DKK3 Recombinant Protein – Cat# MBS2030843

Dickkopf Related Protein 3 (DKK3) Recombinant Protein

Name: DKK3 Recombinant Protein; MyBioSource
Brand: MyBioSource
SKU: MBS2030843
Price: 195 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2030843

MBS2030843 is a recombinant protein targeting DKK3. Also known as Recombinant Dickkopf Related Protein 3 (DKK3). Host: E ColiSource: Prokaryotic expression. More details

$195 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Dickkopf Related Protein 3 (DKK3), Recombinant Protein

Full Product Name

Recombinant Dickkopf Related Protein 3 (DKK3)

Product Gene Name

DKK3 recombinant protein

Sequence

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHH H SSGLVPRGSH MASMTGGQQM GRGSEF- APAPTATSA PVKPGPALSY PQEEATLNEM FREVEELMED TQHKLRSAVE EMEAEEAAAK ASSEVNLANL PPSYHNETNT DTKVGNNTIH VHREIHKITN NQTGQMVFSE TVITSVGDEE GRRSHECIID EDCGPSMYCQ FASFQYTCQP CRGQRMLCTR DSECCGDQLC VWGHCTKMAT RGSNGTICDN QRDCQPGLCC AFQRGLLFPV CTPLPVEGEL CHDPASRLLD LITWELEPDG ALDRCPCASG LLCQPHSHSL VYVCKPTFVG SRDQDGEILL PREVPDEYEV GSFMEEVRQE LEDLERSLTE EMALREPAAA AAALLGGEEI

Host

Host: E Coli
Source: Prokaryotic expression

Form/Format

Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Purity/Purification

>95%

Concentration

4.8 (lot specific)

Preparation and Storage

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

Matching Pairs

Unconjugated Antibody: DKK3 (MBS2026776)
Immunogen: DKK3 (MBS2030843)

Matching Pairs

APC-CY7 Conjugated Antibody: DKK3 (MBS2071299)
Immunogen: DKK3 (MBS2030843)

Molecular Weight

38,390 Da

OMIM

605416

Supply Chain Verification

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Predicted Molecular Mass

40.1kDa

Accurate Molecular Mass (KD)

46kDa

Endotoxin

<1.0EU per 1ug (determined by the LAL method)

Expression System

Prokaryotic expression

Tag

two N-terminal Tags, His-tag and T7-tag

Organism Species

Homo sapiens (Human)

Fragment

Ala22~Ile350

Usage

Reconstitute in sterile PBS, pH7.2-pH7.4.

ISO Certification

Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.

Applications Tested/Suitable for DKK3 recombinant protein

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

Other Notes

Small volumes of DKK3 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DKK3. It may not necessarily be applicable to this product.

NCBI GI #

66346689

NCBI GeneID

27122

NCBI Accession #

NP_001018067.1

UniProt Secondary Accession #

Q9ULB7; A8K1I2; D3DQW1

UniProt Related Accession #

Q9UBP4

GenBank Nucleotide #

NM_001018057.1

NCBI Official Full Name

dickkopf-related protein 3

NCBI Official Synonym Full Names

dickkopf WNT signaling pathway inhibitor 3

NCBI Official Symbol

DKK3

NCBI Official Synonym Symbols

RIG; REIC

UniProt Protein Name

Dickkopf-related protein 3

Protein Family

Dickkopf-related protein

UniProt Gene Name

DKK3

UniProt Synonym Gene Names

REIC; Dickkopf-3; Dkk-3; hDkk-3

UniProt Entry Name

DKK3_HUMAN

NCBI Protein Information

dickkopf-related protein 3

NCBI Summary for DKK3

This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

UniProt Comments for DKK3

DKK3: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Belongs to the dickkopf family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11p15.2

Cellular Component: extracellular space

Biological Process: anatomical structure morphogenesis; Wnt receptor signaling pathway; adrenal gland development; negative regulation of aldosterone biosynthetic process; negative regulation of transcription, DNA-dependent

Research Articles on DKK3

1. Loss of DKK3 protein expression was significantly associated with poor survival in patients with gastric cancer and was strongly correlated with the TNM stage.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with DKK3 recombinant protein

Reference Product	PubMed Publications
DKK1 recombinant protein	>53 publications with DKK3 and DKK1
DKK2 recombinant protein	>40 publications with DKK3 and DKK2
LRP6 recombinant protein	>10 publications with DKK3 and LRP6
WNT6 recombinant protein	>5 publications with DKK3 and WNT6
PAX6 recombinant protein	>5 publications with DKK3 and PAX6
KREMEN1 recombinant protein	>5 publications with DKK3 and KREMEN1
LRP5 recombinant protein	>5 publications with DKK3 and LRP5
WNT2B recombinant protein	>1 publications with DKK3 and WNT2B

Diseases associated with DKK3 recombinant protein

Disease Name	Pubmed Publications
Carcinoma Antibodies	>59 publications with DKK3 and Carcinoma
Adenocarcinoma Antibodies	>32 publications with DKK3 and Adenocarcinoma
Prostatic Neoplasms Antibodies	>19 publications with DKK3 and Prostatic Neoplasms
Lung Neoplasms Antibodies	>17 publications with DKK3 and Lung Neoplasms
Breast Neoplasms Antibodies	>13 publications with DKK3 and Breast Neoplasms
Lung Diseases Antibodies	>13 publications with DKK3 and Lung Diseases
Leukemia Antibodies	>13 publications with DKK3 and Leukemia
Neoplasm Metastasis Antibodies	>13 publications with DKK3 and Neoplasm Metastasis
Skin Diseases Antibodies	>13 publications with DKK3 and Skin Diseases
Carcinoma, Hepatocellular Antibodies	>11 publications with DKK3 and Carcinoma, Hepatocellular

Organs/Tissues associated with DKK3 recombinant protein

Organ/Tissue Name	Pubmed Publications
Eye Antibodies	>26 publications with DKK3 and Eye
Prostate Antibodies	>26 publications with DKK3 and Prostate
Bone Antibodies	>24 publications with DKK3 and Bone
Lung Antibodies	>23 publications with DKK3 and Lung
Brain Antibodies	>17 publications with DKK3 and Brain
Embryonic Tissue Antibodies	>16 publications with DKK3 and Embryonic Tissue
Heart Antibodies	>12 publications with DKK3 and Heart
Kidney Antibodies	>11 publications with DKK3 and Kidney
Liver Antibodies	>11 publications with DKK3 and Liver
Muscle Antibodies	>11 publications with DKK3 and Muscle