Rabbit Anti-TFF-2 Polyclonal Antibody – Cat# MBS695054

TFF-2 Antibody – Rabbit Polyclonal

Name: TFF-2 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS695054
Price: 245 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS695054

Produced from sera of rabbits immunized with highly pure recombinant Human TFF-2. Anti-Human TFF-2 specific antibody was purified by affinity chromatography employing an immobilized Human TFF-2 matrix. More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

TFF-2, Polyclonal Antibody

Full Product Name

Anti-human TFF-2

Product Gene Name

anti-TFF-2 antibody

Product Synonym Names

Anti-Human TFF-2

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human

Form/Format

Purified

Preparation and Storage

At -20 degree C

Molecular Weight

14,284 Da

OMIM

182590

3D Structure

ModBase 3D Structure for Q03403

Immunogen

Recombinant Human TFF-2

Applications Tested/Suitable for anti-TFF-2 antibody

Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

Application Notes for anti-TFF-2 antibody

Immunohistochemistery: This antibody stained formalin-fixed, paraffin-embedded sections of human normal gastric mucosa. The recommended concentration is 0.25mg/ml with an overnight incubation at 4 degree C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.

Sandwich ELISA: To detect Human TFF-2 by sandwich ELISA (using 100 mul/well antibody solution) a concentration of 0.5 - 2.0 mug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human TFF-2 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human TFF-2.

Western Blot: To detect Human TFF-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 mug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human TFF-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Other Notes

Small volumes of anti-TFF-2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TFF-2 purchase

MBS695054 is an antibody targeting TFF-2. Also known as Anti-human TFF-2. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TFF-2. It may not necessarily be applicable to this product.

NCBI GI #

2833194

NCBI GeneID

7032

NCBI Accession #

Q03403.2

UniProt Primary Accession #

Q03403

UniProt Secondary Accession #

Q15854

UniProt Related Accession #

Q03403

NCBI Official Full Name

Trefoil factor 2

NCBI Official Synonym Full Names

trefoil factor 2

NCBI Official Symbol

TFF2

NCBI Official Synonym Symbols

SP; SML1

UniProt Protein Name

Trefoil factor 2

UniProt Synonym Protein Names

Spasmolysin; Spasmolytic polypeptide; SP

UniProt Gene Name

TFF2

UniProt Synonym Gene Names

SML1; SP

UniProt Entry Name

TFF2_HUMAN

NCBI Protein Information

trefoil factor 2; spasmolysin; spasmolytic protein 1; spasmolytic polypeptide

NCBI Summary for TFF-2

Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. The encoded protein inhibits gastric acid secretion. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008]

UniProt Comments for TFF-2

TFF2: Inhibits gastrointestinal motility and gastric acid secretion. Could function as a structural component of gastric mucus, possibly by stabilizing glycoproteins in the mucus gel through interactions with carbohydrate side chains.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: extracellular space

Molecular Function: protein binding; CXCR4 chemokine receptor binding

Biological Process: negative regulation of inflammatory response; digestion; positive regulation of cell proliferation; calcium-mediated signaling; negative regulation of macrophage activation; regulation of cell migration

Research Articles on TFF-2

1. Significantly higher levels of TFF2 were in patients with Multiple Organ Dysfunction Syndrome.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TFF-2 antibody

Reference Product	PubMed Publications
MUC6 antibody	>26 publications with TFF-2 and MUC6
MUC2 antibody	>21 publications with TFF-2 and MUC2
MUC5B antibody	>11 publications with TFF-2 and MUC5B
EGF antibody	>6 publications with TFF-2 and EGF
GKN2 antibody	>6 publications with TFF-2 and GKN2
FGF7 antibody	>2 publications with TFF-2 and FGF7

Diseases associated with anti-TFF-2 antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>44 publications with TFF-2 and Inflammation
Atrophy Antibodies	>21 publications with TFF-2 and Atrophy
Lung Diseases Antibodies	>17 publications with TFF-2 and Lung Diseases
Hyperplasia Antibodies	>17 publications with TFF-2 and Hyperplasia
Precancerous Conditions Antibodies	>12 publications with TFF-2 and Precancerous Conditions
Cell Transformation, Neoplastic Antibodies	>12 publications with TFF-2 and Cell Transformation, Neoplastic
Neoplasm Metastasis Antibodies	>8 publications with TFF-2 and Neoplasm Metastasis
Necrosis Antibodies	>7 publications with TFF-2 and Necrosis
Liver Neoplasms Antibodies	>7 publications with TFF-2 and Liver Neoplasms
Liver Diseases Antibodies	>5 publications with TFF-2 and Liver Diseases

Organs/Tissues associated with anti-TFF-2 antibody

Organ/Tissue Name	Pubmed Publications
Stomach Antibodies	>136 publications with TFF-2 and Stomach
Intestine Antibodies	>54 publications with TFF-2 and Intestine
Larynx Antibodies	>12 publications with TFF-2 and Larynx
Kidney Antibodies	>2 publications with TFF-2 and Kidney
Connective Tissue Antibodies	>2 publications with TFF-2 and Connective Tissue