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IL-5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS695381

Produced from sera of rabbits pre-immunized with highly pure recombinant Human IL-5. Anti-Human IL-5 specific antibody was purified by affinity chromatography and then biotinylated. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
IL-5, Polyclonal Antibody
Full Product Name
Biotinylated Anti-hIL-5
Product Gene Name
anti-IL-5 antibody
Product Synonym Names
Biotinylated Anti-Human IL-5
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Biotin
Preparation and Storage
At -20 degree C
Molecular Weight
15,238 Da
OMIM
147850
3D Structure
ModBase 3D Structure for P05113
Immunogen
Recombinant Human IL-5
Applications Tested/Suitable for anti-IL-5 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-IL-5 antibody
Direct Detection: To detect Human IL-5 by direct ELISA (using 100 mul/well antibody solution) a concentration of 0.25 - 1.0 mug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-5.

Sandwich ELISA: To detect Human IL-5 by sandwich ELISA (using 100 mul/well antibody solution) a concentration of 0.25 - 1.0 mug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IL-5 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-5.

Western Blot: To detect Human IL-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Other Notes

Small volumes of anti-IL-5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL-5 purchase

MBS695381 is an antibody targeting IL-5. Also known as Biotinylated Anti-hIL-5. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for IL-5. It may not necessarily be applicable to this product.

NCBI GI #
124341
NCBI GeneID
3567
NCBI Accession #
P05113.1
UniProt Primary Accession #
P05113
UniProt Secondary Accession #
Q13840
UniProt Related Accession #
P05113
NCBI Official Full Name
Interleukin-5
NCBI Official Synonym Full Names
interleukin 5
NCBI Official Symbol
IL5
NCBI Official Synonym Symbols
EDF; TRF; IL-5
UniProt Protein Name
Interleukin-5
UniProt Synonym Protein Names
B-cell differentiation factor I; Eosinophil differentiation factor; T-cell replacing factor; TRF
UniProt Gene Name
IL5
UniProt Synonym Gene Names
IL-5; TRF
UniProt Entry Name
IL5_HUMAN
NCBI Protein Information
interleukin-5; T-cell replacing factor; B-cell differentiation factor I; eosinophil differentiation factor; colony-stimulating factor, eosinophil

NCBI Summary for IL-5

This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. [provided by RefSeq, Jul 2013]
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UniProt Comments for IL-5

IL5: Factor that induces terminal differentiation of late- developing B-cells to immunoglobulin secreting cells. Belongs to the IL-5 family.

Protein type: Secreted, signal peptide; Cytokine; Secreted

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space; extracellular region

Molecular Function: interleukin-5 receptor binding; protein binding; growth factor activity; cytokine activity

Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; positive regulation of B cell proliferation; positive regulation of JAK-STAT cascade; positive regulation of transcription factor activity; immune response; positive regulation of immunoglobulin secretion; inflammatory response; positive regulation of eosinophil differentiation
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Research Articles on IL-5

1. 11beta-HSD2 expression was decreased in allergic nasal mucosa. In cultured epithelial cells treated with IL-4, IL-5, IL-13, and IL-17A, 11beta-HSD1 expression and activity increased in parallel with the expression levels of CYP11B1 and cortisol

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-IL-5 antibody

 Reference Product  PubMed Publications
 IL4 antibody  >213 publications with IL-5 and IL4
 IL2 antibody  >101 publications with IL-5 and IL2
 IL3 antibody  >50 publications with IL-5 and IL3
 JUN antibody  >36 publications with IL-5 and JUN
 IL9 antibody  >25 publications with IL-5 and IL9
 IL5RA antibody  >24 publications with IL-5 and IL5RA
 CSF2RB antibody  >5 publications with IL-5 and CSF2RB
 SDCBP antibody  >1 publications with IL-5 and SDCBP
 FOS antibody  >1 publications with IL-5 and FOS

Pathways associated with anti-IL-5 antibody

 Products by Pathway  Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies  Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram

Diseases associated with anti-IL-5 antibody

 Disease Name  Pubmed Publications
 Hypersensitivity Antibodies  >125 publications with IL-5 and Hypersensitivity
 Asthma Antibodies  >109 publications with IL-5 and Asthma
 Inflammation Antibodies  >107 publications with IL-5 and Inflammation
 Respiratory Hypersensitivity Antibodies  >92 publications with IL-5 and Respiratory Hypersensitivity
 Lung Diseases Antibodies  >88 publications with IL-5 and Lung Diseases
 Eosinophilia Antibodies  >68 publications with IL-5 and Eosinophilia
 Necrosis Antibodies  >58 publications with IL-5 and Necrosis
 Fibrosis Antibodies  >13 publications with IL-5 and Fibrosis
 Bronchial Hyperreactivity Antibodies  >13 publications with IL-5 and Bronchial Hyperreactivity
 Hyperplasia Antibodies  >10 publications with IL-5 and Hyperplasia

Organs/Tissues associated with anti-IL-5 antibody

 Organ/Tissue Name  Pubmed Publications
 Thymus Antibodies  >8 publications with IL-5 and Thymus
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