PlGF Rat Active Protein

Placenta growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family of growth factors. PlGF and VEGF share primary structural as well as limited amino acid sequence homology with the A and B chains of PDGF. All eight cysteine residues involved in intra and interchain disulfides are conserved among these growth factors. As a result of alternative splicing, three PlGF RNAs encoding monomeric human PlGF-1, PlGF-2 and PlGF-3 isoform precursors containing 149, 179 and 219 amino acid residues, respectively, have been described. In normal mouse and rat tissues, only one PlGF mRNA encoding the equivalent of human PlGF-2 has been identified. Rat PlGF shares about 60% amino acid identity with human PlGF-2. The gene for PlGF has been mapped to rat chromosome 6. PlGF binds with high affinity to Flt1, but not to Flk1/KDR.
However, little information regarding the expression pattern and cellular localization of PlGF mRNA in rat placenta during pregnancy is known. RT-PCR analysis shows that the expression level of PlGF mRNA increased as gestation advanced. Using in situ hybridization histochemistry, positive cells of PlGF mRNA were detected in chorionic villi, in the trophoblast and stroma cells surrounding the blood vessels within chorionic villi on day 13 and 15. The expression pattern of PlGF mRNA in rat placenta during pregnancy demonstrates that PlGF plays a functional role for placental growth and fetal development during mid-late pregnancy.
The full ORF of rat PlGF (Met1-Leu158) was cloned from total RNA of rat sinusoidal endothelial cells using standard protocols. The native protein expressed in insect cells starts with Ala24.

1. Osol G et al, Am J Physiol Heart Circ Physiol 294, 2008 2. Choi WS et al, J Vet Sci 6(3), 2005 3. Koh PO et al, J Vet Med Sci 69(9), 2007 4. Torry RJ et al, J Heart Lung Transplant 28(2), 2009 5. Sands M et al, Respir Res 12, 2011

Small volumes of PlGF active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS691573 is an active protein targeting PlGF. Also known as Rat PlGF. Reactive in Rat. Host: Insect Cells.