• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Leptin (LEP) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS691156

Leptin is a product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of leptin are obese and diabetic, and have reduced activity, metabolism, and body temperature. Rat leptin shares approximately 96% and 82% sequence… More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Product Name
Leptin, Polyclonal Antibody
Full Product Name
Rabbit Anti-Human Leptin
Product Gene Name
Product Synonym Names
Antigen-Affinity Purified Polyclonal Antibodies Anti-human Leptin
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Lyophilized
Purity/Purification
Affinity chromatography
Molecular Weight
18,641 Da
Chromosome Location
Chromosome: 7; NC_000007.14 (128241189..128257629). Location: 7q31.3
OMIM
164160
3D Structure
ModBase 3D Structure for P41159
Antibody Generation
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant human Leptin. Anti-human Leptin specific antibody was purified by affinity chromatography employing immobilized human Leptin matrix.
Buffer
PBS; pH 7.2
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-LEP antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-LEP antibody
Western Blot: To detect human Leptin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 g/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human Leptin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Other Notes

Small volumes of anti-LEP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LEP purchase

MBS691156 is an antibody targeting LEP. Also known as Rabbit Anti-Human Leptin. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for LEP. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
leptin
NCBI Official Synonym Full Names
leptin
NCBI Official Symbol
LEP
NCBI Official Synonym Symbols
OB; OBS; LEPD
UniProt Protein Name
Leptin
UniProt Synonym Protein Names
Obese protein; Obesity factor
UniProt Gene Name
LEP
UniProt Synonym Gene Names
OB; OBS
UniProt Entry Name
LEP_HUMAN
NCBI Protein Information
leptin; obese protein; obesity factor; obese, mouse, homolog of; leptin (murine obesity homolog); leptin (obesity homolog, mouse)

NCBI Summary for LEP

This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Jul 2008]
Show more

UniProt Comments for LEP

Function: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.

Subunit structure: Interacts with SIGLEC6. Ref.11

Subcellular location: Secreted.

Involvement in disease: Leptin deficiency (LEPD) [MIM:614962]: A rare disease characterized by low levels of serum leptin, severe hyperphagia and intractable obesity from an early age.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.16

Sequence similarities: Belongs to the leptin family.
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000