Human IL-12 Active Protein – Cat# MBS691778

IL-12 Human Active Protein

Name: IL-12 Active Protein; Human; MyBioSource
Brand: MyBioSource
SKU: MBS691778
Price: 305 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS691778

IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue type mast cells. Among its biological activities… More details

$305 / 0.002 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

IL-12, Active Protein

Full Product Name

Human IL-12

Product Gene Name

IL-12 active protein

Product Synonym Names

Recombinant Human IL-12

Sequence

p35 Subunit: RNLPVA TPDPGMFPCL HHSQNLLRAV SNMLQKAR QT LEFYPCTSEE IDHEDITKDK TSTVEACLPL ELTKNESCLN SRE TSFITNG SCLASRKTSF MMALCLSSIY EDLKMYQVEF KTMNAKLLM D PKRQIFLDQN MLAVIDELMQ ALNFNSETVP QKSSLEEPDF YKTK IKLCIL LHAFRIRAVT IDRVMSYLNA S p40 Subunit: IWELKK DVYVVELDWY PDAPGEMVVL TCDTPEEDGI TWTLDQSSEV LGSGK TLTIQ VKEFGDAGQY TCHKGGEVLS HSLLLLHKKE DGIWSTDILK DQKEPKNKTF LRCEAKNYSG RFTCWWLTTI STDLTFSVKS SRGSSD PQGV TCGAATLSAE RVRGDNKEYE YSVECQEDSA CPAAEESLPI E VMVDAVHKL KYENYTSSFF IRDIIKPDPP KNLQLKPLKN SRQVEVS WEY PDTWSTPHSY FSLTFCVQVQ GKSKREKKDR VFTDKTSATV IC RKNASISV RAQDRYYSSS WSEWASVPCS

Host

CHO Cells

Species Reactivity

Human

Form/Format

Lyophilized

Purity/Purification

> 98% by SDS-PAGE and HPLC analyses

Molecular Weight

75 kDa

Chromosome Location

Chromosome: 3; NC_000003.12 (159988836..159996019). Location: 3q25.33

OMIM

161560

3D Structure

ModBase 3D Structure for P29459

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Endotoxin Level

< 0.1 ng per g of IL-12

Biological Activity

The ED50 was determined by the induction of IFN-gamma from NK cells co-stimulated with IL-18 is <= 1.0 ng/ml, corresponding to a specific activity of >= 1 x 106 units/mg.

Product Categories/Family for IL-12 active protein

Cytokines & Growth Factors

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Other Notes

Small volumes of IL-12 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL-12 purchase

MBS691778 is an active protein targeting IL-12. Also known as Human IL-12. Reactive in Human. Host: CHO Cells.

Additional Details

NCBI/Uniprot data below describe general gene information for IL-12. It may not necessarily be applicable to this product.

NCBI GI #

24430219

NCBI GeneID

3592

NCBI Accession #

NP_000873.2

UniProt Primary Accession #

P29459

UniProt Secondary Accession #

Q96QZ1

UniProt Related Accession #

P29459

GenBank Nucleotide #

NM_000882.3

NCBI Official Full Name

interleukin-12 subunit alpha

NCBI Official Synonym Full Names

interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)

NCBI Official Symbol

IL12A

NCBI Official Synonym Symbols

P35; CLMF; NFSK; NKSF1; IL-12A

UniProt Protein Name

Interleukin-12 subunit alpha

UniProt Synonym Protein Names

Cytotoxic lymphocyte maturation factor 35 kDa subunit; CLMF p35; IL-12 subunit p35; NK cell stimulatory factor chain 1

UniProt Gene Name

IL12A

UniProt Synonym Gene Names

NKSF1; IL-12A; CLMF p35; NKSF1

UniProt Entry Name

IL12A_HUMAN

NCBI Protein Information

interleukin-12 subunit alpha; CLMF p35; IL35 subunit; IL-12, subunit p35; interleukin 12, p35; interleukin-12 alpha chain; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; cytotoxic lymphocyte maturation factor 1, p35; cytotoxic lymphocyte maturation factor 35 kDa subunit

NCBI Summary for IL-12

This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008]

UniProt Comments for IL-12

Function: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.

Subunit structure: Heterodimer with IL12B; disulfide-linked. The heterodimer is known as interleukin IL-12. Ref.8

Subcellular location: Secreted.

Induction: Down-regulated in response to enterovirus 71 (EV71) infection. Ref.7

Sequence similarities: Belongs to the IL-6 superfamily.

Sequence caution: The sequence AAA59937.1 differs from that shown. Reason: Erroneous initiation.

Research Articles on IL-12

1. IL-35 is an anti-inflammatory cytokine comprising the p35 subunit of IL-12 and the subunit Epstein-Barr virus (EBV) -induced gene 3(EBI3)--{REVIEW}

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with IL-12 active protein

Reference Product	PubMed Publications
IL12B active protein	>45 publications with IL-12 and IL12B
IL4 active protein	>17 publications with IL-12 and IL4
IFNG active protein	>14 publications with IL-12 and IFNG
IL12RB2 active protein	>11 publications with IL-12 and IL12RB2
IL1B active protein	>10 publications with IL-12 and IL1B
IL12RB1 active protein	>9 publications with IL-12 and IL12RB1
STAT1 active protein	>4 publications with IL-12 and STAT1

Pathways associated with IL-12 active protein

Products by Pathway	Pathway Diagram
African Trypanosomiasis Pathway antibodies	African Trypanosomiasis Pathway Diagram
African Trypanosomiasis Pathway antibodies	African Trypanosomiasis Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Chagas Disease (American Trypanosomiasis) Pathway antibodies	Chagas Disease (American Trypanosomiasis) Pathway Diagram
Chagas Disease (American Trypanosomiasis) Pathway antibodies	Chagas Disease (American Trypanosomiasis) Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram

Diseases associated with IL-12 active protein

Disease Name	Pubmed Publications
Neoplasms Antibodies	>34 publications with IL-12 and Neoplasms
Inflammation Antibodies	>18 publications with IL-12 and Inflammation
Necrosis Antibodies	>15 publications with IL-12 and Necrosis
Liver Diseases Antibodies	>9 publications with IL-12 and Liver Diseases
Disease Models, Animal Antibodies	>9 publications with IL-12 and Disease Models, Animal
Multiple Sclerosis Antibodies	>9 publications with IL-12 and Multiple Sclerosis
Cholestasis Antibodies	>7 publications with IL-12 and Cholestasis
Liver Cirrhosis, Biliary Antibodies	>7 publications with IL-12 and Liver Cirrhosis, Biliary
Brain Diseases Antibodies	>6 publications with IL-12 and Brain Diseases
Neoplasms, Experimental Antibodies	>5 publications with IL-12 and Neoplasms, Experimental

Organs/Tissues associated with IL-12 active protein

Organ/Tissue Name	Pubmed Publications
Spleen Antibodies	>4 publications with IL-12 and Spleen
Uterus Antibodies	>2 publications with IL-12 and Uterus
Brain Antibodies	>2 publications with IL-12 and Brain