Rabbit Anti-MCP-1/MCAF Polyclonal Antibody – Cat# MBS691304

MCP-1/MCAF Antibody – Rabbit Polyclonal

Name: MCP-1/MCAF Antibody; Polyclonal; Rabbit; Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS691304
Price: 605 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS691304

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat MCP-I/MCAF (Macrophage/Monocyte chemotactic protein-I). Anti-Rat MCP-I/MCAF specific antibody was purified by affinity chromatography employing immobilized Rat MCP-I/MCAF… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

MCP-1/MCAF, Polyclonal Antibody

Full Product Name

Rabbit Anti-Rat MCP-1/MCAF

Product Gene Name

anti-MCP-1/MCAF antibody

Product Synonym Names

Antigen-Affinity Purified Polyclonal Antibodies Anti-rat MCP-1/MCAF

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Rat

Form/Format

Lyophilized

Purity/Purification

Affinity chromatography

Preparation and Storage

The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20 degree C. When reconstituted in sterile water to a concentration of 20.2 mg/ml the antibody is stable for at least six weeks at 2-4 degree C. Avoid repeated freeze-thaw cycles.

Molecular Weight

16,460 Da

Chromosome Location

Chromosome: 10; NC_005109.3 (69047091..69048889). Location: 10q26

3D Structure

ModBase 3D Structure for P14844

Antigen

Recombinant rat MCP-1/MCAF

Usage

Anti rat MCP-l/MCAF is offered for research use. Not for drug use. Not for human use.

Buffer

PBS; pH 7.2

Neutralization

To yield one-half maximal inhibition [ND50] of the biological activity of Rat MCP-1/MCAF (100 ng/ml), a concentration of 2.0 - 4.0 ug/ml of this antibody is required.

Note

Always centrifuge vials before opening.

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-MCP-1/MCAF antibody

ELISA (EIA), Western Blot (WB), Neutr

Application Notes for anti-MCP-1/MCAF antibody

Neutralization: To yield one-half maximal inhibition [NDsoJ of the biological activity of Rat MCP-I (MCAF)(l 00 ng/ml), a concentration of 4.0-6.0 Ilg/ml of this antibody is required.

Sandwich ELISA: To detect Rat MCP-I/MCAF by sandwich ELISA (using 100 Ill/well antibody solution) a concentration of at least 0.5 - 2 Ilg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant Rat MCP-I/MCAF.

Western Blot: To detect Rat MCP-l/MCAF by Western Blot analysis thIs antibody can be used at a concentration of 0.1 - 0.2 Ilg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat MCP-I/MCAF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Other Notes

Small volumes of anti-MCP-1/MCAF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MCP-1/MCAF purchase

MBS691304 is an antibody targeting MCP-1/MCAF. Also known as Rabbit Anti-Rat MCP-1/MCAF. Reactive in Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MCP-1/MCAF. It may not necessarily be applicable to this product.

NCBI GI #

13928714

NCBI GeneID

24770

NCBI Accession #

NP_113718.1

UniProt Primary Accession #

P14844

UniProt Secondary Accession #

Q549R5

UniProt Related Accession #

P14844

GenBank Nucleotide #

NM_031530.1

NCBI Official Full Name

C-C motif chemokine 2

NCBI Official Synonym Full Names

chemokine (C-C motif) ligand 2

NCBI Official Symbol

Ccl2

NCBI Official Synonym Symbols

MCP-1; Scya2; Sigje

UniProt Protein Name

C-C motif chemokine 2

UniProt Synonym Protein Names

Immediate-early serum-responsive protein JE; Monocyte chemoattractant protein 1; Monocyte chemotactic protein 1; MCP-1; Small-inducible cytokine A2

UniProt Gene Name

Ccl2

UniProt Synonym Gene Names

Je; Mcp1; Scya2; MCP-1

UniProt Entry Name

CCL2_RAT

NCBI Protein Information

C-C motif chemokine 2; small inducible gene JE; small inducible cytokine A2; small-inducible cytokine A2; monocyte chemotactic protein 1; monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; immediate-early serum-responsive JE protein; immediate-early serum-responsive protein JE

NCBI Summary for MCP-1/MCAF

a monocyte chemoattractant protein [RGD, Feb 2006]

UniProt Comments for MCP-1/MCAF

Function: Chemotactic factor that attracts monocytes, but not neutrophils.

