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MCP-2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS695176

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mMCP-2. Anti-Murine MCP-2 specific antibody was purified by affinity chromatography and then biotinylated. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
MCP-2, Polyclonal Antibody
Full Product Name
Biotinylated Anti-mMCP-2
Product Gene Name
Product Synonym Names
Biotinylated Anti-Murine MCP-2
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Mouse
Form/Format
Biotin
Preparation and Storage
At -20 degree C
Molecular Weight
11,017 Da
3D Structure
ModBase 3D Structure for Q9Z121
Immunogen
Recombinant Murine MCP-2
Applications Tested/Suitable for anti-MCP-2 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-MCP-2 antibody
Direct Detection: To detect mMCP-2 by direct ELISA (using 100 mul/well antibody solution) a concentration of 0.25 - 1.0 mug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMCP-2.

Sandwich ELISA: To detect mMCP-2 by sandwich ELISA (using 100 mul/well antibody solution) a concentration of 0.25 - 1.0 mug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine MCP-2 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMCP-2.

Western Blot: To detect mMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Other Notes

Small volumes of anti-MCP-2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MCP-2 purchase

MBS695176 is an antibody targeting MCP-2. Also known as Biotinylated Anti-mMCP-2. Reactive in Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MCP-2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
NCBI Official Full Name
C-C motif chemokine 8
NCBI Official Symbol
LOC102641794
UniProt Protein Name
C-C motif chemokine 8
UniProt Synonym Protein Names
Monocyte chemoattractant protein 2; Monocyte chemotactic protein 2; MCP-2; Small-inducible cytokine A8
Protein Family
UniProt Gene Name
Ccl8
UniProt Synonym Gene Names
Mcp2; Scya8; MCP-2
UniProt Entry Name
CCL8_MOUSE
NCBI Protein Information
c-C motif chemokine 8-like

UniProt Comments for MCP-2

CCL8: Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted; Chemokine

Cellular Component: extracellular space; extracellular region

Molecular Function: heparin binding; chemokine activity; cytokine activity

Biological Process: immune response; chemotaxis; inflammatory response; positive regulation of myoblast differentiation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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