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WNT-3a Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS695374

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hWNT-3a. Anti-Human WNT-3a specific antibody was purified by affinity chromatography and then biotinylated. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
WNT-3A, Polyclonal Antibody
Full Product Name
Biotinylated Anti-hWNT-3A
Product Gene Name
anti-WNT-3a antibody
Product Synonym Names
Biotinylated Anti-Human WNT-3a
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Biotin
Preparation and Storage
At -20 degree C
Molecular Weight
42,402 Da
Applications Tested/Suitable for anti-WNT-3a antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-WNT-3a antibody
Direct Detection: To detect hWNT-3a by direct ELISA (using 100 mul/well antibody solution) a concentration of 0.25 - 1.0 mug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-3a.

Sandwich ELISA: To detect hWNT-3a by sandwich ELISA (using 100 mul/well antibody solution) a concentration of 0.25 - 1.0 mug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human WNT-3a as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-3a.

Western Blot: To detect hWNT-3a by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 mug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-3a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Other Notes

Small volumes of anti-WNT-3a antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for WNT-3a purchase

MBS695374 is an antibody targeting WNT-3a. Also known as Biotinylated Anti-hWNT-3A. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for WNT-3a. It may not necessarily be applicable to this product.

NCBI GI #
5821261
NCBI GeneID
395396
NCBI Accession #
BAA83743.1
UniProt Secondary Accession #
Q9PWH1
UniProt Related Accession #
Q2LMP1
NCBI Official Full Name
Wnt-3a
NCBI Official Synonym Full Names
wingless-type MMTV integration site family, member 3A
NCBI Official Symbol
WNT3A
NCBI Official Synonym Symbols
WNT3; WNT-3A
UniProt Protein Name
Protein Wnt-3a
Protein Family
Protein
UniProt Gene Name
WNT3A
UniProt Entry Name
WNT3A_CHICK
NCBI Protein Information
protein Wnt-3a; Protein Wnt; Wnt-3a homolog; Wnt3a variant 3; wingless-type MMTV integration site family member 3a; wingless-type MMTV integration site family, member 3

UniProt Comments for WNT-3a

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters (). Isoform 1 regulates chick apical ectodermal ridge formation.
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Research Articles on WNT-3a

1. SMAD1 has a role in migration of cardiac progenitor cells by integrating BMP2 and Wnt3a signals

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-WNT-3a antibody

 Reference Product  PubMed Publications
 AXIN2 antibody  >48 publications with WNT-3a and AXIN2
 FZD1 antibody  >20 publications with WNT-3a and FZD1
 SFRP2 antibody  >17 publications with WNT-3a and SFRP2
 FZD4 antibody  >17 publications with WNT-3a and FZD4
 FZD7 antibody  >15 publications with WNT-3a and FZD7
 FZD2 antibody  >13 publications with WNT-3a and FZD2
 FZD10 antibody  >10 publications with WNT-3a and FZD10
 WIF1 antibody  >7 publications with WNT-3a and WIF1
 NOG antibody  >3 publications with WNT-3a and NOG

Pathways associated with anti-WNT-3a antibody

 Products by Pathway  Pathway Diagram
 Hedgehog Signaling Pathway antibodies  Hedgehog Signaling Pathway Diagram
 Hedgehog Signaling Pathway antibodies  Hedgehog Signaling Pathway Diagram
 Melanogenesis Pathway antibodies  Melanogenesis Pathway Diagram
 Melanogenesis Pathway antibodies  Melanogenesis Pathway Diagram
 Wnt Signaling Pathway antibodies  Wnt Signaling Pathway Diagram
 Wnt Signaling Pathway And Pluripotency antibodies  Wnt Signaling Pathway And Pluripotency Diagram
 Wnt Signaling Pathway antibodies  Wnt Signaling Pathway Diagram
 Wnt Signaling Pathway antibodies  Wnt Signaling Pathway Diagram

Diseases associated with anti-WNT-3a antibody

 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >33 publications with WNT-3a and Breast Neoplasms
 Necrosis Antibodies  >28 publications with WNT-3a and Necrosis
 Inflammation Antibodies  >26 publications with WNT-3a and Inflammation
 Fibrosis Antibodies  >26 publications with WNT-3a and Fibrosis
 Congenital Abnormalities Antibodies  >25 publications with WNT-3a and Congenital Abnormalities
 Liver Diseases Antibodies  >22 publications with WNT-3a and Liver Diseases
 Liver Neoplasms Antibodies  >16 publications with WNT-3a and Liver Neoplasms
 Lung Diseases Antibodies  >15 publications with WNT-3a and Lung Diseases
 Brain Diseases Antibodies  >15 publications with WNT-3a and Brain Diseases
 Neoplasms, Experimental Antibodies  >13 publications with WNT-3a and Neoplasms, Experimental

Organs/Tissues associated with anti-WNT-3a antibody

 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >145 publications with WNT-3a and Embryonic Tissue
 Head Antibodies  >21 publications with WNT-3a and Head
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