Human VCAM-1 Active Protein – Cat# MBS692115

VCAM-1 Human Active Protein

Name: VCAM-1 Active Protein; Human; MyBioSource
Brand: MyBioSource
SKU: MBS692115
Price: 305 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS692115

VCAM is a 110 kDa cell surface integral membrane glycoprotein that belongs to the Ig-related superfamily of adhesion molecules. The primary function of VCAM-1 is the mediation of leukocyte-endothelial cell adhesion and signal transduction. VCAM-1 may play a… More details

$305 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

VCAM-1, Active Protein

Full Product Name

Human VCAM-1

Product Gene Name

VCAM-1 active protein

Product Synonym Names

Recombinant Human VCAM-1; VCAM1; CD106; INCAM-100

Sequence

FKIETTPESR YLAQIGDSVS LTCSTTGCES PFFSWRTQID SPLNGKVTNE GTTSTLTMNP VSFGNEHSYL CTATCESRKL EKGIQVEIYS FPKDPEIHLS GPLEAGKPIT VKCSVADVYP FDRLEIDLLK GDHLMKSQEF LEDADRKSLE TKSLEVTFTP VIEDIGKVLV CRAKLHIDEM DSVPTVRQAV KELQVYISPK NTVISVNPST KLQEGGSVTM TCSSEGLPAP EIFWSKKLDN GNLQHLSGNA TLTLIAMRME DSGIYVCEGV NLIGKNRKEV ELIVQEKPFT VEISPGPRIA AQIGDSVMLT CSVMGCESPS FSWRTQIDSP LSGKVRSEGT NSTLTLSPVS FENEHSYLCT VTCGHKKLEK GIQVELYSFP RDPEIEMSGG LVNGSSVTVS CKVPSVYPLD RLEIELLKGE TILENIEFLE DTDMKSLENK SLEMTFIPTI EDTGKALVCQ AKLHIDDMEF EPKQRQSTQT LYVNVAPRDT TVLVSPSSIL EEGSSVNMTC LSQGFPAPKI LWSRQLPNGE LQPLSENATL TLISTKMEDS GVYLCEGINQ AGRSRKEVEL IIQVTPKDIK LTAFPSESVK EGDTVIISCT CGNVPETWII LKKKAETGDT VLKSIDGAYT IRKAQL KDAG VYECESKNKV GSQLRSLTLD VQGRENNKDY FSP

Host

HEK 293 cells

Form/Format

Lyophilized(10mM Sodium Phosphate, pH 7.0)

Purity/Purification

> 98% by SDS-PAGE & HPLC analyses

Preparation and Storage

The lyophilized protein is stable at room temperature for 1 month and at 4°C for 3 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C.
AVOID REPEATED FREEZE AND THAW CYCLES!

Molecular Weight

74.1 kDa

Sequence Positions

674

Chromosome Location

Chromosome: 1; NC_000001.11 (100719640..100739045). Location: 1p32-p31

OMIM

192225

3D Structure

ModBase 3D Structure for P19320

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Endotoxin level

< 0.1 ng /ug of protein (<1EU/ug).

Reconstitution

Centrifuge the vial prior to opening. Reconstitutein water to a concentration of 0.1-1.0 mg/m. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Biological Activity

Determined by its ability to support the adhesion of human U937 cells. The expected ED50 for this effect is 0.8-1.0 ug/ml

Product Categories/Family for VCAM-1 active protein

Cytokines & Growth Factors

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Other Notes

Small volumes of VCAM-1 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for VCAM-1 purchase

MBS692115 is an active protein targeting VCAM-1. Also known as Human VCAM-1. Host: HEK 293 cells.

Additional Details

NCBI/Uniprot data below describe general gene information for VCAM-1. It may not necessarily be applicable to this product.

