Rat Anti-WIF-1 Monoclonal Antibody – Cat# MBS690879

WIF-1 Antibody – Rat Monoclonal

Name: WIF-1 Antibody; Monoclonal; Rat; Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS690879
Price: 875 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS690879

WIF-1 (Wnt Inhibitory Factor 1) is a secreted protein that binds to Wnt proteins and inhibits their activity. In situ hybridization analysis in Xenopus laevis and zebrafish indicate that the message is highly expressed in presomitic mesoderm, the notochord,… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

WIF-1 (Wnt inhibitory factor 1), Monoclonal Antibody

Full Product Name

Rat Anti-Mouse WIF-1 (Wnt inhibitory factor 1)

Product Gene Name

anti-WIF-1 antibody

Product Synonym Names

Anti-mouse Wnt inhibitory factor 1 [WIF-1] (#3M51)

Clonality

Monoclonal

Isotype

IgG2

Clone Number

(3M51)

Host

Rat

Species Reactivity

Mouse

Form/Format

The IgG fraction of culture supernatant was purified by Protein A/G affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS). Lyophilized samples are stable for 2 years from date of receipt when stored at

Purity/Purification

Protein A/G affinity chromatography

Specificity

This antibody detects mouse of WIF-1 in Western blotting.

Preparation and Storage

Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity.

Molecular Weight

41,590 Da

Chromosome Location

Chromosome: 10; NC_000076.6 (121033941..121100642). Location: 10 D2; 10

3D Structure

ModBase 3D Structure for Q9WUA1

Antibody Generation

This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse recombinant WIF-1. The IgG2 fraction of the culture supernatant was purified by Protein A/G affinity chromatography.

Reconstitution

Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml.

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Application Notes for anti-WIF-1 antibody

1. Western blotting: Yes (1:500-1000)

Other Notes

Small volumes of anti-WIF-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for WIF-1 purchase

MBS690879 is an antibody targeting WIF-1. Also known as Rat Anti-Mouse WIF-1 (Wnt inhibitory factor 1). Reactive in Mouse. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for WIF-1. It may not necessarily be applicable to this product.

NCBI GI #

6755999

NCBI GeneID

24117

NCBI Accession #

NP_036045.1

UniProt Primary Accession #

Q9WUA1

UniProt Related Accession #

Q9WUA1

GenBank Nucleotide #

NM_011915.2

NCBI Official Full Name

wnt inhibitory factor 1

NCBI Official Synonym Full Names

Wnt inhibitory factor 1

NCBI Official Symbol

Wif1

NCBI Official Synonym Symbols

WIF-1; AW107799

UniProt Protein Name

Wnt inhibitory factor 1

UniProt Gene Name

Wif1

UniProt Synonym Gene Names

WIF-1

UniProt Entry Name

WIF1_MOUSE

NCBI Protein Information

wnt inhibitory factor 1

UniProt Comments for WIF-1

Function: Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation.

Subcellular location: Secreted.

Tissue specificity: Expression highest in heart and lung. Lower in brain and eye.

Sequence similarities: Contains 5 EGF-like domains.Contains 1 WIF domain.

Research Articles on WIF-1

1. These data suggest that WIF-1 may take part in the fine-tuning of cartilage and bone turnover, promoting the balance of cartilage versus bone anabolism.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-WIF-1 antibody

Reference Product	PubMed Publications
Wnt5a antibody	>14 publications with WIF-1 and Wnt5a
Wnt1 antibody	>8 publications with WIF-1 and Wnt1
Wnt3a antibody	>6 publications with WIF-1 and Wnt3a
Wnt11 antibody	>4 publications with WIF-1 and Wnt11
Wnt10b antibody	>3 publications with WIF-1 and Wnt10b
Wnt7a antibody	>2 publications with WIF-1 and Wnt7a
Wnt7b antibody	>2 publications with WIF-1 and Wnt7b
Wnt2b antibody	>1 publications with WIF-1 and Wnt2b

Pathways associated with anti-WIF-1 antibody

Products by Pathway	Pathway Diagram
PodNet: Protein-protein Interactions In The Podocyte Pathway antibodies	PodNet: Protein-protein Interactions In The Podocyte Pathway Diagram
Wnt Signaling Pathway antibodies	Wnt Signaling Pathway Diagram
Wnt Signaling Pathway antibodies	Wnt Signaling Pathway Diagram
XPodNet - Protein-protein Interactions In The Podocyte Expanded By STRING Pathway antibodies	XPodNet - Protein-protein Interactions In The Podocyte Expanded By STRING Pathway Diagram

Diseases associated with anti-WIF-1 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>121 publications with WIF-1 and Neoplasms
Carcinoma Antibodies	>53 publications with WIF-1 and Carcinoma
Skin Diseases Antibodies	>21 publications with WIF-1 and Skin Diseases
Lung Neoplasms Antibodies	>20 publications with WIF-1 and Lung Neoplasms
Lung Diseases Antibodies	>19 publications with WIF-1 and Lung Diseases
Adenocarcinoma Antibodies	>18 publications with WIF-1 and Adenocarcinoma
Colorectal Neoplasms Antibodies	>18 publications with WIF-1 and Colorectal Neoplasms
Neoplasms, Experimental Antibodies	>10 publications with WIF-1 and Neoplasms, Experimental
Breast Neoplasms Antibodies	>10 publications with WIF-1 and Breast Neoplasms
Carcinoma, Hepatocellular Antibodies	>7 publications with WIF-1 and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-WIF-1 antibody

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>34 publications with WIF-1 and Bone
Lung Antibodies	>27 publications with WIF-1 and Lung
Eye Antibodies	>14 publications with WIF-1 and Eye
Brain Antibodies	>13 publications with WIF-1 and Brain
Skin Antibodies	>13 publications with WIF-1 and Skin
Embryonic Tissue Antibodies	>11 publications with WIF-1 and Embryonic Tissue
Bladder Antibodies	>7 publications with WIF-1 and Bladder
Heart Antibodies	>5 publications with WIF-1 and Heart
Stomach Antibodies	>4 publications with WIF-1 and Stomach
Joint Antibodies	>3 publications with WIF-1 and Joint