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Rabbit Anti Human IgG Fab Secondary Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS571515

The antiserum is reacting with the Fab subunit of intact IgG, IgA and IgM and other Ig classes of both light chain types, with their Fab or F(ab’)2 subunits and with free light chains of kappa and lambda type as tested in immunoelectrophoresis and double… More details

$315 / 1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Rabbit anti Human IgG Fab, Secondary Antibody
Full Product Name
Rabbit anti Human IgG Fab
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
Form/Format
Delipidated, heat inactivated, lyophilized, stable whole serum. No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled rabbit serum. No foreign proteins added.
Specificity
Precipitating polyclonal Rabbit antiSerum to Human Fab of IgG.
Preparation and Storage
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Source
Purified Fab of normal human IgG. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Product
Delipidated, heat inactivated, lyophilized, stable whole serum. No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled rabbit serum. No foreign proteins added. It is reconstituted by adding 1 ml sterile distilled water.

Product Categories/Family for Rabbit anti Human IgG Fab secondary antibody

Antisera
Applications Tested/Suitable for Rabbit anti Human IgG Fab secondary antibody
Immunoelectrophoresis
Application Notes for Rabbit anti Human IgG Fab secondary antibody
Precipitation assays. In immunoelectrophoresis use 2 ?l or equivalent against 120 ul antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ul antiserum in a 3 mm diameter centre well and 2 ul serum samples (neat and diluted) in 2 mm diameter peripheral wells.

Other Notes

Small volumes of Rabbit anti Human IgG Fab secondary antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Rabbit anti Human IgG Fab purchase

MBS571515 is a secondary antibody targeting Rabbit anti Human IgG Fab. Reactive in Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.. Host: Rabbit.
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