Subunit structure: Monomer or homodimer; in equilibrium

By similarity.

Subcellular location: Secreted.

Sequence similarities: Belongs to the intercrine beta (chemokine CC) family.

Research Articles on MCP-1/MCAF

1. Suggest Adrb1/2 system in spinal astrocytes could play a key role in spinal functioning through the regulation of JNK1 activity and CCL2 production.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MCP-1/MCAF antibody

Reference Product	PubMed Publications
Tnf antibody	>1784 publications with MCP-1/MCAF and Tnf
Ccr2 antibody	>1148 publications with MCP-1/MCAF and Ccr2
Ccl3 antibody	>826 publications with MCP-1/MCAF and Ccl3
Icam1 antibody	>635 publications with MCP-1/MCAF and Icam1
Ccr5 antibody	>313 publications with MCP-1/MCAF and Ccr5
Ccl7 antibody	>233 publications with MCP-1/MCAF and Ccl7
Il6 antibody	>217 publications with MCP-1/MCAF and Il6
Ccr1 antibody	>175 publications with MCP-1/MCAF and Ccr1
Cx3cr1 antibody	>107 publications with MCP-1/MCAF and Cx3cr1
Il1b antibody	>43 publications with MCP-1/MCAF and Il1b

Pathways associated with anti-MCP-1/MCAF antibody

Products by Pathway	Pathway Diagram
Activation Of Genes By ATF4 Pathway antibodies	Activation Of Genes By ATF4 Pathway Diagram
Chagas Disease (American Trypanosomiasis) Pathway antibodies	Chagas Disease (American Trypanosomiasis) Pathway Diagram
Chagas Disease (American Trypanosomiasis) Pathway antibodies	Chagas Disease (American Trypanosomiasis) Pathway Diagram
Chemokine Receptors Bind Chemokines Pathway antibodies	Chemokine Receptors Bind Chemokines Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram

Diseases associated with anti-MCP-1/MCAF antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>3932 publications with MCP-1/MCAF and Inflammation
Necrosis Antibodies	>2358 publications with MCP-1/MCAF and Necrosis
Cardiovascular Diseases Antibodies	>1719 publications with MCP-1/MCAF and Cardiovascular Diseases
Atherosclerosis Antibodies	>969 publications with MCP-1/MCAF and Atherosclerosis
Kidney Diseases Antibodies	>788 publications with MCP-1/MCAF and Kidney Diseases
Myocardial Ischemia Antibodies	>373 publications with MCP-1/MCAF and Myocardial Ischemia
Liver Diseases Antibodies	>306 publications with MCP-1/MCAF and Liver Diseases
Hypersensitivity Antibodies	>301 publications with MCP-1/MCAF and Hypersensitivity
Coronary Artery Disease Antibodies	>297 publications with MCP-1/MCAF and Coronary Artery Disease
Glomerulonephritis Antibodies	>274 publications with MCP-1/MCAF and Glomerulonephritis

Organs/Tissues associated with anti-MCP-1/MCAF antibody

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>1010 publications with MCP-1/MCAF and Lung
Heart Antibodies	>603 publications with MCP-1/MCAF and Heart
Liver Antibodies	>592 publications with MCP-1/MCAF and Liver
Connective Tissue Antibodies	>380 publications with MCP-1/MCAF and Connective Tissue
Prostate Antibodies	>77 publications with MCP-1/MCAF and Prostate
Dorsal Root Ganglion Antibodies	>49 publications with MCP-1/MCAF and Dorsal Root Ganglion