TIE-1/Fc Chimera, Soluble (TIE-1/Fc CHIMERA) Mouse Recombinant Protein

Recombinant murine soluble TIE-1 was fused with the Fc part of human IgG1. The recombinant mature sTIE-1/hFc is a disulfide-linked homodimeric protein. The sTIE-1/hFc monomers have a mass of approximately 105 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 130 kDa protein in SDS-PAGE under reducing conditions. The soluble receptor protein consists of the full extracellular domain (Val23-Glu749). TIE-1 (tyrosine kinase with Ig and EGF homology domains 1) and TIE-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis. Murine TIE-1 cDNA encodes a 1134 amino acid (aa) residue precursor protein with an 22 residue putative signal peptide, a 733 residue extracellular domain and a 354 residue cytoplasmic domain. Whereas two ligands have been described for TIE-2 [angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2)], so far no ligand was found for TIE-1.

1. Partanen J and DJ Dumont (1999) Curr Top Microbiol Immunol 237:159. 2. Takakura N et al, (1998) Immunity 9:677. 3. Procopio W et al, (1999) J Biol Chem 274:30196. 4. Sato et al. (1993) PNAS 90:9355 5.Gale et al., (1999) Gen Dev 13:1055

Small volumes of TIE-1/Fc Chimera recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS692035 is a recombinant protein targeting TIE-1/Fc Chimera. Also known as Mouse TIE-1/Fc Chimera, soluble. Reactive in Mouse. Host: CHO Cells.