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IL-6 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS695087

Produced from sera of goats pre-immunized with highly pure recombinant Rat IL-6. Anti-Rat IL-6 specific antibody was purified by affinity chromatography employing immobilized Rat IL-6 matrix. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
IL-6, Polyclonal Antibody
Full Product Name
Anti-rat IL-6
Product Gene Name
Product Synonym Names
Anti-Rat IL-6
Clonality
Polyclonal
Isotype
Goat IgG
Host
Goat
Species Reactivity
Rat
Form/Format
A sterile antibody solution was lyophilized from PBS, pH 7.2.
Preparation and Storage
At -20 degree C
Molecular Weight
Approximately 150 kDa
3D Structure
ModBase 3D Structure for P20607
Immunogen
E.coli derived recombinant Rat IL-6.
Reconstitution
Centrifuge vial prior to opening. Reconstitute in sterile water to concentration of 0.1-1.0 mg/ml.
Neutralization
To yield one-half maximal inhibitions [ND50] of the biological activity of Rat IL-60(0.10ng/ml), a concentration of 0.002-0.005 ug/ml antibody is required.
Applications Tested/Suitable for anti-IL-6 antibody
Immunohistochemistery (IHC), Neutralization, ELISA (EIA), Western Blot (WB)
Application Notes for anti-IL-6 antibody
Sandwich ELISA: To detect Rat IL-6 by sandwich ELISA (using 100 mul/well) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat IL-6 as a detection antibody, allows the detection of at least 2000-4000 pg/ml of recombinant Rat IL-6.

Western Blot: To detect Rat IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat IL-6 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Optimal dilutions should be determined by each laboratory for each application.

Other Notes

Small volumes of anti-IL-6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL-6 purchase

MBS695087 is an antibody targeting IL-6. Also known as Anti-rat IL-6. Reactive in Rat. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for IL-6. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
Interleukin-6
NCBI Official Synonym Full Names
interleukin 6
NCBI Official Symbol
Il6
NCBI Official Synonym Symbols
ILg6; Ifnb2
UniProt Protein Name
Interleukin-6
UniProt Gene Name
Il6
UniProt Synonym Gene Names
Il-6; IL-6
UniProt Entry Name
IL6_RAT
NCBI Protein Information
interleukin-6; IL-6; Interleukin 6 (interferon, beta 2)

NCBI Summary for IL-6

a cytokine involved in development and possibly in neurodegenerative processes [RGD, Feb 2006]

UniProt Comments for IL-6

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation ().
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-IL-6 antibody

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