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Arginine Deiminase (ADI) Mycoplasma Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS691980

Arginine Deiminase (ADI) is a microbial enzyme from Mycoplasma produced in E.coli. It has high affinity to L-arginine and hydrolyzes L-arginine to citrulline and ammonia. Low concentrations of ADI have been shown to inhibit proliferation in certain cultured… More details

$305 / 0.005 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Arginine Deiminase (ADI), Active Protein
Full Product Name
Mycoplasma Arginine Deiminase (ADI)
Product Gene Name
Product Synonym Names
ADI
Sequence
SVFDSKFKGI HVYSEIGELE SVLVHEPGRE IDYITPARLD ELLFSA ILES HDARKEHKQF VAELKANDIN VVELIDLVAE TYDLASQEAK D KLIEEFLED SEPVLSEEHK VVVRNFLKAK KTSRELVEIM MAGITKT DLG IEADHELIVD PMPNLYFTRD PFASVGNGVT IHYMRYKVRQ RE TLFSRFVF SNHPKLINTP WYYDPSLKLS IEGGDVFIYN NDTLVVGV SE RTDLQTVTLL AKNIVANKEC EFKRIVAINV PKWTNLMHLD TWL TMLDKDK FLYSPIANDV FKFWDYDLVN GGAEPQPVEN GLPLEGLLQ S IINKKPVLIP IAGEGASQME IERETHFDGT NYLAIRPGVV IGYS RNEKTN AALEAAGIKV LPFHGNQLSL GMGNARCMSM PLSRKDVKW
Host
E Coli
Form/Format
Lyophilized from 2mM Sodium Phosphate, pH7.5 + 0.2mM DTT
Purity/Purification
> 98% by SDS-PAGE & HPLC analysis
Preparation and Storage
The lyophilized protein is stable at room temperature for 1 month and at 4 degree C for 3 months. Reconstituted working aliquots are stable for 1 week at 2 degree C to 8 degree C and for 3 months at -20 degree C to -80 degree C.
Molecular Weight
46.3kDa
3D Structure
ModBase 3D Structure for P23793
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Endotoxin level
< 0.1 ng /ug of protein (<1EU/ug).
Biological Activity
Measured by its ability to induce apoptosis in Jurkat cells using a concentration of 100-150 ng/ml.
Protein RefSeq
CAA38080.1
mRNA RefSeq
AF182646.1
Reconstitution
Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20 degree C to -80 degree C.

Product Categories/Family for ADI active protein

ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.

Other Notes

Small volumes of ADI active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ADI purchase

MBS691980 is an active protein targeting ADI. Also known as Mycoplasma Arginine Deiminase (ADI). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for ADI. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
UniProt Primary Accession #
NCBI Official Full Name
Arginine deiminase
UniProt Protein Name
Arginine deiminase
UniProt Synonym Protein Names
Arginine dihydrolase
Protein Family
UniProt Gene Name
arcA
UniProt Synonym Gene Names
ADI; AD
UniProt Entry Name
ARCA_MYCAR

UniProt Comments for ADI

Catalytic activity: L-arginine + H2O = L-citrulline + NH3. HAMAP-Rule MF_00242

Pathway: Amino-acid degradation; L-arginine degradation via ADI pathway; carbamoyl phosphate from L-arginine: step 1/2. HAMAP-Rule MF_00242

Subunit structure: Homodimer.

Subcellular location: Cytoplasm

Potential HAMAP-Rule MF_00242.

Sequence similarities: Belongs to the arginine deiminase family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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