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IL-10 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS691031

Interleukin 10 (IL-10), initially designated cytokine synthesis inhibitory factor (CSIF), was originally identified as a product of mouse T helper (Th) 2 clones that inhibited cytokine production by Th1 clones. The human ortholog of mouse IL-10 was… More details

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Product Overview

Product Name
IL-10, Monoclonal Antibody
Full Product Name
Mouse Anti-Human IL-10
Product Gene Name
Product Synonym Names
IL10; CSIF; TGIF; GVHDS; IL-10; IL10A
Clonality
Monoclonal
Isotype
IgG2
Clone Number
(6Q24)
Host
Mouse
Species Reactivity
Human
Form/Format
Lyophilized
Preparation and Storage
Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity.
Molecular Weight
20,517 Da
Chromosome Location
Chromosome: 1; NC_000001.11 (206767603..206772494, complement). Location: 1q31-q32
OMIM
124092
3D Structure
ModBase 3D Structure for P22301
Antibody Generation
This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse) immunized with human recombinant protein of IL-10.
Reconstitution
Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-IL-10 antibody
Western Blot (WB), Neutr

Other Notes

Small volumes of anti-IL-10 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL-10 purchase

MBS691031 is an antibody targeting IL-10. Also known as Mouse Anti-Human IL-10. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for IL-10. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
interleukin-10
NCBI Official Synonym Full Names
interleukin 10
NCBI Official Symbol
IL10
NCBI Official Synonym Symbols
CSIF; TGIF; GVHDS; IL-10; IL10A
UniProt Protein Name
Interleukin-10
UniProt Synonym Protein Names
Cytokine synthesis inhibitory factor
UniProt Gene Name
IL10
UniProt Synonym Gene Names
IL-10; CSIF
UniProt Entry Name
IL10_HUMAN
NCBI Protein Information
interleukin-10; T-cell growth inhibitory factor; cytokine synthesis inhibitory factor

NCBI Summary for IL-10

The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011]
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UniProt Comments for IL-10

Function: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.

Subunit structure: Homodimer.

Subcellular location: Secreted.

Tissue specificity: Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.

Polymorphism: A polymorphism in IL10 promoter region is associated with resistance to graft-versus-host disease (GVHDS) [

MIM:614395]. GVHDS is a major complication of allogeneic bone marrow transplantation, in which mature donor T-cells that contaminate the allogeneic bone marrow recognize the tissues of the recipient as foreign, causing a severe inflammatory disease.

Sequence similarities: Belongs to the IL-10 family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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