• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

PAF-AH Human Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS691937

Platelet Activating Factor (PAF) is a biologically active phospholipid, which exerts primarily proinflammatory activities by specifically signaling through G-protein-coupled receptors on platelets, neutrophils, and monocytes. Platelet Activating Factor… More details

$305 / 0.005 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PAF-AH, Active Protein
Full Product Name
Human PAF-AH
Product Gene Name
Product Synonym Names
PAF-acetylhydrolase; PAF 2-acylhydrolase; LDL-PLA82); 2-acetyl-1-alkylglycerophosphacholine esterase
Sequence
FDWQYINPVA HMKSSAWVNK IQVLMAAASF GQTKIPRGNG PYSVGC TDLM FDHTNKGTFL RLYYPSQDND RLDTLWIPNK EYFWGLSKFL G THWLMGNIL RLLFGSMTTP ANWNSPLRPG EKYPLVVFSH GLGAFRT LYS AIGIDLASHG FIVAAVEHRD RSASATYYFK DQSAAEIGDK SW LYLRTLKQ EEETHIRNEQ VRQRAKECSQ ALSLILDIDH GKPVKNAL DL KFDMEQLKDS IDREKIAVIG HSFGGATVIQ TLSEDQRFRC GIA LDAWMFP LGDEVYSRIP QPLFFINSEY FQYPANIIKM KKCYSPDKE R KMITIRGSVH QNFADFTFAT GKIIGHMLKL KGDIDSNVAI DLSN KASLAF LQKHLGLHKD FDQWDCLIEG DDENLIPGTN INTTNQHIML QNSSGIEKYN
Host
HEK 293 Cells
Species Reactivity
Human
Form/Format
Lyophilized
Purity/Purification
> 95% by SDS-PAGE & HPLC analyses
Molecular Weight
25,569 Da
Chromosome Location
Chromosome: 11; NC_000011.10 (117144284..117178173). Location: 11q23
OMIM
602508
3D Structure
ModBase 3D Structure for P68402
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Biological Activity
Measured by its ability to cleave a PAF analog in a chromogenic substrate linked assay. At a PAF-AH concentration of 10.0 ug/ml, 50% cleavage was achieved at an incubation time of approximately 2 minutes.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.

Other Notes

Small volumes of PAF-AH active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PAF-AH purchase

MBS691937 is an active protein targeting PAF-AH. Also known as Human PAF-AH. Reactive in Human. Host: HEK 293 Cells.

Additional Details

NCBI/Uniprot data below describe general gene information for PAF-AH. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
platelet-activating factor acetylhydrolase IB subunit beta isoform b
NCBI Official Synonym Full Names
platelet-activating factor acetylhydrolase 1b, catalytic subunit 2 (30kDa)
NCBI Official Symbol
PAFAH1B2
NCBI Official Synonym Symbols
HEL-S-303
UniProt Protein Name
Platelet-activating factor acetylhydrolase IB subunit beta
UniProt Synonym Protein Names
PAF acetylhydrolase 30 kDa subunit; PAF-AH 30 kDa subunit; PAF-AH subunit beta
UniProt Gene Name
PAFAH1B2
UniProt Synonym Gene Names
PAFAHB; PAF-AH 30 kDa subunit
UniProt Entry Name
PA1B2_HUMAN
NCBI Protein Information
platelet-activating factor acetylhydrolase IB subunit beta; PAFAH subunit beta; PAF-AH subunit beta; PAF-AH 30 kDa subunit; PAF-AH1b alpha 2 subunit; PAF acetylhydrolase 30 kDa subunit; epididymis secretory protein Li 303; intracellular platelet-activating factor acetylhydrolase alpha 2 subunit; platelet-activating factor acetylhydrolase, isoform Ib, subunit 2 (30kDa)

NCBI Summary for PAF-AH

Platelet-activating factor acetylhydrolase (PAFAH) inactivates platelet-activating factor (PAF) into acetate and LYSO-PAF. This gene encodes the beta subunit of PAFAH, the other subunits are alpha and gamma. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jan 2014]
Show more

UniProt Comments for PAF-AH

Function: Inactivates PAF by removing the acetyl group at the sn-2 position. This is a catalytic subunit.

Catalytic activity: 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.

Subunit structure: Cytosolic PAF-AH IB is formed of three subunits of 45 kDa (alpha), 30 kDa (beta) and 29 kDa (gamma). The catalytic activity of the enzyme resides in the beta and gamma subunits, whereas the alpha subunit has regulatory activity. Trimer formation is not essential for the catalytic activity.

Subcellular location: Cytoplasm.

Tissue specificity: Ubiquitous. Ref.1

Sequence similarities: Belongs to the 'GDSL' lipolytic enzyme family. Platelet-activating factor acetylhydrolase IB beta/gamma subunits subfamily.
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000