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LIF ELISA Kit for Murine

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS697361

LIF is a pleiotrophic factor produced by multiple cell types, including T cells, myelomonocytic lineages, fibroblasts, liver, heart and melanoma. LIF promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Other… More details

$665 / 1 Kit +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
LIF, ELISA Kit
Full Product Name
Murine LIF Pre-Coated ELISA Kit
Product Gene Name
LIF elisa kit
Species Reactivity
Murine
Assay Type
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Detection Range
7.8pg/ml-500pg/ml
Sensitivity
<10pg/ml
Specificity
Natural and recombinant Murine LIF Pre-Coated ELISA Kit
Preparation and Storage
Store the unopened product at 2-8 degree C.
Molecular Weight
22,287 Da
3D Structure
ModBase 3D Structure for P09056
Components
Anti-Murine LIF Pre-coated 96-well strip microplate
Murine LIF Standard
Murine LIF Biotinylated antibody (100x)
Avidin-Biotin-Peroxidase Complex (100x)
Sample Diluent
Antibody Diluent
Avidin-Biotin-Peroxidase Diluent
Wash Buffer Concentrate (Powder for 1000mL)
Color Developing Reagent
Stop Solution
Plate Sealers
Interference
No detectable cross-reactivity with other relevant proteins

Typical Testing Data/Standard Curve (for reference only) of LIF elisa kit

Murine LIF ELISA Kit – Cat# MBS697361

Other Notes

Small volumes of LIF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LIF purchase

MBS697361 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the LIF, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LIF. The ELISA analytical biochemical technique of the MBS697361 kit is based on LIF antibody-LIF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LIF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LIF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for LIF. It may not necessarily be applicable to this product.

NCBI GI #
88014736
NCBI GeneID
16878
NCBI Accession #
NP_001034626.1
UniProt Primary Accession #
P09056
UniProt Related Accession #
P09056
GenBank Nucleotide #
NM_001039537.2
NCBI Official Full Name
leukemia inhibitory factor isoform b
NCBI Official Synonym Full Names
leukemia inhibitory factor
NCBI Official Symbol
Lif
UniProt Protein Name
Leukemia inhibitory factor
UniProt Synonym Protein Names
Differentiation-stimulating factor; D factor
Protein Family
Leukemia inhibitory factor
UniProt Gene Name
Lif
UniProt Synonym Gene Names
LIF; D factor
UniProt Entry Name
LIF_MOUSE
NCBI Protein Information
leukemia inhibitory factor; d factor; differentiation-stimulating factor

UniProt Comments for LIF

LIF: LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. Belongs to the LIF/OSM family.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; cytoplasm; extracellular region

Molecular Function: growth factor activity; leukemia inhibitory factor receptor binding; cytokine activity; receptor binding

Biological Process: transcription from RNA polymerase II promoter; maternal process involved in pregnancy; negative regulation of hormone secretion; leukemia inhibitory factor signaling pathway; tyrosine phosphorylation of Stat3 protein; decidualization; positive regulation of adrenocorticotropic hormone secretion; positive regulation of tyrosine phosphorylation of Stat3 protein; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; astrocyte differentiation; regulation of cell differentiation; negative regulation of meiosis; positive regulation of cell proliferation; positive regulation of macrophage differentiation; muscle morphogenesis; positive regulation of astrocyte differentiation; positive regulation of peptidyl-serine phosphorylation of STAT protein; organ regeneration; stem cell maintenance; stem cell differentiation; positive regulation of peptidyl-serine phosphorylation; positive regulation of tyrosine phosphorylation of Stat1 protein; negative regulation of angiogenesis; positive regulation of peptidyl-tyrosine phosphorylation; retina development in camera-type eye; neuron development; positive regulation of transcription from RNA polymerase II promoter; immune response; blood vessel remodeling; alveolus development; embryo implantation; lung development
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Research Articles on LIF

1. Data show that the contribution of leukemia inhibitory factor (LIF) for embryo implantation was dependent on the strains of mice.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with LIF elisa kit

 Reference Product  PubMed Publications
 Stat3 elisa kit  >561 publications with LIF and Stat3
 Lifr elisa kit  >463 publications with LIF and Lifr
 Tnf elisa kit  >271 publications with LIF and Tnf
 Il6st elisa kit  >237 publications with LIF and Il6st
 Nanog elisa kit  >184 publications with LIF and Nanog
 Stat1 elisa kit  >87 publications with LIF and Stat1
 Socs3 elisa kit  >77 publications with LIF and Socs3
 Gfap elisa kit  >69 publications with LIF and Gfap
 Kitl elisa kit  >2 publications with LIF and Kitl

Pathways associated with LIF elisa kit

 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 ESC Pluripotency Pathways antibodies  ESC Pluripotency Pathways Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 MicroRNAs In Cardiomyocyte Hypertrophy Pathway antibodies  MicroRNAs In Cardiomyocyte Hypertrophy Pathway Diagram
 PluriNetWork Pathway antibodies  PluriNetWork Pathway Diagram
 Signaling Pathways Regulating Pluripotency Of Stem Cells antibodies  Signaling Pathways Regulating Pluripotency Of Stem Cells Diagram
 Signaling Pathways Regulating Pluripotency Of Stem Cells antibodies  Signaling Pathways Regulating Pluripotency Of Stem Cells Diagram

Diseases associated with LIF elisa kit

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >280 publications with LIF and Necrosis
 Inflammation Antibodies  >276 publications with LIF and Inflammation
 Cardiovascular Diseases Antibodies  >190 publications with LIF and Cardiovascular Diseases
 Female Urogenital Diseases Antibodies  >163 publications with LIF and Female Urogenital Diseases
 Infertility, Female Antibodies  >109 publications with LIF and Infertility, Female
 Neoplasms, Experimental Antibodies  >85 publications with LIF and Neoplasms, Experimental
 Heart Diseases Antibodies  >70 publications with LIF and Heart Diseases
 Lung Diseases Antibodies  >68 publications with LIF and Lung Diseases
 Liver Diseases Antibodies  >59 publications with LIF and Liver Diseases
 Liver Neoplasms Antibodies  >46 publications with LIF and Liver Neoplasms

Organs/Tissues associated with LIF elisa kit

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >513 publications with LIF and Bone
 Embryonic Tissue Antibodies  >343 publications with LIF and Embryonic Tissue
 Bone Marrow Antibodies  >247 publications with LIF and Bone Marrow
 Liver Antibodies  >195 publications with LIF and Liver
 Skin Antibodies  >184 publications with LIF and Skin
 Spleen Antibodies  >78 publications with LIF and Spleen
 Thymus Antibodies  >48 publications with LIF and Thymus
 Intestine Antibodies  >34 publications with LIF and Intestine
 Pancreas Antibodies  >28 publications with LIF and Pancreas
 Mammary Gland Antibodies  >17 publications with LIF and Mammary Gland
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