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BRAK Human Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS145415

Description: CXCL14 Human Recombinant produced in E Coli is a single, non-glycosylated, Polypeptide chain containing 77 amino acids and having a molecular mass of 9.4kDa.The CXCL14 is purified by proprietary chromatographic techniques.Introduction: CXCL14 is… More details

$250 / 0.005 mg +1 FREE 8GB USB
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
BRAK, Active Protein
Full Product Name
Recombinant Human BRAK (CXCL14)
Product Gene Name
Product Synonym Names
BRAK Human; BRAK (CXCL14) Human Recombinant; C-X-C motif chemokine 14; Small-inducible cytokine B14; Chemokine BRAK; Bolekine; NJAC; KS1; Kec; BMAC; MIP-2g; SCYB14; CXCL14; BRAK; MGC10687
Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Lys-Cy s-Lys-Cys
Host
E Coli
Form/Format
CXCL14 was lyophilized after extensive dialysis against 20mM Tris-HCl, pH 8.5 and 1M NaCl.
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity/Purification
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Molecular Weight
13,078 Da
OMIM
604186
3D Structure
ModBase 3D Structure for O95715
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Solubility
It is recommended to reconstitute the lyophilized CXCL14 in sterile 18M-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity
The ED50 of CXCL14 as determined by its ability to induce calcium flux of prostaglandin E2 treated THP1 human acute monocytic leukemia cells was 1.0-10.0 ng/ml.

Product Categories/Family for BRAK active protein

Other Notes

Small volumes of BRAK active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BRAK purchase

MBS145415 is an active protein targeting BRAK. Also known as Recombinant Human BRAK (CXCL14). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for BRAK. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
C-X-C motif chemokine 14
NCBI Official Synonym Full Names
chemokine (C-X-C motif) ligand 14
NCBI Official Symbol
CXCL14
NCBI Official Synonym Symbols
KEC; KS1; BMAC; BRAK; NJAC; MIP2G; MIP-2g; SCYB14
UniProt Protein Name
C-X-C motif chemokine 14
UniProt Synonym Protein Names
Chemokine BRAK; MIP-2G; Small-inducible cytokine B14
Protein Family
UniProt Gene Name
CXCL14
UniProt Synonym Gene Names
MIP2G; NJAC; SCYB14
UniProt Entry Name
CXL14_HUMAN
NCBI Protein Information
C-X-C motif chemokine 14; CXC chemokine in breast and kidney; MIP-2 gamma; bolekine; chemokine BRAK; small inducible cytokine subfamily B (Cys-X-Cys), member 14 (BRAK); small-inducible cytokine B14; tumor-suppressing chemokine

NCBI Summary for BRAK

This antimicrobial gene belongs to the cytokine gene family which encode secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC (Cys-X-Cys) subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes but not for lymphocytes, dendritic cells, neutrophils or macrophages. It has been implicated that this cytokine is involved in the homeostasis of monocyte-derived macrophages rather than in inflammation. [provided by RefSeq, Sep 2014]
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UniProt Comments for BRAK

CXCL14: Potent chemoattractant for neutrophils, and weaker for dendritic cells. Not chemotactic for T-cells, B-cells, monocytes, natural killer cells or granulocytes. Does not inhibit proliferation of myeloid progenitors in colony formation assays. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted; Chemokine

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: Golgi apparatus; extracellular space

Molecular Function: chemokine activity

Biological Process: cell-cell signaling; immune response; negative regulation of myoblast differentiation; chemotaxis; signal transduction; inner ear development
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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