Rabbit Anti-M-CSF Polyclonal Antibody – Cat# MBS691203

M-CSF Antibody – Rabbit Polyclonal

Name: M-CSF Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS691203
Price: 605 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS691203

M-CSF, also known as CSF1, is a four-alpha-helicalbundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. M-CSF is also essential for the survival and proliferation of osteoclast progenitors. M-CSF-also primes… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

M-CSF, Polyclonal Antibody

Full Product Name

Rabbit Anti-Human M-CSF

Product Gene Name

anti-M-CSF antibody

Product Synonym Names

Antigen-Affinity Purified Polyclonal Antibodies Anti-human M-CSF

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human

Form/Format

Lyophilized

Purity/Purification

Affinity chromatography

Molecular Weight

60,179 Da

Chromosome Location

Chromosome: 1; NC_000001.11 (109910611..109930994). Location: 1p13.3

OMIM

120420

3D Structure

ModBase 3D Structure for P09603

Antibody Generation

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant human M CSF (human M-CSF). Anti-human M CSF specific antibody was purified by affinity chromatography employing immobilized human M CSF matrix.

Buffer

PBS; pH 7.2

Neutralization

To yield one-half maximal inhibition [ND50] of the biological activity of human M-CSF (7.0 ng/ml), a concentration of 0.02 - 0.03 g/ml of this antibody is required.

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-M-CSF antibody

ELISA (EIA), Western Blot (WB), Neutr

Application Notes for anti-M-CSF antibody

Western Blot: To detect human M-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 g/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human M-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Other Notes

Small volumes of anti-M-CSF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for M-CSF purchase

MBS691203 is an antibody targeting M-CSF. Also known as Rabbit Anti-Human M-CSF. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for M-CSF. It may not necessarily be applicable to this product.

NCBI GI #

27262661

NCBI GeneID

1435

NCBI Accession #

NP_000748.3

UniProt Primary Accession #

P09603

UniProt Secondary Accession #

Q13130; Q14086; Q14806; Q5VVF3; Q5VVF4; Q9UQR8; A8K6J5

UniProt Related Accession #

P09603

GenBank Nucleotide #

NM_000757.5

NCBI Official Full Name

macrophage colony-stimulating factor 1 isoform a

NCBI Official Synonym Full Names

colony stimulating factor 1 (macrophage)

NCBI Official Symbol

CSF1

NCBI Official Synonym Symbols

MCSF; CSF-1

UniProt Protein Name

Macrophage colony-stimulating factor 1

UniProt Synonym Protein Names

Lanimostim

Protein Family

Macrophage colony-stimulating factor 1 receptor

UniProt Gene Name

CSF1

UniProt Synonym Gene Names

CSF-1; M-CSF; MCSF

UniProt Entry Name

CSF1_HUMAN

NCBI Protein Information

macrophage colony-stimulating factor 1; lanimostim

NCBI Summary for M-CSF

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]

UniProt Comments for M-CSF

Function: Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. Ref.19 Ref.21 Ref.22 Ref.23

Subunit structure: Homodimer or heterodimer; disulfide-linked. Interacts with CSF1R. Ref.15 Ref.16 Ref.17 Ref.23

Subcellular location: Cell membrane; Single-pass type I membrane protein Ref.15 Ref.16. Processed macrophage colony-stimulating factor 1: Secreted › extracellular space Ref.15 Ref.16.

Post-translational modification: N- and O-glycosylated. Glycosylation and proteolytic cleavage yield different soluble forms. One high molecular weight soluble form is a proteoglycan containing chondroitin sulfate. O-glycosylated with core 1 or possibly core 8 glycans. Isoform 1 is N- and O-glycosylated. Isoform 3 is only N-glycosylated. Ref.15 Ref.16 Ref.24 Ref.25

Involvement in disease: Aberrant expression of CSF1 or CSF1R can promote cancer cell proliferation, invasion and formation of metastases. Overexpression of CSF1 or CSF1R is observed in a significant percentage of breast, ovarian, prostate, and endometrial cancers. Ref.19 Ref.21Aberrant expression of CSF1 or CSF1R may play a role in inflammatory diseases, such as rheumatoid arthritis, glomerulonephritis, atherosclerosis, and allograft rejection. Ref.19 Ref.21

Research Articles on M-CSF

1. Nucleolin mediates microRNA-directed CSF-1 mRNA deadenylation but increases translation of CSF-1 mRNA.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-M-CSF antibody

Reference Product	PubMed Publications
CSF1R antibody	>74 publications with M-CSF and CSF1R
JUN antibody	>20 publications with M-CSF and JUN
GAPDH antibody	>3 publications with M-CSF and GAPDH
MYC antibody	>3 publications with M-CSF and MYC
ITGB3 antibody	>1 publications with M-CSF and ITGB3

Pathways associated with anti-M-CSF antibody

Products by Pathway	Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokines And Inflammatory Response Pathway antibodies	Cytokines And Inflammatory Response Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
Integrins In Angiogenesis Pathway antibodies	Integrins In Angiogenesis Pathway Diagram
Osteoclast Differentiation Pathway antibodies	Osteoclast Differentiation Pathway Diagram
Osteoclast Differentiation Pathway antibodies	Osteoclast Differentiation Pathway Diagram
PI3K-Akt Signaling Pathway antibodies	PI3K-Akt Signaling Pathway Diagram
PI3K-Akt Signaling Pathway antibodies	PI3K-Akt Signaling Pathway Diagram

Diseases associated with anti-M-CSF antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>63 publications with M-CSF and Neoplasms
Inflammation Antibodies	>27 publications with M-CSF and Inflammation
Carcinoma Antibodies	>21 publications with M-CSF and Carcinoma
Necrosis Antibodies	>15 publications with M-CSF and Necrosis
Breast Neoplasms Antibodies	>14 publications with M-CSF and Breast Neoplasms
Lung Neoplasms Antibodies	>9 publications with M-CSF and Lung Neoplasms
Neoplasms, Experimental Antibodies	>7 publications with M-CSF and Neoplasms, Experimental
Liver Diseases Antibodies	>7 publications with M-CSF and Liver Diseases
Osteitis Deformans Antibodies	>6 publications with M-CSF and Osteitis Deformans
Prostatic Neoplasms Antibodies	>5 publications with M-CSF and Prostatic Neoplasms

Organs/Tissues associated with anti-M-CSF antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>57 publications with M-CSF and Blood
Bone Antibodies	>53 publications with M-CSF and Bone
Lung Antibodies	>21 publications with M-CSF and Lung
Liver Antibodies	>15 publications with M-CSF and Liver
Brain Antibodies	>13 publications with M-CSF and Brain
Connective Tissue Antibodies	>10 publications with M-CSF and Connective Tissue
Kidney Antibodies	>8 publications with M-CSF and Kidney
Intestine Antibodies	>7 publications with M-CSF and Intestine
Lymph Node Antibodies	>7 publications with M-CSF and Lymph Node
Nerve Antibodies	>6 publications with M-CSF and Nerve