NCBI GI #

4507875

NCBI GeneID

7412

NCBI Accession #

NP_542413.1

UniProt Primary Accession #

P19320

UniProt Secondary Accession #

Q6NUP8; A8K6R7; B4DKS4; E9PDD1

UniProt Related Accession #

P19320

GenBank Nucleotide #

NM_001078.3

NCBI Official Full Name

vascular cell adhesion protein 1 isoform a

NCBI Official Synonym Full Names

vascular cell adhesion molecule 1

NCBI Official Symbol

VCAM1

NCBI Official Synonym Symbols

CD106; INCAM-100

UniProt Protein Name

Vascular cell adhesion protein 1

UniProt Synonym Protein Names

INCAM-100

Protein Family

Vascular cell adhesion protein

UniProt Gene Name

VCAM1

UniProt Synonym Gene Names

L1CAM; V-CAM 1; VCAM-1

UniProt Entry Name

VCAM1_HUMAN

NCBI Protein Information

vascular cell adhesion protein 1; CD106 antigen

NCBI Summary for VCAM-1

This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2010]

UniProt Comments for VCAM-1

Function: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.

Subunit structure: Binds to ECMV-D capsid proteins and acts as a receptor for this virus

By similarity.

Subcellular location: Membrane; Single-pass type I membrane protein.

Tissue specificity: Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.

Induction: By cytokines (e.g. IL-1, TNF-alpha).

Domain: Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion. Ref.12

Post-translational modification: Sialoglycoprotein.

Sequence similarities: Contains 7 Ig-like C2-type (immunoglobulin-like) domains.

Research Articles on VCAM-1

1. The C allele of the rs1041163 VCAM1 gene may be associated with long-term renal allograft failure and graft loss, as well as increased morbidity after transplantation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with VCAM-1 active protein

Reference Product	PubMed Publications
CD44 active protein	>13 publications with VCAM-1 and CD44
ITGA4 active protein	>7 publications with VCAM-1 and ITGA4
ITGB2 active protein	>5 publications with VCAM-1 and ITGB2
VEGFA active protein	>3 publications with VCAM-1 and VEGFA
ITGB1 active protein	>2 publications with VCAM-1 and ITGB1
ITGAD active protein	>1 publications with VCAM-1 and ITGAD
SRC active protein	>1 publications with VCAM-1 and SRC

Pathways associated with VCAM-1 active protein

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
African Trypanosomiasis Pathway antibodies	African Trypanosomiasis Pathway Diagram
African Trypanosomiasis Pathway antibodies	African Trypanosomiasis Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cellular Roles Of Anthrax Toxin Pathway antibodies	Cellular Roles Of Anthrax Toxin Pathway Diagram
Cytokine Signaling In Immune System Pathway antibodies	Cytokine Signaling In Immune System Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram
HTLV-I Infection Pathway antibodies	HTLV-I Infection Pathway Diagram
HTLV-I Infection Pathway antibodies	HTLV-I Infection Pathway Diagram

Diseases associated with VCAM-1 active protein

Disease Name	Pubmed Publications
Inflammation Antibodies	>64 publications with VCAM-1 and Inflammation
Cardiovascular Diseases Antibodies	>46 publications with VCAM-1 and Cardiovascular Diseases
Necrosis Antibodies	>44 publications with VCAM-1 and Necrosis
Neoplasms Antibodies	>35 publications with VCAM-1 and Neoplasms
Atherosclerosis Antibodies	>31 publications with VCAM-1 and Atherosclerosis
Hypertension Antibodies	>11 publications with VCAM-1 and Hypertension
Heart Diseases Antibodies	>10 publications with VCAM-1 and Heart Diseases
Kidney Diseases Antibodies	>10 publications with VCAM-1 and Kidney Diseases
Myocardial Ischemia Antibodies	>8 publications with VCAM-1 and Myocardial Ischemia
Fibrosis Antibodies	>7 publications with VCAM-1 and Fibrosis

Organs/Tissues associated with VCAM-1 active protein

Organ/Tissue Name	Pubmed Publications
Vascular Antibodies	>169 publications with VCAM-1 and Vascular
Blood Antibodies	>97 publications with VCAM-1 and Blood
Kidney Antibodies	>20 publications with VCAM-1 and Kidney
Embryonic Tissue Antibodies	>19 publications with VCAM-1 and Embryonic Tissue
Brain Antibodies	>16 publications with VCAM-1 and Brain
Liver Antibodies	>13 publications with VCAM-1 and Liver
Heart Antibodies	>12 publications with VCAM-1 and Heart
Lung Antibodies	>9 publications with VCAM-1 and Lung
Placenta Antibodies	>7 publications with VCAM-1 and Placenta
Skin Antibodies	>7 publications with VCAM-1 and Skin