Goat Anti-NP-1 Polyclonal Antibody – Cat# MBS695105

NP-1 Antibody – Goat Polyclonal

Name: NP-1 Antibody; Polyclonal; Goat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS695105
Price: 245 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS695105

Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hNP-1. Anti-Human NP-1 specific antibody was purified by affinity chromatography and then biotinylated. More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

NP-1, Polyclonal Antibody

Full Product Name

Biotinylated Anti-hNP-1

Product Gene Name

anti-NP-1 antibody

Product Synonym Names

Biotinylated Anti-Human NP-1

Clonality

Polyclonal

Host

Goat

Species Reactivity

Human

Form/Format

Biotin

Preparation and Storage

At -20 degree C

Molecular Weight

10,201 Da

OMIM

125220

3D Structure

ModBase 3D Structure for P59665

Immunogen

Recombinant Human NP-1

Applications Tested/Suitable for anti-NP-1 antibody

ELISA (EIA), Western Blot (WB)

Application Notes for anti-NP-1 antibody

Direct Detection: To detect hNP-1 by direct ELISA (using 100 mul/well antibody solution) a concentration of 0.25 - 1.0 mug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNP-1.

Sandwich ELISA: To detect hNP-1 by sandwich ELISA (using 100 mul/well antibody solution) a concentration of 0.25 - 1.0 mug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human NP-1 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNP-1.

Western Blot: To detect hNP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNP-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Other Notes

Small volumes of anti-NP-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NP-1 purchase

MBS695105 is an antibody targeting NP-1. Also known as Biotinylated Anti-hNP-1. Reactive in Human. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for NP-1. It may not necessarily be applicable to this product.

NCBI GI #

30316322

NCBI GeneID

728358

NCBI Accession #

P59665.1

UniProt Primary Accession #

P59665

UniProt Secondary Accession #

P11479; Q14125; Q6EZF6

UniProt Related Accession #

P59665

NCBI Official Full Name

Neutrophil defensin 1

NCBI Official Synonym Full Names

defensin, alpha 1B

NCBI Official Symbol

DEFA1B

NCBI Official Synonym Symbols

HP1; HP-1; HNP-1

UniProt Protein Name

Neutrophil defensin 1

UniProt Synonym Protein Names

Defensin, alpha 1; HNP-1; HP-1; HP1

UniProt Gene Name

DEFA1

UniProt Synonym Gene Names

DEF1; DEFA2; MRS; HP-1; HP1; HP-2; HP2

UniProt Entry Name

DEF1_HUMAN

NCBI Protein Information

neutrophil defensin 1

NCBI Summary for NP-1

Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2014]

UniProt Comments for NP-1

defensin, alpha 1: Defensin 1 and defensin 2 have antibacterial, fungicide and antiviral activities. Has antimicrobial activity against Gram- negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. Belongs to the alpha-defensin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: extracellular matrix; extracellular space; Golgi lumen; extracellular region

Biological Process: defense response to Gram-positive bacterium; estrogen receptor signaling pathway; innate immune response in mucosa; killing of cells of another organism; antibacterial humoral response; innate immune response; immune response; chemotaxis; defense response to fungus; defense response to virus

Research Articles on NP-1

1. DEFA1B encodes an antibacterial peptide that is bacteriocidal against S. aureus, E. coli, and P. aeruginosa.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-NP-1 antibody

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