TFF-3 ELISA Kit for Human

The Trefoil Factor peptides (TFF1, TFF2 and TFF3) are secreted in the gastrointestinal tract, and appear to play an important role in intestinal mucosal defense and repair. TFF-3 is expressed by goblet cells and in the uterus, and has also been shown to be expressed in certain cancers, including colorectal, hepatocellular, and in biliary tumors. TFF3 may be useful as a molecular marker for certain types of cancer, but its role, if any, in tumorigenesis is unknown. TFF3 also promotes airway epithelial cell migration and differentiation.
Human TFF-3 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure TFF-3 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for TFF-3. It contains E Coli-expressed recombinant TFF-3. The detection antibody is a biotinylated goat polyclonal antibody specific for TFF-3. The kit is analytically validated with ready to use reagents.

Small volumes of TFF-3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS697355 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the TFF-3, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TFF-3. The ELISA analytical biochemical technique of the MBS697355 kit is based on TFF-3 antibody-TFF-3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TFF-3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TFF-3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).