Human CD14 Active Protein – Cat# MBS691707

C1 Inhibitor (CD14) Human Active Protein

Name: CD14 Active Protein; C1 Inhibitor; Human; MyBioSource
Brand: MyBioSource
SKU: MBS691707
Price: 305 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS691707

CD14 is a cell surface anchored glycoprotein that is expressed predominantly by monocytes and tissue macrophages. CD14 associates with MD-2 (LY-96) and TLR4 to form a receptor complex, which signals specifically in response to bacterial lipopolysaccharide… More details

$305 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

C1 Inhibitor (CD14), Active Protein

Full Product Name

Human CD14, soluble

Product Gene Name

CD14 active protein

Product Synonym Names

Plasma protease C1; C1inh; C1 Esterase Protein; C1-inhibiting factor; Serpin G1; soluble CD14

Sequence

TTPEPCELDD EDFRCVCNFS EPQPDWSEAF QCVSAVEVEI HAGGLN LEPF LKRVDADADP RQYADTVKAL RVRRLTVGAA QVPAQLLVGA L RVLAYSRLK ELTLEDLKIT GTMPPLPLEA TGLALSSLRL RNVSWAT GRS WLAELQQWLK PGLKVLSIAQ AHSPAFSCEQ VRAFPALTSL DL SDNPGLGE RGLMAALCPH KFPAIQNLAL RNTGMETPTG VCAALAAA GV QPHSLDLSHN SLRATVNPSA PRCMWSSALN SLNLSFAGLE QVP KGLPAKL RVLDLSCNRL NRAPQPDELP EVDNLTLDGN PFLVPGTAL P HEGSMNSGVV P

Host

HEK 293 cells

Species Reactivity

Human

Form/Format

Lyophilized from 10mM Sodium Phosphate, pH 7.5

Purity/Purification

> 95% by SDS-PAGE gel and HPLC analysis.

Preparation and Storage

Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20 degree C to -80 degree C.

Stability: Lyophilized CD14 should be stored desiccated below -20 degree C. Upon reconstitution CDNF should be stored at 4 degree C between 2-7 days and for future use below -20 degree C. For long term storage it is recommended to add a carrier protein (0.1% HAS or BSA). Avoid repeated freeze-thaw cycles.

Molecular Weight

35.6 kDa

Chromosome Location

Chromosome: 5; NC_000005.10 (140631728..140633701, complement). Location: 5q31.1

OMIM

158120

3D Structure

ModBase 3D Structure for P08571

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Endotoxin level

<0.1ng per ug of human CD14

Biological Activity

Determined by the dose dependent activation of NF-kappaB in a RAW264 cell line based reporter system, using a sCD14 concentration range of 20 ng/ ul to 200 ng/ ul. The NF-kappaB activation is enhanced when the assay is done in the presence of 0.25 ng/ ul to 1.0 ng/ ul bacterial LPS.

Product Categories/Family for CD14 active protein

Soluble receptors

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Other Notes

Small volumes of CD14 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD14 purchase

MBS691707 is an active protein targeting CD14. Also known as Human CD14, soluble. Reactive in Human. Host: HEK 293 cells.

Additional Details

NCBI/Uniprot data below describe general gene information for CD14. It may not necessarily be applicable to this product.

NCBI GI #

4557417

NCBI GeneID

929

NCBI Accession #

NP_000582.1

UniProt Primary Accession #

P08571

UniProt Secondary Accession #

Q53XT5; Q96FR6; Q96L99; Q9UNS3

UniProt Related Accession #

P08571

GenBank Nucleotide #

NM_000591.3

NCBI Official Full Name

monocyte differentiation antigen CD14

NCBI Official Synonym Full Names

CD14 molecule

NCBI Official Symbol

CD14

UniProt Protein Name

Monocyte differentiation antigen CD14

UniProt Synonym Protein Names

Myeloid cell-specific leucine-rich glycoprotein

Protein Family

Monocyte differentiation antigen

UniProt Gene Name

CD14

UniProt Entry Name

CD14_HUMAN

NCBI Protein Information

monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein

NCBI Summary for CD14

The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Mar 2010]

UniProt Comments for CD14

Function: In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. Ref.15

Subunit structure: Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, MD-2 and TLR4.

Subcellular location: Cell membrane; Lipid-anchor › GPI-anchor.

Tissue specificity: Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.

Post-translational modification: N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.

Sequence similarities: Contains 11 LRR (leucine-rich) repeats.

Research Articles on CD14

1. these results suggest that membrane CD14 ligation-mediated TLR4 internalization is a novel mechanism for effective down-regulation of surface expression of TLR4 and subsequent reduction of LPS response of human monocytes.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CD14 active protein

Reference Product	PubMed Publications
TLR4 active protein	>1275 publications with CD14 and TLR4
TLR2 active protein	>681 publications with CD14 and TLR2
LBP active protein	>413 publications with CD14 and LBP
ITGB2 active protein	>309 publications with CD14 and ITGB2
LY96 active protein	>140 publications with CD14 and LY96
TLR1 active protein	>91 publications with CD14 and TLR1
TLR6 active protein	>58 publications with CD14 and TLR6
LY86 active protein	>9 publications with CD14 and LY86

Pathways associated with CD14 active protein

Products by Pathway	Pathway Diagram
Activated TLR4 Signalling Pathway antibodies	Activated TLR4 Signalling Pathway Diagram
Activation Of IRF3/IRF7 Mediated By TBK1/IKK Epsilon Pathway antibodies	Activation Of IRF3/IRF7 Mediated By TBK1/IKK Epsilon Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
IKK Complex Recruitment Mediated By RIP1 Pathway antibodies	IKK Complex Recruitment Mediated By RIP1 Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Innate Immune System Pathway antibodies	Innate Immune System Pathway Diagram
Legionellosis Pathway antibodies	Legionellosis Pathway Diagram

Diseases associated with CD14 active protein

Disease Name	Pubmed Publications
Necrosis Antibodies	>2196 publications with CD14 and Necrosis
Inflammation Antibodies	>2066 publications with CD14 and Inflammation
Neoplasms Antibodies	>1462 publications with CD14 and Neoplasms
Cardiovascular Diseases Antibodies	>582 publications with CD14 and Cardiovascular Diseases
Lung Diseases Antibodies	>496 publications with CD14 and Lung Diseases
Nervous System Diseases Antibodies	>333 publications with CD14 and Nervous System Diseases
Liver Diseases Antibodies	>328 publications with CD14 and Liver Diseases
Atherosclerosis Antibodies	>281 publications with CD14 and Atherosclerosis
Heart Diseases Antibodies	>245 publications with CD14 and Heart Diseases
Asthma Antibodies	>244 publications with CD14 and Asthma

Organs/Tissues associated with CD14 active protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>7061 publications with CD14 and Blood
Bone Antibodies	>1243 publications with CD14 and Bone
Vascular Antibodies	>769 publications with CD14 and Vascular
Liver Antibodies	>573 publications with CD14 and Liver
Lung Antibodies	>552 publications with CD14 and Lung
Kidney Antibodies	>309 publications with CD14 and Kidney
Brain Antibodies	>279 publications with CD14 and Brain
Muscle Antibodies	>261 publications with CD14 and Muscle
Heart Antibodies	>257 publications with CD14 and Heart
Embryonic Tissue Antibodies	>216 publications with CD14 and Embryonic Tissue