Desert Hedgehog (N-Terminal) (DHH) Antibody – Rat Monoclonal

Function: Intercellular signal essential for a variety of patterning events during development. May function as a spermatocyte survival factor in the testes. Essential for testes development.

Subunit structure: Interacts with BOC and CDON. Interacts with HHIP

By similarity.

Subcellular location: Desert hedgehog protein N-product: Cell membrane; Lipid-anchor; Extracellular side

By similarity. Note: The N-terminal peptide remains associated with the cell surface

By similarity.Desert hedgehog protein C-product: Secreted › extracellular space

By similarity. Note: The C-terminal peptide diffuses from the cell

By similarity.

Tissue specificity: Expressed in adult testes. Not expressed in limb buds.

Domain: The desert hedgehog protein N-product binds calcium and zinc ions; this stabilizes the protein fold and is essential for protein-protein interactions mediated by this domain

By similarity.

Post-translational modification: The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). This covalent modification appears to play an essential role in restricting the spatial distribution of the protein activity to the cell surface. The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity

By similarity.

Sequence similarities: Belongs to the hedgehog family.