Antibodies	>4856 publications with FAS and Necrosis
Autoimmune Diseases Antibodies	>1943 publications with FAS and Autoimmune Diseases
Inflammation Antibodies	>1625 publications with FAS and Inflammation
Nervous System Diseases Antibodies	>1468 publications with FAS and Nervous System Diseases
Disease Models, Animal Antibodies	>1363 publications with FAS and Disease Models, Animal
Liver Diseases Antibodies	>1324 publications with FAS and Liver Diseases
Cardiovascular Diseases Antibodies	>1218 publications with FAS and Cardiovascular Diseases
Fatty Liver Antibodies	>877 publications with FAS and Fatty Liver
Hematologic Diseases Antibodies	>639 publications with FAS and Hematologic Diseases

Organs/Tissues associated with anti-FAS antibody

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>2737 publications with FAS and Liver
Lung Antibodies	>1148 publications with FAS and Lung
Kidney Antibodies	>823 publications with FAS and Kidney
Bone Marrow Antibodies	>791 publications with FAS and Bone Marrow
Spleen Antibodies	>739 publications with FAS and Spleen
Eye Antibodies	>494 publications with FAS and Eye
Connective Tissue Antibodies	>481 publications with FAS and Connective Tissue
Joint Antibodies	>438 publications with FAS and Joint
Intestine Antibodies	>387 publications with FAS and Intestine
Pancreas Antibodies	>360 publications with FAS and Pancreas