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TGF-alpha Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS691266

Transforming Growth Factor-&alpha (TGF-alpha was originally isolated from the conditioned media of oncogenically-transformed cells as an EGF-like bioactivity. TGF-alpha is a member of the EGF family of cytokines that are synthesized as transmembrane… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
TGF-alpha, Polyclonal Antibody
Full Product Name
Rabbit Anti-Human TGF-alpha
Product Gene Name
anti-TGF-alpha antibody
Product Synonym Names
Antigen-Affinity Purified Polyclonal Antibodies Anti-human TGF-alpha
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Lyophilized
Purity/Purification
Affinity chromatography
Molecular Weight
17,006 Da
Chromosome Location
Chromosome: 2; NC_000002.12 (70447280..70554015, complement). Location: 2p13
OMIM
190170
3D Structure
ModBase 3D Structure for P01135
Antibody Generation
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant human TGF alpha (human Transforming Growth Factor-alpha). Anti-human TGF alpha specific antibody was purified by affinity chromatography employing immobilized human TGF alpha matrix.
Buffer
PBS, pH 7.2
Neutralization
To yield one-half maximal inhibition [ND50] of the biological activity of human TGF-alpha (2 ng/ml), a concentration of 0.1 - 0.15 g/ml of this antibody is required.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-TGF-alpha antibody
ELISA (EIA), Western Blot (WB), Neutr
Application Notes for anti-TGF-alpha antibody
Western Blot: To detect human TGF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 g/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human TGF-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Other Notes

Small volumes of anti-TGF-alpha antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TGF-alpha purchase

MBS691266 is an antibody targeting TGF-alpha. Also known as Rabbit Anti-Human TGF-alpha. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TGF-alpha. It may not necessarily be applicable to this product.

NCBI GI #
153791414
NCBI GeneID
7039
NCBI Accession #
NP_001093161.1
UniProt Primary Accession #
P01135
UniProt Secondary Accession #
Q15577; Q53SK7; Q9BS56; Q9UEI3; Q9UKM1; Q9UKM2; Q9UKM3; A8K286
UniProt Related Accession #
P01135
GenBank Nucleotide #
NM_001099691.2
NCBI Official Full Name
protransforming growth factor alpha isoform 2 preproprotein
NCBI Official Synonym Full Names
transforming growth factor, alpha
NCBI Official Symbol
TGFA
NCBI Official Synonym Symbols
TFGA
UniProt Protein Name
Protransforming growth factor alpha
UniProt Synonym Protein Names
EGF-like TGF; ETGF; TGF type 1
Protein Family
Transforming growth factor
UniProt Gene Name
TGFA
UniProt Synonym Gene Names
TGF-alpha; ETGF
UniProt Entry Name
TGFA_HUMAN
NCBI Protein Information
protransforming growth factor alpha; TGF-alpha

NCBI Summary for TGF-alpha

This gene encodes a growth factor that is a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers, and it may also be involved in some cases of cleft lip/palate. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
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UniProt Comments for TGF-alpha

Function: TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.

Subunit structure: Interacts with the PDZ domains of MAGI3, SDCBP and SNTA1. The interaction with SDCBP, is required for the targeting to the cell surface. In the endoplasmic reticulum, in its immature form (i.e. with a prosegment and lacking full N-glycosylation), interacts with CNIH. In the Golgi apparatus, may form a complex with CNIH and GORASP2. Interacts (via cytoplasmic C-terminal domain) with NKD2. Ref.16 Ref.17 Ref.18

Subcellular location: Transforming growth factor alpha: Secreted › extracellular space. Protransforming growth factor alpha: Cell membrane; Single-pass type I membrane protein.

Tissue specificity: Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines. Ref.6

Sequence similarities: Contains 1 EGF-like domain.
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Research Articles on TGF-alpha

1. these data demonstrate that TACE-dependent TGFalpha shedding is a key process driving EGFR activation and subsequent proliferation and invasion in TNBC cell lines.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TGF-alpha antibody

 Reference Product  PubMed Publications
 EGFR antibody  >36 publications with TGF-alpha and EGFR
 TNF antibody  >7 publications with TGF-alpha and TNF
 ERBB2 antibody  >7 publications with TGF-alpha and ERBB2
 IL6 antibody  >5 publications with TGF-alpha and IL6
 ERBB4 antibody  >3 publications with TGF-alpha and ERBB4
 TP53 antibody  >3 publications with TGF-alpha and TP53
 ERBB3 antibody  >2 publications with TGF-alpha and ERBB3

Pathways associated with anti-TGF-alpha antibody

 Products by Pathway  Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 ErbB Receptor Signaling Network Pathway antibodies  ErbB Receptor Signaling Network Pathway Diagram
 ErbB Signaling Pathway antibodies  ErbB Signaling Pathway Diagram
 ErbB Signaling Pathway antibodies  ErbB Signaling Pathway Diagram
 ErbB Signaling Pathway antibodies  ErbB Signaling Pathway Diagram
 FOXM1 Transcription Factor Network Pathway antibodies  FOXM1 Transcription Factor Network Pathway Diagram
 Glioma Pathway antibodies  Glioma Pathway Diagram
 Glioma Pathway antibodies  Glioma Pathway Diagram
 Non-small Cell Lung Cancer Pathway antibodies  Non-small Cell Lung Cancer Pathway Diagram
 Non-small Cell Lung Cancer Pathway antibodies  Non-small Cell Lung Cancer Pathway Diagram

Diseases associated with anti-TGF-alpha antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >63 publications with TGF-alpha and Neoplasms
 Genetic Predisposition to Disease Antibodies  >17 publications with TGF-alpha and Genetic Predisposition to Disease
 Fatty Liver Antibodies  >16 publications with TGF-alpha and Fatty Liver
 Adenocarcinoma Antibodies  >15 publications with TGF-alpha and Adenocarcinoma
 Liver Diseases Antibodies  >10 publications with TGF-alpha and Liver Diseases
 Liver Neoplasms Antibodies  >10 publications with TGF-alpha and Liver Neoplasms
 Head and Neck Neoplasms Antibodies  >9 publications with TGF-alpha and Head and Neck Neoplasms
 Breast Neoplasms Antibodies  >9 publications with TGF-alpha and Breast Neoplasms
 Inflammation Antibodies  >7 publications with TGF-alpha and Inflammation
 Neoplasms, Experimental Antibodies  >7 publications with TGF-alpha and Neoplasms, Experimental

Organs/Tissues associated with anti-TGF-alpha antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >4 publications with TGF-alpha and Brain
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