Human TPO Active Protein – Cat# MBS691688

TPO Human Active Protein

Name: TPO Active Protein; Human; MyBioSource
Brand: MyBioSource
SKU: MBS691688
Price: 1260 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS691688

TPO is a lineage specific growth factor, produced in the liver, kidney and skeletal muscle. It stimulates the proliferation and maturation of megakaryocytes, and promotes increased circulating levels of platelets in vivo. TPO signals through the c-mpl… More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

TPO, Active Protein

Full Product Name

Human TPO

Product Gene Name

TPO active protein

Product Synonym Names

Recombinant Human TPO

Sequence

SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDF SLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL G QLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRG KVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNEL

Host

E Coli

Species Reactivity

Human

Form/Format

Lyophilized

Purity/Purification

> 98% by RP-HPLC, Anion-exchange FPLC, Silverstain

Molecular Weight

18.6 kDa

Chromosome Location

Chromosome: 3; NC_000003.12 (184371985..184379625, complement). Location: 3q27

OMIM

187950

3D Structure

ModBase 3D Structure for P40225

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Endotoxin Level

< 0.1 ng per ug (IEU/ug) of rh TPO

Biological Activity

The ED50 as determined by the dose-dependant stimulation of MO7e cells is < 1 ng/ml (1xE6 units/mg).

Product Categories/Family for TPO active protein

Cytokines & Growth Factors

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Other Notes

Small volumes of TPO active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TPO purchase

MBS691688 is an active protein targeting TPO. Also known as Human TPO. Reactive in Human. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for TPO. It may not necessarily be applicable to this product.

NCBI GI #

4507493

NCBI GeneID

7066

NCBI Accession #

NP_000451.1

UniProt Primary Accession #

P40225

UniProt Secondary Accession #

Q13020; Q15790; Q15791; Q15792; A1L3Y0; B7ZLR8; B9EGA8

UniProt Related Accession #

P40225

GenBank Nucleotide #

NM_000460.2

NCBI Official Full Name

thrombopoietin isoform 1

NCBI Official Synonym Full Names

thrombopoietin

NCBI Official Symbol

THPO

NCBI Official Synonym Symbols

ML; TPO; MGDF; MKCSF; MPLLG; THCYT1

UniProt Protein Name

Thrombopoietin

UniProt Synonym Protein Names

C-mpl ligand; ML; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGDF; Myeloproliferative leukemia virus oncogene ligand

Protein Family

Thyroid peroxidase

UniProt Gene Name

THPO

UniProt Synonym Gene Names

MGDF; ML; MGDF

UniProt Entry Name

TPO_HUMAN

NCBI Protein Information

thrombopoietin; MPL ligand; c-mpl ligand; prepro-thrombopoietin; megakaryocyte stimulating factor; megakaryocyte colony-stimulating factor; megakaryocyte growth and development factor; myeloproliferative leukemia virus oncogene ligand

NCBI Summary for TPO

Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent. [provided by RefSeq, Feb 2014]

UniProt Comments for TPO

Function: Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.

Subcellular location: Secreted.

Domain: Two-domain structure with an erythropoietin-like N-terminal and a Ser/Pro/Thr-rich C-terminal.

Involvement in disease: Thrombocythemia 1 (THCYT1) [MIM:187950]: A myeloproliferative disorder characterized by excessive platelet production, resulting in increased numbers of circulating platelets. It can be associated with spontaneous hemorrhages and thrombotic episodes.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.10

Sequence similarities: Belongs to the EPO/TPO family.

Research Articles on TPO

1. An Arg->Cys substitution at residue 38 or residue 17 excluding the 21-AA signal peptide of the receptor binding domain was found in a family with aplastic anemia. Adding a 5th cysteine may disrupt normal disulfide bonding & receptor binding.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with TPO active protein

Reference Product	PubMed Publications
MPL active protein	>22 publications with TPO and MPL
KITLG active protein	>7 publications with TPO and KITLG
SELP active protein	>1 publications with TPO and SELP

Pathways associated with TPO active protein

Products by Pathway	Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram
Platelet Aggregation (Plug Formation) Pathway antibodies	Platelet Aggregation (Plug Formation) Pathway Diagram
Platelet Activation, Signaling And Aggregation Pathway antibodies	Platelet Activation, Signaling And Aggregation Pathway Diagram

Diseases associated with TPO active protein

Disease Name	Pubmed Publications
Hematologic Diseases Antibodies	>16 publications with TPO and Hematologic Diseases
Neoplasms Antibodies	>10 publications with TPO and Neoplasms
Thrombocytosis Antibodies	>8 publications with TPO and Thrombocytosis
Lung Neoplasms Antibodies	>3 publications with TPO and Lung Neoplasms
Carcinoma Antibodies	>3 publications with TPO and Carcinoma
Carcinoma, Squamous Cell Antibodies	>2 publications with TPO and Carcinoma, Squamous Cell
Thrombocythemia, Essential Antibodies	>2 publications with TPO and Thrombocythemia, Essential
Heart Failure Antibodies	>1 publications with TPO and Heart Failure
Hypertension Antibodies	>1 publications with TPO and Hypertension
Precancerous Conditions Antibodies	>1 publications with TPO and Precancerous Conditions