Rat Anti-CTLA-4 Monoclonal Antibody – Cat# MBS690911

CTLA-4 Antibody – Rat Monoclonal

Name: CTLA-4 Antibody; Monoclonal; Rat; Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS690911
Price: 875 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS690911

CD28 and CTLA-4, together with their ligands, B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

CTLA-4, Monoclonal Antibody

Full Product Name

Rat Anti-Mouse CTLA-4

Product Gene Name

anti-CTLA-4 antibody

Product Synonym Names

Ctla4; Cd152; Ly-56; Ctla-4

Clonality

Monoclonal

Isotype

IgG2

Clone Number

(6G10)

Host

Rat

Species Reactivity

Mouse

Form/Format

Lyophilized

Preparation and Storage

Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity.

Molecular Weight

24,993 Da

Chromosome Location

Chromosome: 1; NC_000067.6 (60909025..60915832). Location: 1 C; 1 30.58 cM

3D Structure

ModBase 3D Structure for P09793

Antibody Generation

This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat) immunized with mouse recombinant protein of CTLA-4.

Reconstitution

Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml.

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-CTLA-4 antibody

Western Blot (WB), Flow Cytometry (FC/FACS), Neutr

Other Notes

Small volumes of anti-CTLA-4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CTLA-4 purchase

MBS690911 is an antibody targeting CTLA-4. Also known as Rat Anti-Mouse CTLA-4. Reactive in Mouse. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for CTLA-4. It may not necessarily be applicable to this product.

NCBI GI #

31981847

NCBI GeneID

12477

NCBI Accession #

NP_033973.2

UniProt Primary Accession #

P09793

UniProt Secondary Accession #

Q9QZZ7

UniProt Related Accession #

P09793

GenBank Nucleotide #

NM_009843.4

NCBI Official Full Name

cytotoxic T-lymphocyte protein 4 isoform 1

NCBI Official Synonym Full Names

cytotoxic T-lymphocyte-associated protein 4

NCBI Official Symbol

Ctla4

NCBI Official Synonym Symbols

Cd152; Ly-56; Ctla-4

UniProt Protein Name

Cytotoxic T-lymphocyte protein 4

UniProt Synonym Protein Names

Cytotoxic T-lymphocyte-associated antigen 4

Protein Family

Cytotoxic T-lymphocyte protein

UniProt Gene Name

Ctla4

UniProt Synonym Gene Names

Cd152; CTLA-4

UniProt Entry Name

CTLA4_MOUSE

NCBI Protein Information

cytotoxic T-lymphocyte protein 4; CD152 antigen; cytotoxic T-lymphocyte-associated antigen 4

NCBI Summary for CTLA-4

This gene is a member of the immunoglobulin superfamily, and encodes a protein that functions as a negative regulator of T-cell responses. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2013]

UniProt Comments for CTLA-4

Function: Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28

By similarity.

Subunit structure: Homodimer; disulfide-linked. Binds to CD80/B7-1 and CD86/B7.2

By similarity.

Subcellular location: Cell membrane; Single-pass type I membrane protein

By similarity.

Tissue specificity: Widely expressed with highest levels in lymphoid tissues. Ref.2

Post-translational modification: N-glycosylation is important for dimerization

By similarity.Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface

By similarity.

Sequence similarities: Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Research Articles on CTLA-4

1. SOCS3 interacts with CTLA-4 and negatively regulates CTLA-4 levels in T cells, providing a mechanistic explanation for the expansion of regulatory T cells in CD4-SOCS3 during experimental autoimmune uveitis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CTLA-4 antibody

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Autoimmune Thyroid Disease Pathway antibodies	Autoimmune Thyroid Disease Pathway Diagram
Autoimmune Thyroid Disease Pathway antibodies	Autoimmune Thyroid Disease Pathway Diagram
CTLA4 Inhibitory Signaling Pathway antibodies	CTLA4 Inhibitory Signaling Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Costimulation By The CD28 Family Pathway antibodies	Costimulation By The CD28 Family Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Rheumatoid Arthritis Pathway antibodies	Rheumatoid Arthritis Pathway Diagram
Rheumatoid Arthritis Pathway antibodies	Rheumatoid Arthritis Pathway Diagram

Diseases associated with anti-CTLA-4 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>872 publications with CTLA-4 and Neoplasms
Melanoma Antibodies	>435 publications with CTLA-4 and Melanoma
Necrosis Antibodies	>352 publications with CTLA-4 and Necrosis
Inflammation Antibodies	>341 publications with CTLA-4 and Inflammation
Arthritis, Rheumatoid Antibodies	>212 publications with CTLA-4 and Arthritis, Rheumatoid
Graves Disease Antibodies	>170 publications with CTLA-4 and Graves Disease
Carcinoma Antibodies	>162 publications with CTLA-4 and Carcinoma
Liver Diseases Antibodies	>138 publications with CTLA-4 and Liver Diseases
Neoplasms, Experimental Antibodies	>138 publications with CTLA-4 and Neoplasms, Experimental
Lymphoma Antibodies	>80 publications with CTLA-4 and Lymphoma

Organs/Tissues associated with anti-CTLA-4 antibody

Organ/Tissue Name	Pubmed Publications
Spleen Antibodies	>265 publications with CTLA-4 and Spleen
Mixed Antibodies	>232 publications with CTLA-4 and Mixed
Bone Marrow Antibodies	>159 publications with CTLA-4 and Bone Marrow
Thymus Antibodies	>128 publications with CTLA-4 and Thymus
Mammary Gland Antibodies	>1 publications with CTLA-4 and Mammary Gland