Rabbit Anti-MIP-4 Polyclonal Antibody – Cat# MBS695267

MIP-4 Antibody – Rabbit Polyclonal

Name: MIP-4 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS695267
Price: 245 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS695267

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMIP-4. Anti-Human MIP-4 specific antibody was purified by affinity chromatography employing immobilized hMIP-4 matrix. More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

MIP-4, Polyclonal Antibody

Full Product Name

Anti-human MIP-4

Product Gene Name

anti-MIP-4 antibody

Product Synonym Names

Anti-Human MIP-4

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human

Form/Format

Purified

Preparation and Storage

At -20 degree C

Molecular Weight

9,849 Da

OMIM

603757

3D Structure

ModBase 3D Structure for P55774

Immunogen

Recombinant Human MIP-4

Applications Tested/Suitable for anti-MIP-4 antibody

Immunohistochemistry (IHC) Formalin/Paraffin, Neutralization, ELISA (EIA), Western Blot (WB)

Application Notes for anti-MIP-4 antibody

Immunohistochemistery: This antibody stained formalin-fixed, paraffin-embedded sections of human normal placenta. The recommended concentration is 5.0 ug/mL with an overnight incubation at 4 degree C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 buffer is recommended. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Information and photo are courtesy of Flagship Biosciences, LLC - Histology Services.

Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-4 (100.0 ng/ml), a concentration of 8.0-10.0 ug/ml of this antibody is required.

Sandwich ELISA: To detect hMIP-4 by sandwich ELISA (using 100 mul/well antibody solution) a concentration of 0.5 - 2.0 mug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MIP-4 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-4.

Western Blot: To detect hMIP-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Other Notes

Small volumes of anti-MIP-4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MIP-4 purchase

MBS695267 is an antibody targeting MIP-4. Also known as Anti-human MIP-4. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MIP-4. It may not necessarily be applicable to this product.

NCBI GI #

2493666

NCBI GeneID

6362

NCBI Accession #

P55774.1

UniProt Primary Accession #

P55774

UniProt Secondary Accession #

Q53X71; B5BUM2

UniProt Related Accession #

P55774

NCBI Official Full Name

C-C motif chemokine 18

NCBI Official Synonym Full Names

chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated)

NCBI Official Symbol

CCL18

NCBI Official Synonym Symbols

CKb7; PARC; AMAC1; DCCK1; MIP-4; AMAC-1; DC-CK1; SCYA18

UniProt Protein Name

C-C motif chemokine 18

UniProt Synonym Protein Names

Alternative macrophage activation-associated CC chemokine 1; AMAC-1; CC chemokine PARC; Dendritic cell chemokine 1; DC-CK1; Macrophage inflammatory protein 4; MIP-4; Pulmonary and activation-regulated chemokine; Small-inducible cytokine A18Cleaved into the following 3 chains:CCL18(1-68); CCL18(3-69); CCL18(4-69)

Protein Family

C-C motif chemokine

UniProt Gene Name

CCL18

UniProt Synonym Gene Names

AMAC1; DCCK1; MIP4; PARC; SCYA18; AMAC-1; DC-CK1; MIP-4

UniProt Entry Name

CCL18_HUMAN

NCBI Protein Information

C-C motif chemokine 18; CC chemokine PARC; CC chemokine ligand 18; chemokine (C-C), dendritic; dendritic cell chemokine 1; small inducible cytokine A18; small-inducible cytokine A18; macrophage inflammatory protein 4; pulmonary and activation-regulated chemokine; alternative macrophage activation-associated CC chemokine 1; small inducible cytokine subfamily A (Cys-Cys), member 18, pulmonary and activation-regulated

NCBI Summary for MIP-4

This antimicrobial gene is one of several Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for naive T cells, CD4+ and CD8+ T cells and nonactivated lymphocytes, but not for monocytes or granulocytes. This chemokine attracts naive T lymphocytes toward dendritic cells and activated macrophages in lymph nodes. It may play a role in both humoral and cell-mediated immunity responses. [provided by RefSeq, Sep 2014]

UniProt Comments for MIP-4

CCL18: Chemotactic factor that attracts lymphocytes but not monocytes or granulocytes. May be involved in B-cell migration into B-cell follicles in lymph nodes. Attracts naive T-lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T-cells, CD4+ and CD8+ T-cells and thus may play a role in both humoral and cell-mediated immunity responses. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis; Chemokine

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular space

Molecular Function: protein binding; chemokine activity

Biological Process: cell-cell signaling; response to biotic stimulus; cell communication; immune response; inflammatory response; chemotaxis; signal transduction

Research Articles on MIP-4

1. CCL18 expression was positively correlated with malignancy in EC.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MIP-4 antibody

Reference Product	PubMed Publications
CCR3 antibody	>12 publications with MIP-4 and CCR3
CCR7 antibody	>10 publications with MIP-4 and CCR7
CCR5 antibody	>9 publications with MIP-4 and CCR5
CCR2 antibody	>8 publications with MIP-4 and CCR2
CCR6 antibody	>6 publications with MIP-4 and CCR6
CCR1 antibody	>4 publications with MIP-4 and CCR1
CXCR2 antibody	>2 publications with MIP-4 and CXCR2
CXCR1 antibody	>1 publications with MIP-4 and CXCR1
CXCR3 antibody	>1 publications with MIP-4 and CXCR3

Pathways associated with anti-MIP-4 antibody

Products by Pathway	Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram

Diseases associated with anti-MIP-4 antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>80 publications with MIP-4 and Inflammation
Immune System Diseases Antibodies	>69 publications with MIP-4 and Immune System Diseases
Respiratory Tract Diseases Antibodies	>50 publications with MIP-4 and Respiratory Tract Diseases
Lung Diseases Antibodies	>47 publications with MIP-4 and Lung Diseases
Fibrosis Antibodies	>37 publications with MIP-4 and Fibrosis
Necrosis Antibodies	>32 publications with MIP-4 and Necrosis
Dermatitis Antibodies	>22 publications with MIP-4 and Dermatitis
Cardiovascular Diseases Antibodies	>19 publications with MIP-4 and Cardiovascular Diseases
Carcinoma Antibodies	>15 publications with MIP-4 and Carcinoma
Respiratory Hypersensitivity Antibodies	>13 publications with MIP-4 and Respiratory Hypersensitivity

Organs/Tissues associated with anti-MIP-4 antibody

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>55 publications with MIP-4 and Lung
Skin Antibodies	>29 publications with MIP-4 and Skin
Vascular Antibodies	>28 publications with MIP-4 and Vascular
Bone Antibodies	>18 publications with MIP-4 and Bone
Intestine Antibodies	>4 publications with MIP-4 and Intestine
Connective Tissue Antibodies	>3 publications with MIP-4 and Connective Tissue
Uterus Antibodies	>1 publications with MIP-4 and Uterus
Placenta Antibodies	>1 publications with MIP-4 and Placenta
Uncharacterized Tissue Antibodies	>1 publications with MIP-4 and Uncharacterized Tissue